PMID- 30513512
OWN - NLM
STAT- MEDLINE
DCOM- 20190121
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 51
IP  - 4
DP  - 2018
TI  - miRNA-544a Regulates the Inflammation of Spinal Cord Injury by Inhibiting the 
      Expression of NEUROD4.
PG  - 1921-1931
LID - 10.1159/000495717 [doi]
AB  - BACKGROUND/AIMS: To explore the potential role of miR-544a in spinal cord injury 
      and the possible mechanism involved. METHODS: We established a mouse model with 
      spinal cord injury to examine the changes in grip force recovery of the forelimb 
      or the posterior limb of the mouse. Microarray was performed to achieve 
      differentiated miRNAs in the mice. The expressions of miR-544a, MCP-1, IL36B and 
      IL17B after spinal cord injury were detected by qRT-PCR. Subsequently, miR-544a 
      was overexpressed to observe changes in inflammation and grip strength after 
      spinal cord injury. Target gene of miR-544a was then predicted using 
      bioinformatics technology. Finally, dual luciferase reporter gene assay was used 
      to verify the binding of miR-544a to its target gene. RESULTS: Using mice models 
      with spinal cord injury, we found that the strength of their four limbs began to 
      recover 7 days after injury. The results of microarray and qRT-PCR confirmed that 
      mir-544a level in mice with spinal cord injury decreased with increase of injury 
      time, while the levels of inflammatory genes MCP-1 (monocyte chemoattractant 
      protein-1), IL1 (interleukin-1) and TNF-alpha (tumor necrosis factor alpha) IL36B 
      (interleukin-36 beta) and IL17B (interleukin-17 beta) were significantly 
      increased. However, overexpression of miR-544a in the mice significantly reduced 
      the level of inflammation and restored their grip strength in their four limbs. 
      Finally, we found that miR-544a can bind to the NEUROD4 (Neurogenic 
      differentiation 4) 3'UTR (Untranslated Region) region through bioinformatics 
      website prediction, which was further confirmed by dual luciferase reporter 
      assay. NEUROD4 level was significantly reduced following the overexpression of 
      miR-544a. CONCLUSION: The expression of miR-544a was significantly decreased 
      after spinal cord injury. High expression of miR-544a could alleviate the 
      inflammation caused by spinal cord injury and promote the recovery of spinal cord 
      via the inhibition of NEUROD4.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Yang, Lei
AU  - Yang L
AD  - Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Ge, Dawei
AU  - Ge D
AD  - Department of Orthopaedics, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Chen, Xi
AU  - Chen X
AD  - Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Jiang, Chunzhi
AU  - Jiang C
AD  - Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Zheng, Shengnai
AU  - Zheng S
AD  - Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, Chinaleiyangspine@163.com.
LA  - eng
PT  - Journal Article
DEP - 20181204
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (MIRN-544 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurod4 protein, mouse)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/*genetics/immunology
MH  - *Down-Regulation
MH  - Female
MH  - Inflammation/*genetics/immunology/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics/immunology
MH  - Nerve Tissue Proteins/*genetics/immunology
MH  - Spinal Cord/immunology/pathology
MH  - Spinal Cord Injuries/*genetics/immunology/pathology
MH  - Up-Regulation
OTO - NOTNLM
OT  - Inflammation
OT  - NEUROD4
OT  - Spinal cord injury
OT  - miR-544a
EDAT- 2018/12/05 06:00
MHDA- 2019/01/22 06:00
CRDT- 2018/12/05 06:00
PHST- 2018/03/09 00:00 [received]
PHST- 2018/11/23 00:00 [accepted]
PHST- 2018/12/05 06:00 [pubmed]
PHST- 2019/01/22 06:00 [medline]
PHST- 2018/12/05 06:00 [entrez]
AID - 000495717 [pii]
AID - 10.1159/000495717 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;51(4):1921-1931. doi: 10.1159/000495717. Epub 2018 Dec 
      4.