PMID- 30523440
OWN - NLM
STAT- MEDLINE
DCOM- 20200207
LR  - 20200207
IS  - 1432-0711 (Electronic)
IS  - 0932-0067 (Linking)
VI  - 299
IP  - 2
DP  - 2019 Feb
TI  - Arrangement of myofibroblastic and smooth muscle-like cells in superficial 
      peritoneal endometriosis and a possible role of transforming growth factor beta 1 
      (TGFbeta1) in myofibroblastic metaplasia.
PG  - 489-499
LID - 10.1007/s00404-018-4995-y [doi]
AB  - PURPOSE: Superficial peritoneal endometriotic (pEM) lesions are composed of 
      endometrial glands and stroma, in addition to a third component-myofibroblasts 
      and smooth muscles (SM)-like cells. The latter develops secondary to a 
      metaplasia. In this study, we characterised the third component cells in pEM 
      according to differentiation markers in different micro-compartments. 
      Furthermore, a possible effect of TGFbeta1 on myofibroblastic metaplasia in 
      endometriotic epithelial cells was studied. METHODS: Seventy-six premenopausal 
      patients were included. Peritoneal biopsies were excised from EM patients 
      (n = 23), unaffected peritoneum (peritoneum from EM patients but without EM 
      components, n = 5/23) and non-EM patients (n = 10). All peritoneal biopsies were 
      immunolabeled for ASMA, calponin, collagen I, desmin, TGFss receptor 1 (R1), R2 
      and R3 in addition to ultrastructure examination by transmission electron 
      microscopy (TEM) (n = 1). TGFss1 level was measured in peritoneal fluid (PF) (EM, 
      n = 19 and non-EM, n = 13) collected during laparoscopy. Furthermore, TGFss1 
      effect on myofibroblastic metaplasia was studied in vitro. RESULTS: At the centre 
      of pEM lesions, calponin immunolabeling outweighs the collagen I while in the 
      periphery the reverse occurs. SM-like cells expressing desmin predominate at the 
      periphery, while ASMA immunolabeling was detectable in all micro-compartments. 
      Both indicate an abundance of myofibroblasts at the centre of pEM lesions and 
      SM-like cells in the periphery. Although activated TGFss1 in PF did not differ 
      between EM and non-EM, it inhibited the cell proliferation of the endometriotic 
      epithelial cells and induced an upregulation in ASMA and collagen IA2 expression 
      as well. CONCLUSION: The abundance of the myofibroblasts and SM-like cells points 
      to a myofibroblastic metaplasia in pEM. Both cells are differentially arranged in 
      the different micro-compartments of pEM lesions, with increasing cell maturity 
      towards the periphery of the lesion. Furthermore, TGFss1 may play a role in the 
      myofibroblastic metaplasia of the endometriotic epithelial cells. These findings 
      provide a better insight in the micro-milieu in EM lesions, where most of the 
      disease dynamics occur.
FAU - Ibrahim, Mohamed Gamal
AU  - Ibrahim MG
AUID- ORCID: 0000-0001-6648-9281
AD  - Clinic for Gynaecology, Charite University of Medicine, Hindenburgdamm 30, 12203, 
      Berlin, Germany.
AD  - Department of Gynecology and Obstetrics, UKM Fertility Center, University 
      Hospital of Muenster, Domagkstr. 11, 48149, Munster, Germany.
FAU - Sillem, Martin
AU  - Sillem M
AD  - Universitats-Frauenklinik Homburg/Saar und Praxisklinik am Rosengarten, 
      Augustaanlage 7-11, 68165, Mannheim, Germany.
FAU - Plendl, Johanna
AU  - Plendl J
AD  - Department of Veterinary Medicine, Institute of Veterinary Anatomy, Free 
      University of Berlin, Berlin, Germany.
FAU - Taube, Eliane T
AU  - Taube ET
AD  - Institute for Pathology, Charite University of Medicine, Chariteplatz 1, 10117, 
      Berlin, Germany.
FAU - Schuring, Andreas
AU  - Schuring A
AD  - Department of Gynecology and Obstetrics, UKM Fertility Center, University 
      Hospital of Muenster, Domagkstr. 11, 48149, Munster, Germany.
FAU - Gotte, Martin
AU  - Gotte M
AD  - Department of Gynecology and Obstetrics, UKM Fertility Center, University 
      Hospital of Muenster, Domagkstr. 11, 48149, Munster, Germany.
FAU - Chiantera, Vito
AU  - Chiantera V
AD  - Clinic for Gynaecology, Charite University of Medicine, Hindenburgdamm 30, 12203, 
      Berlin, Germany.
FAU - Sehouli, Jalid
AU  - Sehouli J
AD  - Clinic for Gynaecology, Charite University of Medicine, Hindenburgdamm 30, 12203, 
      Berlin, Germany.
FAU - Mechsner, Sylvia
AU  - Mechsner S
AD  - Clinic for Gynaecology, Charite University of Medicine, Hindenburgdamm 30, 12203, 
      Berlin, Germany. sylvia.mechsner@charite.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181207
PL  - Germany
TA  - Arch Gynecol Obstet
JT  - Archives of gynecology and obstetrics
JID - 8710213
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Adult
MH  - Cell Differentiation
MH  - Endometriosis/*physiopathology
MH  - Female
MH  - Humans
MH  - Metaplasia
MH  - Muscle, Smooth/*metabolism
MH  - Myofibroblasts/*metabolism
MH  - Peritoneal Diseases/*physiopathology
MH  - Peritoneum/*physiopathology
MH  - Transforming Growth Factor beta1/*metabolism
OTO - NOTNLM
OT  - Myofibroblastic metaplasia
OT  - Peritoneal endometriosis
OT  - Smooth muscle-like cells
OT  - TGFbeta1
EDAT- 2018/12/14 06:00
MHDA- 2020/02/08 06:00
CRDT- 2018/12/08 06:00
PHST- 2018/05/14 00:00 [received]
PHST- 2018/11/24 00:00 [accepted]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2020/02/08 06:00 [medline]
PHST- 2018/12/08 06:00 [entrez]
AID - 10.1007/s00404-018-4995-y [pii]
AID - 10.1007/s00404-018-4995-y [doi]
PST - ppublish
SO  - Arch Gynecol Obstet. 2019 Feb;299(2):489-499. doi: 10.1007/s00404-018-4995-y. 
      Epub 2018 Dec 7.