PMID- 30520941
OWN - NLM
STAT- MEDLINE
DCOM- 20200518
LR  - 20220929
IS  - 1755-3245 (Electronic)
IS  - 0008-6363 (Print)
IS  - 0008-6363 (Linking)
VI  - 115
IP  - 6
DP  - 2019 May 1
TI  - Loss of CXCR4 on non-classical monocytes in participants of the Women's 
      Interagency HIV Study (WIHS) with subclinical atherosclerosis.
PG  - 1029-1040
LID - 10.1093/cvr/cvy292 [doi]
AB  - AIMS: To test whether human immunodeficiency virus (HIV) infection and 
      subclinical cardiovascular disease (sCVD) are associated with expression of CXCR4 
      and other surface markers on classical, intermediate, and non-classical monocytes 
      in women. METHODS AND RESULTS: sCVD was defined as presence of atherosclerotic 
      lesions in the carotid artery in 92 participants of the Women's Interagency HIV 
      Study (WIHS). Participants were stratified into four sets (n = 23 each) by HIV 
      and sCVD status (HIV-/sCVD-, HIV-/sCVD+, HIV+/sCVD-, and HIV+/sCVD+) matched by 
      age, race/ethnicity, and smoking status. Three subsets of monocytes were 
      determined from archived peripheral blood mononuclear cells. Flow cytometry was 
      used to count and phenotype surface markers. We tested for differences by HIV and 
      sCVD status accounting for multiple comparisons. We found no differences in 
      monocyte subset size among the four groups. Expression of seven surface markers 
      differed significantly across the three monocyte subsets. CXCR4 expression 
      [median fluorescence intensity (MFI)] in non-classical monocytes was highest 
      among HIV-/CVD- [628, interquartile range (IQR) (295-1389)], followed by 
      HIV+/CVD- [486, IQR (248-699)], HIV-/CVD+ (398, IQR (89-901)), and lowest in 
      HIV+/CVD+ women [226, IQR (73-519)), P = 0.006 in ANOVA. After accounting for 
      multiple comparison (Tukey) the difference between HIV-/CVD- vs. HIV+/CVD+ 
      remained significant with P = 0.005 (HIV-/CVD- vs. HIV+/CVD- P = 0.04, HIV-/CVD- 
      vs. HIV-/CVD+ P = 0.06, HIV+/CVD+ vs. HIV+/CVD- P = 0.88, HIV+/CVD+ vs. HIV-/CVD+ 
      P = 0.81, HIV+/CVD- vs. HIV-/CVD+, P = 0.99). All pairwise comparisons with 
      HIV-/CVD- were individually significant (P = 0.050 vs. HIV-/CVD+, P = 0.028 vs. 
      HIV+/CVD-, P = 0.009 vs. HIV+/CVD+). CXCR4 expression on non-classical monocytes 
      was significantly higher in CVD- (501.5, IQR (249.5-887.3)) vs. CVD+ (297, IQR 
      (81.75-626.8) individuals (P = 0.028, n = 46 per group). CXCR4 expression on 
      non-classical monocytes significantly correlated with cardiovascular and 
      HIV-related risk factors including systolic blood pressure, platelet and T cell 
      counts along with duration of antiretroviral therapy (P < 0.05). In regression 
      analyses, adjusted for education level, study site, and injection drug use, 
      presence of HIV infection and sCVD remained significantly associated with lower 
      CXCR4 expression on non-classical monocytes (P = 0.003), but did not differ in 
      classical or intermediate monocytes. CONCLUSION: CXCR4 expression in 
      non-classical monocytes was significantly lower among women with both HIV 
      infection and sCVD, suggesting a potential atheroprotective role of CXCR4 in 
      non-classical monocytes.
CI  - Published on behalf of the European Society of Cardiology. All rights reserved. (c) 
      The Author(s) 2018. For permissions, please email: journals.permissions@oup.com.
FAU - Mueller, Karin A L
AU  - Mueller KAL
AD  - La Jolla Institute of Immunology, Athena Circle Drive La Jolla, CA, USA.
