PMID- 30516855 OWN - NLM STAT- MEDLINE DCOM- 20190207 LR - 20210109 IS - 1939-1676 (Electronic) IS - 0891-6640 (Print) IS - 0891-6640 (Linking) VI - 33 IP - 1 DP - 2019 Jan TI - Clinical, histopathologic, cystoscopic, and fluorescence in situ hybridization analysis of proliferative urethritis in 22 dogs. PG - 184-191 LID - 10.1111/jvim.15349 [doi] AB - BACKGROUND: Proliferative urethritis (PU) is a lower urinary tract disease of dogs characterized by frond-like lesions in the urethra. The etiology of PU is unknown, although an association with bacterial cystitis is reported. OBJECTIVES: Deep-seated bacterial cystitis is associated with PU, particularly in dogs with neutrophilic or granulomatous inflammation. ANIMALS: Twenty-two client-owned dogs with PU and 5 control dogs euthanized for non-urinary disease. METHODS: In retrospective analysis, medical records of dogs with PU from 1986 to 2016 were reviewed. Signalment, clinical signs, cystoscopic findings, antimicrobial use, and results of urine, bladder, or urethral tissue cultures, if available, were recorded. Histopathology was reviewed and classified as lymphocytic-plasmacytic (LP), neutrophilic, LP-neutrophilic (LPN), granulomatous, or pleocellular. Eubacterial fluorescence in situ hybridization (FISH) was performed on 18 tissue samples (13 cases, 5 controls), with subsequent evaluation of bacterial species. RESULTS: Of the 22 dogs, 9 had LP urethritis, 6 had LPN, 4 had pleocellular, and 3 had neutrophilic urethritis. Of note, 7 of 13 PU samples were FISH+ for adherent or invasive bacteria; 1 of 5 controls were FISH+ for adherent bacteria. Five dogs had negative urine and tissue cultures when FISH was positive. There was no association detected between the type of urethral inflammation and the results of urine and tissue culture or FISH. CONCLUSIONS AND CLINICAL IMPORTANCE: The type of inflammation varied widely in these 22 PU cases. Deep-seated bacterial urethritis could be contributing to the inflammatory process in some dogs, regardless of the inflammation type. Urine and tissue cultures likely underestimate bacterial colonization of the urethra in dogs. CI - (c) 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine. FAU - Borys, Moria A AU - Borys MA AUID- ORCID: 0000-0001-5058-1243 AD - Veterinary Medical Teaching Hospital, University of California-Davis School of Veterinary Medicine, California. FAU - Hulsebosch, Sean E AU - Hulsebosch SE AD - Department of Medicine and Epidemiology, Veterinary Medical Teaching Hospital, University of California-Davis School of Veterinary Medicine, California. FAU - Mohr, F Charles AU - Mohr FC AD - Department of Anatomic Pathology, Veterinary Medical Teaching Hospital, University of California-Davis School of Veterinary Medicine, California. FAU - Watson, Katherine D AU - Watson KD AD - Department of Anatomic Pathology, Veterinary Medical Teaching Hospital, University of California-Davis School of Veterinary Medicine, California. FAU - Sykes, Jane E AU - Sykes JE AD - Department of Medicine and Epidemiology, Veterinary Medical Teaching Hospital, University of California-Davis School of Veterinary Medicine, California. FAU - Simpson, Kenneth W AU - Simpson KW AD - Department of Clinical Sciences, Cornell University College of Veterinary Medicine, New York. FAU - Westropp, Jodi L AU - Westropp JL AUID- ORCID: 0000-0003-1287-3979 AD - Department of Medicine and Epidemiology, Veterinary Medical Teaching Hospital, University of California-Davis School of Veterinary Medicine, California. LA - eng GR - 2016-32-R/Center for Companion Animal Health, School of Veterinary Medicine, University of California-Davis/ PT - Journal Article DEP - 20181205 PL - United States TA - J Vet Intern Med JT - Journal of veterinary internal medicine JID - 8708660 SB - IM MH - Animals MH - Cystoscopy/veterinary MH - Dog Diseases/*pathology MH - Dogs MH - Female MH - In Situ Hybridization, Fluorescence/veterinary MH - Male MH - Retrospective Studies MH - Urethra/pathology MH - Urethritis/pathology/*veterinary MH - Urinary Bladder/pathology PMC - PMC6335574 OTO - NOTNLM OT - granulomatous disease OT - lower urinary tract disease OT - urethral obstruction OT - urinary tract infection EDAT- 2018/12/06 06:00 MHDA- 2019/02/08 06:00 PMCR- 2019/01/01 CRDT- 2018/12/06 06:00 PHST- 2018/03/07 00:00 [received] PHST- 2018/10/05 00:00 [accepted] PHST- 2018/12/06 06:00 [pubmed] PHST- 2019/02/08 06:00 [medline] PHST- 2018/12/06 06:00 [entrez] PHST- 2019/01/01 00:00 [pmc-release] AID - JVIM15349 [pii] AID - 10.1111/jvim.15349 [doi] PST - ppublish SO - J Vet Intern Med. 2019 Jan;33(1):184-191. doi: 10.1111/jvim.15349. Epub 2018 Dec 5.