PMID- 30509265
OWN - NLM
STAT- MEDLINE
DCOM- 20190123
LR  - 20190123
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 14
IP  - 1
DP  - 2018 Dec 4
TI  - In vitro immune responses of porcine alveolar macrophages reflect host immune 
      responses against porcine reproductive and respiratory syndrome viruses.
PG  - 380
LID - 10.1186/s12917-018-1675-x [doi]
LID - 380
AB  - BACKGROUND: Currently, an in vitro immunogenicity screening system for the 
      immunological assessment of potential porcine reproductive and respiratory 
      syndrome virus (PRRSV) vaccine candidates is highly desired. Thus, in the present 
      study, two genetically divergent PRRSVs were characterized in vitro and in vivo 
      to identify an in vitro system and immunological markers that predict the host 
      immune response. Porcine alveolar macrophages (PAMs) and peripheral blood 
      mononuclear cells (PBMCs) collected from PRRSV-negative pigs were used for in 
      vitro immunological evaluation, and the response of these cells to VR2332c or 
      JA142c were compared with those elicited in pigs challenged with the same 
      viruses. RESULTS: Compared with VR2332c or mock infection, JA142c induced 
      increased levels of type I interferons and pro-inflammatory cytokines (TNF-alpha, 
      IL-1alpha/beta, IL-6, IL-8, and IL-12) in PAMs, and these elevated levels were 
      comparable to the cytokine induction observed in PRRSV-challenged pigs. 
      Furthermore, significantly greater numbers of activated CD4(+) T cells, type I 
      helper T cells, cytotoxic T cells and total IFN-gamma(+) cells were observed in 
      JA142c-challenged pigs than in VR2332c- or mock-challenged pigs. CONCLUSIONS: 
      Based on these results, the innate immune response patterns (particularly IFN-alpha, 
      TNF-alpha and IL-12) to specific PRRSV strains in PAMs might reflect those elicited 
      by the same viruses in pigs.
FAU - Shabir, Nadeem
AU  - Shabir N
AD  - College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan, 
      Jeonbuk, Korea.
AD  - Division of Animal Biotechnology, Faculty of Veterinary Sciences and Animal 
      Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of 
      Kashmir, Srinagar, India.
FAU - Khatun, Amina
AU  - Khatun A
AD  - College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan, 
      Jeonbuk, Korea.
FAU - Nazki, Salik
AU  - Nazki S
AD  - College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan, 
      Jeonbuk, Korea.
FAU - Gu, Suna
AU  - Gu S
AD  - College of Environmental & Biosource Science, Division of Biotechnology, Chonbuk 
      National University, Iksan, South Korea.
FAU - Lee, Sang-Myoung
AU  - Lee SM
AD  - College of Environmental & Biosource Science, Division of Biotechnology, Chonbuk 
      National University, Iksan, South Korea.
FAU - Hur, Tai-Young
AU  - Hur TY
AD  - Dairy Science Division, National Institute of Animal Science, Rural Development 
      Administration, Cheonan, 31000, South Korea.
FAU - Yang, Myoun-Sik
AU  - Yang MS
AD  - College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan, 
      Jeonbuk, Korea.
FAU - Kim, Bumseok
AU  - Kim B
AD  - College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan, 
      Jeonbuk, Korea.
FAU - Kim, Won-Il
AU  - Kim WI
AUID- ORCID: 0000-0002-0465-0794
AD  - College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan, 
      Jeonbuk, Korea. kwi0621@jbnu.ac.kr.
LA  - eng
GR  - 315029-3/Technology Development Program for Bio-industry/
GR  - PJ012612/Cooperative Research Program for Agriculture Science & Technology 
      Development/
PT  - Journal Article
DEP - 20181204
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Cytokines)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/blood
MH  - Immunity, Innate/immunology
MH  - Interferons/blood
MH  - Leukocytes, Mononuclear/immunology/virology
MH  - Macrophages, Alveolar/*immunology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - Swine
PMC - PMC6278023
OTO - NOTNLM
OT  - Alveolar macrophages
OT  - Flow cytometry
OT  - Immune response
OT  - PRRSV
OT  - Peripheral blood mononuclear cells
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: The animal experiment protocol was 
      approved by the Chonbuk National University Institutional Animal Care and Use 
      Committee (Approval Number: 2012-0025) and performed in accordance with the 
      guidelines and regulations of the committee. CONSENT FOR PUBLICATION: Not 
      applicable. COMPETING INTERESTS: The authors declare that they have no competing 
      interests. PUBLISHER'S NOTE: Springer Nature remains neutral with regard to 
      jurisdictional claims in published maps and institutional affiliations.
EDAT- 2018/12/05 06:00
MHDA- 2019/01/24 06:00
PMCR- 2018/12/04
CRDT- 2018/12/05 06:00
PHST- 2018/03/26 00:00 [received]
PHST- 2018/10/29 00:00 [accepted]
PHST- 2018/12/05 06:00 [entrez]
PHST- 2018/12/05 06:00 [pubmed]
PHST- 2019/01/24 06:00 [medline]
PHST- 2018/12/04 00:00 [pmc-release]
AID - 10.1186/s12917-018-1675-x [pii]
AID - 1675 [pii]
AID - 10.1186/s12917-018-1675-x [doi]
PST - epublish
SO  - BMC Vet Res. 2018 Dec 4;14(1):380. doi: 10.1186/s12917-018-1675-x.