PMID- 30514049
OWN - NLM
STAT- MEDLINE
DCOM- 20190507
LR  - 20190507
IS  - 1003-0034 (Print)
IS  - 1003-0034 (Linking)
VI  - 31
IP  - 11
DP  - 2018 Nov 25
TI  - [Neuroprotective effect of icariin on spinal cord injury in rats].
PG  - 1054-1060
LID - 10.3969/j.issn.1003-0034.2018.11.014 [doi]
AB  - OBJECTIVE: To study the neuroprotective effect of icariin on spinal cord injury 
      in rats. METHODS: A total 108 SPF male 3-month-old SD rats were divided into 
      experimental group, control group and sham operation group according to the 
      random number table. There were 36 rats in each group. In the control group and 
      the experimental group, the modified Allen's method was used to make the spinal 
      cord injury model. In the sham operation group, only the lamina was cut without 
      damaging the spinal cord. Immediately after operation, the experimental group was 
      given intragastric administration of icariin(100 mg/kg), the control group and 
      sham operation group were given an equal amount of normal saline by gavage, twice 
      a day. BBB score was used to assess the motor function of rats on 1, 2, 3 days 
      after operation. At 72 h after operation, the activity of myeloperoxidase (MPO) 
      was measured by spectrophotometry. Tumor necrosis factor-alpha(TNF-alpha), 
      interleukin-1beta(IL-1beta) levels was detected by enzyme-linked immunosorbent 
      assay(ELISA). MPO, TNF-alpha, IL-1beta expression were detected by immunohistochemical 
      staining. Malondialdehyde (MDA) content was detected by thiobarbituric acid 
      method. Superoxide dismutase (SOD) activity was measured by xanthine oxidase 
      method. TUNEL staining was used to detect the apoptosis and apoptosis index(AI) 
      was calculated. The histopathological changes of the spinal cord were observed 
      under a light microscope and the histopathological score was performed using 
      Sirin score method. RESULTS: BBB score in the control group and the experimental 
      group was significantly lower than that in the sham operation group at each 
      postoperative time point(P<0.05). BBB score in the experimental group was 
      significantly higher than that in the control group at 2 and 3 days after 
      operation (P<0.05). At 72 h after operation, the MPO activity and the levels of 
      TNF-alpha, IL-1beta in the control group and experimental group were significantly 
      higher than in the sham operation group (P<0.05), and the experimental group was 
      obviously higher than control group(P<0.05). The expressions of MPO, TNF-alpha, IL-1beta 
      in the control group and experimental group were significantly higher than in the 
      sham operation group (P<0.05), and the experimental group was significantly lower 
      than of the control group (P<0.05). MDA content in the control group and the 
      experimental group was significantly higher than that in the sham operation 
      group, and the experimental group was significantly lower than that in the 
      control group (P<0.05). SOD activity in the control group and the experimental 
      group was significantly lower than that in the sham operation group, and the 
      experimental group was significantly higher than that in the control group 
      (P<0.05). The AI in the control group and the experimental group was 
      significantly higher than that in the sham operation group, and the experimental 
      group was significantly lower than that in the control group (P<0.05). The 
      histopathological score in the control group and the experimental group was 
      significantly higher than that in the sham operation group, and the experimental 
      group was significantly lower than that in the control group (P<0.05). 
      CONCLUSIONS: Icariin can inhibit inflammation, lipid peroxidation and apoptosis 
      after spinal cord injury, reduce histopathological damage of spinal cord, improve 
      the motor function, effectively protect spinal cord tissue, and has an obvious 
      neuroprotective effect.
CI  - Copyright(c) 2018 by the China Journal of Orthopaedics and Traumatology Press.
FAU - Ren, Xian-Sheng
AU  - Ren XS
AD  - Department of Orthopaedics, the Second Hospital of Jilin University, Changchun 
      130041, Jilin, China; antren@163.com.
FAU - Ding, Wei
AU  - Ding W
FAU - Yang, Xiao-Yu
AU  - Yang XY
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Gu Shang
JT  - Zhongguo gu shang = China journal of orthopaedics and traumatology
JID - 9815790
RN  - 0 (Flavonoids)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - VNM47R2QSQ (icariin)
SB  - IM
MH  - Animals
MH  - Flavonoids
MH  - Male
MH  - *Neuroprotective Agents
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord
MH  - *Spinal Cord Injuries
MH  - Tumor Necrosis Factor-alpha
OTO - NOTNLM
OT  - Icariin
OT  - Neuroprotection
OT  - Rats
OT  - Spinal cord injury
COIS- The authors of this article and the planning committee members and staff have no 
      relevant financial relationships with commercial interests to disclose.
EDAT- 2018/12/06 06:00
MHDA- 2019/05/08 06:00
CRDT- 2018/12/06 06:00
PHST- 2018/05/08 00:00 [received]
PHST- 2018/12/06 06:00 [entrez]
PHST- 2018/12/06 06:00 [pubmed]
PHST- 2019/05/08 06:00 [medline]
AID - 10.3969/j.issn.1003-0034.2018.11.014 [doi]
PST - ppublish
SO  - Zhongguo Gu Shang. 2018 Nov 25;31(11):1054-1060. doi: 
      10.3969/j.issn.1003-0034.2018.11.014.