AD  - Eberhard Karls University, Tuebingen University Hospital, Department of 
      Cardiology, Otfried-Mueller-Strasse 10, Tuebingen, Germany.
FAU - Hanna, David B
AU  - Hanna DB
AD  - Department of Epidemiology and Population Health, Albert Einstein College of 
      Medicine, 1300 Morris Park Avenue, Belfer 13th floor, Bronx, NY, USA.
FAU - Ehinger, Erik
AU  - Ehinger E
AD  - La Jolla Institute of Immunology, Athena Circle Drive La Jolla, CA, USA.
FAU - Xue, Xiaonan
AU  - Xue X
AD  - Department of Epidemiology and Population Health, Albert Einstein College of 
      Medicine, 1300 Morris Park Avenue, Belfer 13th floor, Bronx, NY, USA.
FAU - Baas, Livia
AU  - Baas L
AD  - La Jolla Institute of Immunology, Athena Circle Drive La Jolla, CA, USA.
AD  - Eberhard Karls University, Tuebingen University Hospital, Department of 
      Cardiology, Otfried-Mueller-Strasse 10, Tuebingen, Germany.
FAU - Gawaz, Meinrad P
AU  - Gawaz MP
AD  - Eberhard Karls University, Tuebingen University Hospital, Department of 
      Cardiology, Otfried-Mueller-Strasse 10, Tuebingen, Germany.
FAU - Geisler, Tobias
AU  - Geisler T
AD  - Eberhard Karls University, Tuebingen University Hospital, Department of 
      Cardiology, Otfried-Mueller-Strasse 10, Tuebingen, Germany.
FAU - Anastos, Kathryn
AU  - Anastos K
AD  - Department of Epidemiology and Population Health, Albert Einstein College of 
      Medicine, 1300 Morris Park Avenue, Belfer 13th floor, Bronx, NY, USA.
FAU - Cohen, Mardge H
AU  - Cohen MH
AD  - John H. Stroger, Jr. Hospital of Cook County, 1969 Ogden Ave, Chicago, IL, USA.
FAU - Gange, Stephen J
AU  - Gange SJ
AD  - Department of Epidemiology, Johns Hopkins University, 265 Garland Hall, 3400 
      North Charles Street, Baltimore, MD, USA.
FAU - Heath, Sonya L
AU  - Heath SL
AD  - Department of Medicine, University of Alabama at Birmingham, 908 20th Street 
      South, Birmingham, AL, USA.
FAU - Lazar, Jason M
AU  - Lazar JM
AD  - Department of Medicine, SUNY-Downstate Medical Center, 450 Clarkson Ave, 
      Brooklyn, NY, USA.
FAU - Liu, Chenglong
AU  - Liu C
AD  - Georgetown University Medical Center, Washington, DC, USA.
FAU - Mack, Wendy J
AU  - Mack WJ
AD  - Department of Preventive Medicine, University of Southern California, 2001 N Soto 
      St, Los Angeles, CA, USA.
FAU - Ofotokun, Igho
AU  - Ofotokun I
AD  - Division of Infectious Diseases, Department of Medicine, Emory University School 
      of Medicine, 49 Jesse Hill Jr. Drive, Atlanta, GA, USA.
FAU - Tien, Phyllis C
AU  - Tien PC
AD  - Department of Medicine, VAMC, Infectious Disease Section, 111W 4150 Clement St., 
      San Francisco, CA, USA.
FAU - Hodis, Howard N
AU  - Hodis HN
AD  - Department of Preventive Medicine, University of Southern California, 2001 N Soto 
      St, Los Angeles, CA, USA.
FAU - Landay, Alan L
AU  - Landay AL
AD  - Department of Internal Medicine, Rush University Medical Center, 1735 West 
      Harrison St, Chicago, IL, USA.
