PMID- 30519536
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2222-0682 (Print)
IS  - 2222-0682 (Electronic)
IS  - 2222-0682 (Linking)
VI  - 8
IP  - 3
DP  - 2018 Nov 29
TI  - Precision medicine allergy immunoassay methods for assessing immunoglobulin E 
      sensitization to aeroallergen molecules.
PG  - 17-36
LID - 10.5662/wjm.v8.i3.17 [doi]
AB  - Molecular-based allergy diagnosis for the in vitro assessment of a patient 
      immunoglobulin E (IgE) sensitization profile at the molecular level uses allergen 
      molecules (also referred to as allergen components), which may be well-defined, 
      highly purified, natural allergen components or recombinant allergens. Modern 
      immunoassay methods used for the detection of specific IgE against aeroallergen 
      components are either singleplex (such as the fluorescence enzyme immunoassay 
      with capsulated cellulose polymer solid-phase coupled allergens, the 
      enzyme-enhanced chemiluminescence immunoassay and the reversed enzyme 
      allergosorbent test, with liquid-phase allergens), multiparameter (such as the 
      line blot immunoassay for defined partial allergen diagnostics with allergen 
      components coating membrane strips) or multiplex (such as the microarray-based 
      immunoassay on immuno solid-phase allergen chip, and the two new multiplex 
      nanotechnology-based immunoassays: the patient-friendly allergen nano-bead array, 
      and the macroarray nanotechnology-based immunoassay used as a molecular allergy 
      explorer). The precision medicine diagnostic work-up may be organized as an 
      integrated "U-shape" approach, with a "top-down" approach (from symptoms to 
      molecules) and a "bottom-up" approach (from molecules to clinical implications), 
      as needed in selected patients. The comprehensive and accurate IgE sensitization 
      molecular profiling, with identification of the relevant allergens, is indicated 
      within the framework of a detailed patient's clinical history to distinguish 
      genuine IgE sensitization from sensitization due to cross-reactivity (especially 
      in polysensitized patients), to assess unclear symptoms and unsatisfactory 
      response to treatment, to reveal unexpected sensitizations, and to improve 
      assessment of severity and risk aspects in some patients. Practical approaches, 
      such as anamnesis molecular thinking, laboratory molecular thinking and 
      postmolecular anamnesis, are sometimes applied. The component-resolved diagnosis 
      of the specific IgE repertoire has a key impact on optimal decisions making for 
      prophylactic and specific immunotherapeutic strategies tailored for the 
      individual patient.
FAU - Popescu, Florin-Dan
AU  - Popescu FD
AD  - Department of Allergology, "Carol Davila" University of Medicine and Pharmacy, 
      Bucharest 022441, Romania.
FAU - Vieru, Mariana
AU  - Vieru M
AD  - Department of Allergology, "Carol Davila" University of Medicine and Pharmacy, 
      Bucharest 022441, Romania.
LA  - eng
PT  - Editorial
DEP - 20181129
PL  - United States
TA  - World J Methodol
JT  - World journal of methodology
JID - 101628739
PMC - PMC6275558
OTO - NOTNLM
OT  - Aeroallergens
OT  - Immunoassays
OT  - Immunoglobulin E sensitization
OT  - Multiplex
OT  - Singleplex
COIS- Conflict-of-interest statement: The authors have no conflict of interest to 
      declare.
EDAT- 2018/12/07 06:00
MHDA- 2018/12/07 06:01
PMCR- 2018/11/29
CRDT- 2018/12/07 06:00
PHST- 2018/07/09 00:00 [received]
PHST- 2018/08/09 00:00 [revised]
PHST- 2018/10/09 00:00 [accepted]
PHST- 2018/12/07 06:00 [entrez]
PHST- 2018/12/07 06:00 [pubmed]
PHST- 2018/12/07 06:01 [medline]
PHST- 2018/11/29 00:00 [pmc-release]
AID - 10.5662/wjm.v8.i3.17 [doi]
PST - epublish
SO  - World J Methodol. 2018 Nov 29;8(3):17-36. doi: 10.5662/wjm.v8.i3.17. eCollection 
      2018 Nov 29.