PMID- 30514892
OWN - NLM
STAT- MEDLINE
DCOM- 20191017
LR  - 20231004
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Dec 4
TI  - Aspartic and Glutamic Acid Templated Peptides Conjugation on Plasma Modified 
      Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells: A 
      Comparative Study.
PG  - 17620
LID - 10.1038/s41598-018-36109-5 [doi]
LID - 17620
AB  - Optimization of nanofiber (NF) surface properties is critical to achieve an 
      adequate cellular response. Here, the impact of conjugation of biomimetic 
      aspartic acid (ASP) and glutamic acid (GLU) templated peptides with 
      poly(lactic-co-glycolic acid) (PLGA) electrospun NF on osteogenic differentiation 
      of human bone marrow-derived mesenchymal stem cells (hMSCs) was evaluated. Cold 
      atmospheric plasma (CAP) was used to functionalize the NF surface and thus to 
      mediate the conjugation. The influence of the CAP treatment following with 
      peptide conjugation to the NF surface was assessed using water contact angle 
      measurements, Fourier-Transform Infrared Spectroscopy (FTIR) and X-ray 
      Photoelectron Spectroscopy (XPS). The effect of CAP treatment on morphology of NF 
      was also checked using Scanning Electron Microscopy (SEM). Both the 
      hydrophilicity of NF and the number of the carboxyl (-COOH) groups on the surface 
      increased with respect to CAP treatment. Results demonstrated that CAP treatment 
      significantly enhanced peptide conjugation on the surface of NF. Osteogenic 
      differentiation results indicated that conjugating of biomimetic ASP templated 
      peptides sharply increased alkaline phosphatase (ALP) activity, calcium content, 
      and expression of key osteogenic markers of collagen type I (Col-I), osteocalcin 
      (OC), and osteopontin (OP) compared to GLU conjugated (GLU-pNF) and CAP treated 
      NF (pNF). It was further depicted that ASP sequences are the major fragments that 
      influence the mineralization and osteogenic differentiation in non-collagenous 
      proteins of bone extracellular matrix.
FAU - Onak, Gunnur
AU  - Onak G
AD  - Department of Biomedical Engineering, Izmir Katip Celebi University, Izmir, 
      35620, Turkey.
FAU - Sen, Mustafa
AU  - Sen M
AD  - Department of Biomedical Engineering, Izmir Katip Celebi University, Izmir, 
      35620, Turkey.
FAU - Horzum, Nesrin
AU  - Horzum N
AD  - Department of Engineering Sciences, Izmir Katip Celebi University, Izmir, 35620, 
      Turkey.
FAU - Ercan, Utku Kursat
AU  - Ercan UK
AD  - Department of Biomedical Engineering, Izmir Katip Celebi University, Izmir, 
      35620, Turkey.
FAU - Yarali, Ziysan Buse
AU  - Yarali ZB
AUID- ORCID: 0000-0002-9371-6424
AD  - Department of Biomedical Engineering, Izmir Katip Celebi University, Izmir, 
      35620, Turkey.
FAU - Garipcan, Bora
AU  - Garipcan B
AUID- ORCID: 0000-0002-1773-5607
AD  - Institute of Biomedical Engineering, Bogazici University, 34684, Istanbul, 
      Turkey.
FAU - Karaman, Ozan
AU  - Karaman O
AUID- ORCID: 0000-0002-4175-4402
AD  - Department of Biomedical Engineering, Izmir Katip Celebi University, Izmir, 
      35620, Turkey. ozan.karaman@ikc.edu.tr.
AD  - Bonegraft Biomaterials Co., Ege University Technopolis, 35100, Bornova, Izmir, 
      Turkey. ozan.karaman@ikc.edu.tr.
LA  - eng
GR  - 117S429/Türkiye Bilimsel ve Teknolojik Araştirma Kurumu 
      (Scientific and Technological Research Council of Turkey)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181204
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Peptides)
RN  - 0 (Plasma Gases)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - Aspartic Acid/*metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Glutamic Acid/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*drug effects/*physiology
MH  - Microscopy, Electron, Scanning
MH  - Nanofibers/*chemistry
MH  - Osteogenesis/drug effects
MH  - Peptides/*metabolism
MH  - Plasma Gases
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Surface Tension
PMC - PMC6279782
COIS- The authors declare no competing interests.
EDAT- 2018/12/06 06:00
MHDA- 2019/10/18 06:00
PMCR- 2018/12/04
CRDT- 2018/12/06 06:00
PHST- 2018/06/20 00:00 [received]
PHST- 2018/11/14 00:00 [accepted]
PHST- 2018/12/06 06:00 [entrez]
PHST- 2018/12/06 06:00 [pubmed]
PHST- 2019/10/18 06:00 [medline]
PHST- 2018/12/04 00:00 [pmc-release]
AID - 10.1038/s41598-018-36109-5 [pii]
AID - 36109 [pii]
AID - 10.1038/s41598-018-36109-5 [doi]
PST - epublish
SO  - Sci Rep. 2018 Dec 4;8(1):17620. doi: 10.1038/s41598-018-36109-5.