PMID- 30513452
OWN - NLM
STAT- MEDLINE
DCOM- 20190430
LR  - 20190430
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 105
DP  - 2019 Jan
TI  - Nrf2/ARE pathway inhibits inflammatory infiltration by macrophage in rats with 
      autoimmune myositis.
PG  - 165-172
LID - S0161-5890(18)30578-9 [pii]
LID - 10.1016/j.molimm.2018.11.014 [doi]
AB  - BACKGROUND: Idiopathic inflammatory myopathies (IIM) are a group of autoimmune 
      diseases characterized by muscle disorders. We conducted this study to detect 
      whether NF-E2-related factor 2 (Nrf2) pathway inhibit inflammatory infiltration 
      by macrophage in experimental autoimmune myositis (EAM) rat model. METHODS: CD163 
      levels were examined by immunohistochemistry (IHC), while serum creatine kinase 
      (CK), reactive oxygen species (ROS), and serum monocyte chemoattractant protein-1 
      (MCP-1), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) levels were 
      determined by enzyme linked immunosorbnent assay (ELISA), both in IIM patients 
      and EAM rat. We also detected MCP-1, TNF-alpha, IL-6, and Nrf2 levels by Realtime 
      quantitative PCR (RT-PCR) in patients' muscles, and MCP-1, TNF-alpha, IL-6, and Nrf2, 
      HO-1, NQO-1 levels by RT-PCR and Western blot in EAM rats' muscles. EAM 
      macrophages were separated, and Nrf2 over-expression macrophages were 
      constructed. ROS level and cell migration were detected by flow cytometer and 
      transwell assay respectively. Then, levels of MCP-1, TNF-alpha, IL-6, Nrf2, Heme 
      oxygenase-1 (HO-1) and NAD(P)H: quinine oxidoreductase 1 (NQO-1) were detected by 
      RT-PCR and Western blot. RESULTS: Results showed that EAM rats were 
      histopathologically inflammatory cell infiltration. Levels of CD163, serum CK and 
      ROS, serum/muscle MCP-1, TNF-alpha and IL-6 increased and muscle Nrf2 level decreased 
      in IIM patients and EAM rats. Cell migration ability and levels of ROS, MCP-1, 
      TNF-alpha, IL-6, and plasma Nrf2 were down-regulated, and total/nucleus Nrf2, HO-1, 
      NQO-1 were up-regulated notably when Nrf2 over-expressed. CONCLUSION: Nrf2 
      inhibited EAM macrophage infiltration by activating Nrf2/ARE pathway which could 
      induce ROS degradation and inhibit pro-inflammatory factors expression.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Liu, Yutao
AU  - Liu Y
AD  - Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 
      China.
FAU - Gao, Yuan
AU  - Gao Y
AD  - Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 
      China.
FAU - Yang, Jing
AU  - Yang J
AD  - Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 
      China.
FAU - Shi, Changhe
AU  - Shi C
AD  - Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 
      China.
FAU - Wang, Yanlin
AU  - Wang Y
AD  - Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 
      China.
FAU - Xu, Yuming
AU  - Xu Y
AD  - Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 
      China. Electronic address: xuyuming@zzu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181202
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Cytokines)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, rat)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Hmox1 protein, rat)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (NQO1 protein, rat)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Cell Movement/*immunology
MH  - Cytokines/immunology
MH  - Female
MH  - Gene Expression Regulation/immunology
MH  - Heme Oxygenase (Decyclizing)/immunology
MH  - Humans
MH  - Macrophages, Peritoneal/*immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - NAD(P)H Dehydrogenase (Quinone)/immunology
MH  - NF-E2-Related Factor 2/*immunology
MH  - Nervous System Autoimmune Disease, Experimental/*immunology/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/*immunology
OTO - NOTNLM
OT  - Autoimmune myositis
OT  - IIM
OT  - Inflammatory infiltration
OT  - Macrophage
OT  - Nrf2/ARE pathway
OT  - PM/DM
EDAT- 2018/12/05 06:00
MHDA- 2019/05/01 06:00
CRDT- 2018/12/05 06:00
PHST- 2018/07/17 00:00 [received]
PHST- 2018/11/01 00:00 [revised]
PHST- 2018/11/20 00:00 [accepted]
PHST- 2018/12/05 06:00 [pubmed]
PHST- 2019/05/01 06:00 [medline]
PHST- 2018/12/05 06:00 [entrez]
AID - S0161-5890(18)30578-9 [pii]
AID - 10.1016/j.molimm.2018.11.014 [doi]
PST - ppublish
SO  - Mol Immunol. 2019 Jan;105:165-172. doi: 10.1016/j.molimm.2018.11.014. Epub 2018 
      Dec 2.