PMID- 30517024
OWN - NLM
STAT- MEDLINE
DCOM- 20191230
LR  - 20200930
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 316
IP  - 3
DP  - 2019 Mar 1
TI  - The highly efficient powerhouse in the Wistar audiogenic rat, an epileptic rat 
      strain.
PG  - R243-R254
LID - 10.1152/ajpregu.00254.2018 [doi]
AB  - The Wistar audiogenic rat (WAR) is an animal model of tonic-clonic epileptic 
      seizures, developed after genetic selection by sister x brother inbreeding of 
      Wistar rats susceptible to sound stimuli. Although metabolic changes have been 
      described in this strain, nothing is known about its mitochondrial metabolism. 
      Here, we addressed mitochondrial aspects of oxidative phosphorylation, oxidative 
      stress, biogenesis, and dynamics in liver, skeletal muscle, and heart of male 
      WARs and correlating them with physiological aspects of body metabolism. The 
      results showed higher mitochondrial content, respiration rates in phosphorylation 
      and noncoupled states, and H(2)O(2) production in WARs. Liver presented higher 
      content of peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC1alpha) 
      and mammalian target of rapamycin, proteins related to mitochondrial biogenesis. 
      In agreement, isolated liver mitochondria from WARs showed higher respiration 
      rates in phosphorylation state and ADP-to-O ratio, as well as higher content of 
      proteins related to electron transport chain ATP synthase, TCA cycle, and 
      mitochondrial fusion and fission compared with their Wistar counterparts. 
      Mitochondria with higher area and perimeter and more variable shapes were found 
      in liver and soleus from WARs in addition to lower reduced-to-oxidized 
      glutathione ratio. In vivo, WARs demonstrated lower body mass and energy 
      expenditure but higher food and water intake and amino acid oxidation. When 
      exposed to a running test, WARs reached higher speed and resisted for a longer 
      time and distance than their Wistar controls. In conclusion, the WAR strain has 
      mitochondrial changes in liver, skeletal muscle, and heart that improve its 
      mitochondrial capacity of ATP production, making it an excellent rat model to 
      study PGC1alpha overexpression and mitochondrial function in different physiological 
      conditions or facing pathological challenges.
FAU - Dechandt, Carlos Roberto Porto
AU  - Dechandt CRP
AD  - Departamento de Fisica e Quimica, Faculdade de Ciencias Farmaceuticas de Ribeirao 
      Preto, Universidade de Sao Paulo , Ribeirao Preto, Sao Paulo , Brazil.
FAU - Vicentini, Tatiane M
AU  - Vicentini TM
AD  - Departamento de Fisica e Quimica, Faculdade de Ciencias Farmaceuticas de Ribeirao 
      Preto, Universidade de Sao Paulo , Ribeirao Preto, Sao Paulo , Brazil.
FAU - Lanfredi, Guilherme Pauperio
AU  - Lanfredi GP
AD  - Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, 
      Universidade de Sao Paulo , Ribeirao Preto, Sao Paulo , Brazil.
FAU - Silva-Jr, Rui M P
AU  - Silva-Jr RMP
AD  - Departamento de Biologia Celular e Molecular, Faculdade de Medicina de Ribeirao 
      Preto, Universidade de Sao Paulo , Ribeirao Preto, Sao Paulo , Brazil.
FAU - Espreafico, Enilza Maria
AU  - Espreafico EM
AD  - Departamento de Biologia Celular e Molecular, Faculdade de Medicina de Ribeirao 
      Preto, Universidade de Sao Paulo , Ribeirao Preto, Sao Paulo , Brazil.
FAU - de Oliveira, Jose A Cortes
AU  - de Oliveira JAC
AD  - Departamento de Fisiologia, Faculdade de Medicina de Ribeirao Preto, Universidade 
      de Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil.
FAU - Faca, Vitor Marcel
AU  - Faca VM
AD  - Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, 
      Universidade de Sao Paulo , Ribeirao Preto, Sao Paulo , Brazil.
FAU - Garcia-Cairasco, Norberto
AU  - Garcia-Cairasco N
AD  - Departamento de Fisiologia, Faculdade de Medicina de Ribeirao Preto, Universidade 
      de Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil.
FAU - Alberici, Luciane Carla
AU  - Alberici LC
AUID- ORCID: 0000-0002-7464-9385
AD  - Departamento de Fisica e Quimica, Faculdade de Ciencias Farmaceuticas de Ribeirao 
      Preto, Universidade de Sao Paulo , Ribeirao Preto, Sao Paulo , Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181205
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, rat)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Adenosine Triphosphate/biosynthesis
MH  - Animals
MH  - Body Weight
MH  - Energy Metabolism
MH  - Epilepsy, Reflex/*metabolism
MH  - Hydrogen Peroxide/metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Mitochondria, Liver/metabolism
MH  - Muscle, Skeletal/metabolism
MH  - Myocardium/metabolism
MH  - Oxidative Phosphorylation
MH  - Oxidative Stress
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 
      1-alpha/biosynthesis/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Running
OTO - NOTNLM
OT  - Wistar audiogenic rat strain
OT  - body metabolism
OT  - experimental epilepsy model
OT  - liver
OT  - mitochondria
OT  - mitochondrial dynamics
OT  - reactive oxygen species
EDAT- 2018/12/06 06:00
MHDA- 2019/12/31 06:00
CRDT- 2018/12/06 06:00
PHST- 2018/12/06 06:00 [pubmed]
PHST- 2019/12/31 06:00 [medline]
PHST- 2018/12/06 06:00 [entrez]
AID - 10.1152/ajpregu.00254.2018 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2019 Mar 1;316(3):R243-R254. doi: 
      10.1152/ajpregu.00254.2018. Epub 2018 Dec 5.