FAU - Kaplan, Robert C
AU  - Kaplan RC
AD  - Department of Epidemiology and Population Health, Albert Einstein College of 
      Medicine, 1300 Morris Park Avenue, Belfer 13th floor, Bronx, NY, USA.
AD  - Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, 
      WA, USA.
FAU - Ley, Klaus
AU  - Ley K
AD  - La Jolla Institute of Immunology, Athena Circle Drive La Jolla, CA, USA.
LA  - eng
GR  - P30 AI050409/AI/NIAID NIH HHS/United States
GR  - U01 AI103397/AI/NIAID NIH HHS/United States
GR  - U01 AI031834/AI/NIAID NIH HHS/United States
GR  - U01 AI035004/AI/NIAID NIH HHS/United States
GR  - P30 AI051519/AI/NIAID NIH HHS/United States
GR  - R01 HL095140/HL/NHLBI NIH HHS/United States
GR  - R01 HL083760/HL/NHLBI NIH HHS/United States
GR  - UL1 TR000454/TR/NCATS NIH HHS/United States
GR  - U01 AI103401/AI/NIAID NIH HHS/United States
GR  - UL1 TR000004/TR/NCATS NIH HHS/United States
GR  - U01 AI103408/AI/NIAID NIH HHS/United States
GR  - U01 AI034989/AI/NIAID NIH HHS/United States
GR  - R21 HL120394/HL/NHLBI NIH HHS/United States
GR  - R01 HL126543/HL/NHLBI NIH HHS/United States
GR  - U01 AI034994/AI/NIAID NIH HHS/United States
GR  - R21 AG059505/AG/NIA NIH HHS/United States
GR  - U01 AI103390/AI/NIAID NIH HHS/United States
GR  - U01 AI034993/AI/NIAID NIH HHS/United States
GR  - P30 AI050410/AI/NIAID NIH HHS/United States
GR  - K01 HL137557/HL/NHLBI NIH HHS/United States
GR  - U01 HD032632/HD/NICHD NIH HHS/United States
GR  - U01 AI042590/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cardiovasc Res
JT  - Cardiovascular research
JID - 0077427
RN  - 0 (Biomarkers)
RN  - 0 (CXCR4 protein, human)
RN  - 0 (Receptors, CXCR4)
SB  - IM
CIN - Cardiovasc Res. 2019 May 1;115(6):989-991. PMID: 30830148
MH  - Adult
MH  - Antiretroviral Therapy, Highly Active
MH  - Asymptomatic Diseases
MH  - Biomarkers/analysis
MH  - Carotid Artery Diseases/diagnosis/*immunology
MH  - Cross-Sectional Studies
MH  - Down-Regulation
MH  - Female
MH  - HIV Infections/diagnosis/drug therapy/*immunology
MH  - Humans
MH  - Middle Aged
MH  - Monocytes/classification/drug effects/*immunology
MH  - Phenotype
MH  - Plaque, Atherosclerotic
MH  - Receptors, CXCR4/*analysis
MH  - Sex Factors
PMC - PMC6735712
OTO - NOTNLM
OT  - Atherosclerosis
OT  - CXCR4
OT  - Cardiovascular risk assessment
OT  - HIV
OT  - Non-classical monocytes
OT  - Women
EDAT- 2018/12/07 06:00
MHDA- 2020/05/19 06:00
PMCR- 2020/05/01
CRDT- 2018/12/07 06:00
PHST- 2018/05/16 00:00 [received]
PHST- 2018/11/07 00:00 [revised]
PHST- 2018/12/04 00:00 [accepted]
PHST- 2018/12/07 06:00 [pubmed]
PHST- 2020/05/19 06:00 [medline]
PHST- 2018/12/07 06:00 [entrez]
PHST- 2020/05/01 00:00 [pmc-release]
AID - 5231867 [pii]
AID - cvy292 [pii]
AID - 10.1093/cvr/cvy292 [doi]
PST - ppublish
SO  - Cardiovasc Res. 2019 May 1;115(6):1029-1040. doi: 10.1093/cvr/cvy292.