PMID- 30515676
OWN - NLM
STAT- MEDLINE
DCOM- 20190603
LR  - 20200225
IS  - 1936-0541 (Electronic)
IS  - 1936-0533 (Print)
IS  - 1936-0533 (Linking)
VI  - 13
IP  - 1
DP  - 2019 Jan
TI  - Precision-cut liver slices: a versatile tool to advance liver research.
PG  - 51-57
LID - 10.1007/s12072-018-9913-7 [doi]
AB  - Human precision-cut liver slices represent a robust and versatile ex vivo model 
      which retains the complex and multi-cellular histoarchitecture of the hepatic 
      environment. As such, they represent an ideal model to investigate the mechanisms 
      of liver injury and for the identification of novel therapeutic targets. 
      Schematic overview to highlight the utility of precision-cut liver slices as a 
      relevant and versatile ex-vivo model of liver disease. Top panel; Precision cut 
      liver slices (PCLS) exposed to ethanol develop mega-mitochondria, a classical 
      hallmark of Alcoholic Liver Disease (ALD). Right panel; PCLS from liver tumours 
      can be used as a model for liver cancer and can be used to investigate 
      cancer-immune cell interactions by co-culturing with matched immune cells. Bottom 
      panel; Exposure to a mixture of oleic and linoleic acids can simulate 
      Non-Alcoholic Fatty Liver Disease (NAFLD). Left panel; PCLS can be infected with 
      Hepatitis B and C virus and used as a model to study viral infection and 
      replication.
FAU - Palma, Elena
AU  - Palma E
AD  - Institute of Hepatology London, Foundation for Liver Research, London, UK.
AD  - Faculty of Life Sciences and Medicine, King's College London, London, UK.
FAU - Doornebal, Ewald Jan
AU  - Doornebal EJ
AD  - Institute of Hepatology London, Foundation for Liver Research, London, UK.
AD  - Faculty of Life Sciences and Medicine, King's College London, London, UK.
FAU - Chokshi, Shilpa
AU  - Chokshi S
AUID- ORCID: 0000-0003-1735-8538
AD  - Institute of Hepatology London, Foundation for Liver Research, London, UK. 
      s.chokshi@researchinliver.org.uk.
AD  - Faculty of Life Sciences and Medicine, King's College London, London, UK. 
      s.chokshi@researchinliver.org.uk.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20181204
PL  - United States
TA  - Hepatol Int
JT  - Hepatology international
JID - 101304009
SB  - IM
MH  - Humans
MH  - Liver/*pathology
MH  - Liver Diseases/*pathology
MH  - Models, Biological
MH  - Organ Culture Techniques
MH  - Specimen Handling/methods
MH  - Tumor Cells, Cultured
PMC - PMC6513823
OTO - NOTNLM
OT  - Liver disease model
OT  - Liver slices
OT  - Organotypic system
COIS- This article does not contain any direct studies with human or animal subjects.
EDAT- 2018/12/06 06:00
MHDA- 2019/06/04 06:00
PMCR- 2018/12/04
CRDT- 2018/12/06 06:00
PHST- 2018/06/19 00:00 [received]
PHST- 2018/11/09 00:00 [accepted]
PHST- 2018/12/06 06:00 [pubmed]
PHST- 2019/06/04 06:00 [medline]
PHST- 2018/12/06 06:00 [entrez]
PHST- 2018/12/04 00:00 [pmc-release]
AID - 10.1007/s12072-018-9913-7 [pii]
AID - 9913 [pii]
AID - 10.1007/s12072-018-9913-7 [doi]
PST - ppublish
SO  - Hepatol Int. 2019 Jan;13(1):51-57. doi: 10.1007/s12072-018-9913-7. Epub 2018 Dec 
      4.