PMID- 30522172
OWN - NLM
STAT- MEDLINE
DCOM- 20190226
LR  - 20190226
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 47
IP  - 12
DP  - 2018 Dec 8
TI  - [Diagnostic and therapeutic values of interphase fluorescence in situ 
      hybridization in B-cell lymphomas: a clinicopathologic analysis of 604 cases].
PG  - 920-925
LID - 10.3760/cma.j.issn.0529-5807.2018.12.005 [doi]
AB  - Objective: To investigate the feasibility and value of interphase fluorescence in 
      situ hybridization (FISH) in the pathological diagnosis, differential diagnosis 
      and therapeutic assessment of B-cell lymphomas. Methods: The cohort included 604 
      cases of B-cell lymphoma which were collected at West China Hospital from May 
      2010 to December 2016.And all were subjected to interphase FISH using 11 break 
      apart or fusion probes (MYC, bcl-2, bcl-6, IRF4, MYC/IgH, bcl-2/IgH, CCND1/IgH, 
      IgH, API2/MALT1, p53/ATM, and D13S319/CEP12). Results: The median age of the 604 
      B-cell lymphoma patients was 47.7 (aged 2-90) years including 372 men and 232 
      women. All the cases was divided into 463 large B cell lymphomas(LBL) and 141 
      small B cell lymphomas, and the total interphase FISH positive rate was 59.8% 
      (361/604). Among the 463 LBL, 12.5% (58/463), 9.5% (44/463) and 2.2% (10/463) of 
      cases showed MYC, bcl-6 and bcl-2 gene rearrangements respectively; and 363 
      diffuse large B cell lymphoma (DLBCLs) were reclassified as germinal center 
      B-cell (GCB) subtype (38.6%, 140/363) and non-GCB subtype (61.4%, 223/363) by 
      Hans algorithm. The rearrangement rates in GCB and non-GCB DLBCL were 
      45.7%(64/140)and 21.5%(48/223; P=0.001), respectively. Compared to the non-GCB 
      DLBCL, GCB DLBCL showed higher MYC and bcl-2 gene rearrangements (P=0.001). 
      Eleven (2.4%, 11/463) cases had MYC and bcl-6 or bcl-2 gene rearrangement 
      (double-hit lymphoma); one (0.2%, 1/463) case had MYC, bcl-6 and bcl-2 gene 
      rearrangements (triple-hit lymphoma); two (0.4%, 2/463) cases had bcl-2 and bcl-6 
      gene rearrangements. MYC translocation and MYC/IgH fusion were detected in 
      94.2%(81/86) and 83.7%(72/86) cases of Burkitt lymphomas. IRF4 rearrangement was 
      detected in two cases of IRF4+ LBCL. Genetic abnormalities were detected in 9/19, 
      100%(29/29), 30.8%(12/39) and 68.5%(37/54) cases of follicular lymphoma, mantle 
      cell lymphoma, MALT lymphoma and chronic lymphocytic leukemia, respectively. 
      Conclusions: Interphase FISH can rapidly and accurately detect the genetic 
      changes in B-cell lymphomas. Different genetic changes are specifically valuable 
      to the diagnosis, differential diagnosis, prognosis evaluation and treatment 
      guidance of various B-cell lymphomas.
FAU - Chen, M
AU  - Chen M
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Yang, J L
AU  - Yang JL
FAU - Zhao, S
AU  - Zhao S
FAU - Liu, W P
AU  - Liu WP
FAU - Li, G D
AU  - Li GD
FAU - Ye, Y X
AU  - Ye YX
FAU - Yan, J Q
AU  - Yan JQ
FAU - Zhang, W Y
AU  - Zhang WY
LA  - chi
GR  - 2017JY0266/Application Basic Research Project Founded by Science & Technology 
      Department of Sichuan Province/
GR  - 16ZD009, 16PJ337/Key Program Founded by Health and Family Planning Commission of 
      Sichuan Province/
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - China
MH  - Feasibility Studies
MH  - Female
MH  - Gene Rearrangement, B-Lymphocyte
MH  - Genes, bcl-2/*genetics
MH  - Genes, myc/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphoma, B-Cell/classification/*genetics/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-bcl-6/genetics
MH  - Translocation, Genetic
MH  - Young Adult
OTO - NOTNLM
OT  - Gene fusion
OT  - In situ hybridization, fluorescence
OT  - Lymphoma, B-cell
OT  - Translocation, genetic
EDAT- 2018/12/14 06:00
MHDA- 2019/02/27 06:00
CRDT- 2018/12/08 06:00
PHST- 2018/12/08 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/02/27 06:00 [medline]
AID - 10.3760/cma.j.issn.0529-5807.2018.12.005 [doi]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2018 Dec 8;47(12):920-925. doi: 
      10.3760/cma.j.issn.0529-5807.2018.12.005.