PMID- 30514289
OWN - NLM
STAT- MEDLINE
DCOM- 20190115
LR  - 20211204
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 18
IP  - 1
DP  - 2018 Dec 4
TI  - Glycolysis inhibition via mTOR suppression is a key step in cardamonin-induced 
      autophagy in SKOV3 cells.
PG  - 317
LID - 10.1186/s12906-018-2380-9 [doi]
LID - 317
AB  - BACKGROUND: Autophagy occurs in cells that undergoing nutrient deprivation. 
      Glycolysis rapidly supplies energy for the proliferation of cancer cells. 
      Cardamonin inhibits proliferation and enhances autophagy by mTORC1 suppression in 
      ovarian cancer cells. Here, we investigate the relationship between 
      cardamonin-triggered autophagy and glycolysis inhibition via mTORC1 suppression. 
      METHODS: Treated with indicated compounds, ATP content and the activity of 
      hexokinase (HK) and lactate dehydrogenase (LDH) were analyzed by the assay kits. 
      Autophagy was detected by monodansylcadaverin (MDC) staining. The relationship 
      between cardamonin-triggered autophagy and glycolysis inhibition via mTORC1 
      suppression was analyzed by Western blot. RESULTS: We found that cardamonin 
      inhibited the lactate secretion, ATP production, and the activity of HK and LDH. 
      The results demonstrated that cardamonin enhanced autophagy in SKOV3 cells, as 
      indicated by acidic compartments accumulation, microtubule-associated protein 1 
      Light Chain 3-II (LC3-II) and lysosome associated membrane protein 1 
      up-regulation. Our results showed that the activation of mTORC1 signaling and the 
      expression HK2 were reduced by cardamonin; whereas the phosphorylation of AMPK 
      (AMP-activated protein kinase) was increased. We also confirmed that the AMPK 
      inhibitor, Compound C, reversed cardamonin-induced upregulation of LC3-II. 
      CONCLUSION: These results suggest that cardamonin-induced autophagy is associated 
      with inhibition on glycolysis by down-regulating the activity of mTORC1 in 
      ovarian cancer cells.
FAU - Shi, Daohua
AU  - Shi D
AUID- ORCID: 0000-0002-3383-5640
AD  - Department of Pharmacy, Fujian Provincial Maternity and Children's Hospital, 
      Affiliated Hospital of Fujian Medical University, 18 Daoshan Road, Fuzhou, 
      350001, Fujian, China. shidh@yeah.net.
FAU - Zhao, Di
AU  - Zhao D
AD  - Department of Pharmacy, Fujian Provincial Maternity and Children's Hospital, 
      Affiliated Hospital of Fujian Medical University, 18 Daoshan Road, Fuzhou, 
      350001, Fujian, China.
FAU - Niu, Peiguang
AU  - Niu P
AD  - Department of Pharmacy, Fujian Provincial Maternity and Children's Hospital, 
      Affiliated Hospital of Fujian Medical University, 18 Daoshan Road, Fuzhou, 
      350001, Fujian, China.
FAU - Zhu, Yanting
AU  - Zhu Y
AD  - Department of Pharmacy, Fujian Provincial Maternity and Children's Hospital, 
      Affiliated Hospital of Fujian Medical University, 18 Daoshan Road, Fuzhou, 
      350001, Fujian, China.
FAU - Zhou, Jintuo
AU  - Zhou J
AD  - Department of Pharmacy, Fujian Provincial Maternity and Children's Hospital, 
      Affiliated Hospital of Fujian Medical University, 18 Daoshan Road, Fuzhou, 
      350001, Fujian, China.
FAU - Chen, Huajiao
AU  - Chen H
AD  - Department of Pharmacy, Fujian Provincial Maternity and Children's Hospital, 
      Affiliated Hospital of Fujian Medical University, 18 Daoshan Road, Fuzhou, 
      350001, Fujian, China.
LA  - eng
GR  - 2016J01492/Natural Science Foundation of Fujian Province/
GR  - 2017J01234/Natural Science Foundation of Fujian Province/
GR  - 2017-1-18/Youth Foundation of Fujian Provincial Health and Family Planning 
      Commission/
GR  - 2017-1-19/Youth Foundation of Fujian Provincial Health and Family Planning 
      Commission/
GR  - 2016-CX-13/Innovative Medical Foundation of Fujian Provincial Health and Family 
      Planning Commission/
PT  - Journal Article
DEP - 20181204
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Chalcones)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - H8KP1OJ8JX (cardamonin)
SB  - IM
MH  - Autophagy/*drug effects
MH  - Cell Line, Tumor
MH  - Chalcones/*pharmacology
MH  - Glycolysis/*drug effects
MH  - Humans
MH  - TOR Serine-Threonine Kinases/*antagonists & inhibitors/*metabolism
PMC - PMC6278091
OTO - NOTNLM
OT  - AMPK
OT  - Autophagy
OT  - Cardamonin
OT  - Glycolysis
OT  - mTOR
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable. CONSENT FOR 
      PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they 
      have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral 
      with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2018/12/06 06:00
MHDA- 2019/01/16 06:00
PMCR- 2018/12/04
CRDT- 2018/12/06 06:00
PHST- 2018/07/04 00:00 [received]
PHST- 2018/11/20 00:00 [accepted]
PHST- 2018/12/06 06:00 [entrez]
PHST- 2018/12/06 06:00 [pubmed]
PHST- 2019/01/16 06:00 [medline]
PHST- 2018/12/04 00:00 [pmc-release]
AID - 10.1186/s12906-018-2380-9 [pii]
AID - 2380 [pii]
AID - 10.1186/s12906-018-2380-9 [doi]
PST - epublish
SO  - BMC Complement Altern Med. 2018 Dec 4;18(1):317. doi: 10.1186/s12906-018-2380-9.