PMID- 30511246
OWN - NLM
STAT- MEDLINE
DCOM- 20200608
LR  - 20211103
IS  - 1432-0932 (Electronic)
IS  - 0940-6719 (Linking)
VI  - 28
IP  - 4
DP  - 2019 Apr
TI  - A mouse model for the study of transplanted bone marrow mesenchymal stem cell 
      survival and proliferation in lumbar spinal fusion.
PG  - 710-718
LID - 10.1007/s00586-018-5839-y [doi]
AB  - PURPOSE: Bone marrow aspirate has been successfully used alongside a variety of 
      grafting materials to clinically augment spinal fusion. However, little is known 
      about the fate of these transplanted cells. Herein, we develop a novel murine 
      model for the in vivo monitoring of implanted bone marrow cells (BMCs) following 
      spinal fusion. METHODS: A clinical-grade scaffold was implanted into 
      immune-intact mice undergoing spinal fusion with or without freshly isolated BMCs 
      from either transgenic mice which constitutively express the firefly luciferase 
      gene or syngeneic controls. The in vivo survival, distribution and proliferation 
      of these luciferase-expressing cells was monitored via bioluminescence imaging 
      over a period of 8 weeks and confirmed via immunohistochemistry. MicroCT imaging 
      was performed 8 weeks to assess fusion. RESULTS: Bioluminescence imaging 
      indicated transplanted cell survival and proliferation over the first 2 weeks, 
      followed by a decrease in cell numbers, with transplanted cell survival still 
      evident at the end of the study. New bone formation and increased fusion mass 
      volume were observed in mice implanted with cell-seeded scaffolds. CONCLUSIONS: 
      By enabling the tracking of transplanted bone marrow-derived cells during spinal 
      fusion in vivo, this mouse model will be integral to developing a deeper 
      understanding of the biological processes underlying spinal fusion in future 
      studies. These slides can be retrieved under Electronic Supplementary Material.
FAU - Lina, Ioan A
AU  - Lina IA
AD  - Department of Otolaryngology, The Johns Hopkins University School of Medicine, 
      Baltimore, MD, USA.
FAU - Ishida, Wataru
AU  - Ishida W
AD  - Department of Neurosurgery, The Johns Hopkins University School of Medicine, 1550 
      Orleans St, Rm 2M-51, Baltimore, MD, 21287, USA.
FAU - Liauw, Jason A
AU  - Liauw JA
AD  - Department of Neurosurgery, The Johns Hopkins University School of Medicine, 1550 
      Orleans St, Rm 2M-51, Baltimore, MD, 21287, USA.
FAU - Lo, Sheng-Fu L
AU  - Lo SL
AD  - Department of Neurosurgery, The Johns Hopkins University School of Medicine, 1550 
      Orleans St, Rm 2M-51, Baltimore, MD, 21287, USA.
FAU - Elder, Benjamin D
AU  - Elder BD
AD  - Department of Neurological Surgery, Mayo Clinic School of Medicine, Rochester, 
      MN, USA.
FAU - Perdomo-Pantoja, Alexander
AU  - Perdomo-Pantoja A
AD  - Department of Neurosurgery, The Johns Hopkins University School of Medicine, 1550 
      Orleans St, Rm 2M-51, Baltimore, MD, 21287, USA.
FAU - Theodros, Debebe
AU  - Theodros D
AD  - Department of Neurosurgery, The Johns Hopkins University School of Medicine, 1550 
      Orleans St, Rm 2M-51, Baltimore, MD, 21287, USA.
FAU - Witham, Timothy F
AU  - Witham TF
AD  - Department of Neurosurgery, The Johns Hopkins University School of Medicine, 1550 
      Orleans St, Rm 2M-51, Baltimore, MD, 21287, USA.
FAU - Holmes, Christina
AU  - Holmes C
AUID- ORCID: 0000-0003-0736-0503
AD  - Department of Neurosurgery, The Johns Hopkins University School of Medicine, 1550 
      Orleans St, Rm 2M-51, Baltimore, MD, 21287, USA. christinaa.holmes@gmail.com.
LA  - eng
GR  - S-16-59H/AO Foundation/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20181203
PL  - Germany
TA  - Eur Spine J
JT  - European spine journal : official publication of the European Spine Society, the 
      European Spinal Deformity Society, and the European Section of the Cervical Spine 
      Research Society
JID - 9301980
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/methods
MH  - Cell Proliferation
MH  - Cell Survival
MH  - *Disease Models, Animal
MH  - Female
MH  - Lumbar Vertebrae/diagnostic imaging/pathology/*surgery
MH  - Luminescent Measurements/methods
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice, Transgenic
MH  - Spinal Fusion/*methods
MH  - Tissue Scaffolds
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - Bone marrow
OT  - Luciferase
OT  - Mesenchymal stem cell
OT  - Mouse model
OT  - Spinal fusion
EDAT- 2018/12/05 06:00
MHDA- 2020/06/09 06:00
CRDT- 2018/12/05 06:00
PHST- 2018/04/16 00:00 [received]
PHST- 2018/11/25 00:00 [accepted]
PHST- 2018/10/21 00:00 [revised]
PHST- 2018/12/05 06:00 [pubmed]
PHST- 2020/06/09 06:00 [medline]
PHST- 2018/12/05 06:00 [entrez]
AID - 10.1007/s00586-018-5839-y [pii]
AID - 10.1007/s00586-018-5839-y [doi]
PST - ppublish
SO  - Eur Spine J. 2019 Apr;28(4):710-718. doi: 10.1007/s00586-018-5839-y. Epub 2018 
      Dec 3.