PMID- 30524287
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240404
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 9
DP  - 2018
TI  - Unraveling the Novel Protective Effect of Patchouli Alcohol Against Helicobacter 
      pylori-Induced Gastritis: Insights Into the Molecular Mechanism in vitro and in 
      vivo.
PG  - 1347
LID - 10.3389/fphar.2018.01347 [doi]
LID - 1347
AB  - Patchouli alcohol (PA), a natural tricyclic sesquiterpene extracted from 
      Pogostemon cablin (Blanco) Benth. (Labiatae), has been found to exhibit 
      anti-Helicobacter pylori and anti-inflammatory properties. In this study, we 
      investigated the protective effect of PA against H. pylori-induced gastritis in 
      vitro and in vivo, and determined the underlying mechanism. In the in vivo 
      experiment, a C57BL/6 mouse model of gastritis was established using H. pylori 
      SS1, and treatments with standard triple therapy or 5, 10, and 20 mg/kg PA were 
      performed for 2 weeks. Results indicated that PA effectively attenuated oxidative 
      stress by decreasing contents of intracellular reactive oxygen species (ROS) and 
      malonyldialdehyde (MDA), and increasing levels of non-protein sulfhydryl (NP-SH), 
      catalase and glutathione (GSH)/glutathione disulphide (GSSG). Additionally, 
      treatment with PA significantly attenuated the secretions of interleukin 1 beta 
      (IL-1beta), keratinocyte chemoattractant and interleukin 6 (IL-6). PA (20 mg/kg) 
      significantly protected the gastric mucosa from H. pylori-induced damage. In the 
      in vitro experiment, GES-1 cells were cocultured with H. pylori NCTC11637 at MOI 
      = 100:1 and treated with different doses of PA (5, 10, and 20 mug/ml). Results 
      indicated that PA not only significantly increased the cell viability and 
      decreased cellular lactate dehydrogenase (LDH) leakage, but also markedly 
      elevated the mitochondrial membrane potential and remarkably attenuated GES-1 
      cellular apoptosis, thereby protecting gastric epithelial cells against injuries 
      caused by H. pylori. PA also inhibited the secretions of pro-inflammatory 
      factors, such as monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor-alpha 
      (TNF-alpha) and IL-6. Furthermore, after PA treatment, the combination of NACHT, LRR, 
      and PYD domains-containing protein 3 (NLRP3) and cysteine-aspartic proteases 1 
      (CASPASE-1), the expression levels of NLRP3 inflammasome-related proteins, such 
      as thioredoxin-interacting protein (TXNIP), pro-CASPASE-1, cle-CASPASE-1, and 
      NLRP3 and genes (NLRP3 and CASPASE1) were significantly decreased as compared to 
      the model group. In conclusion, treatment with PA for 2 weeks exhibited highly 
      efficient protective effect against H. pylori-induced gastritis and related 
      damages. The underlying mechanism might involve antioxidant activity, inhibition 
      of pro-inflammatory factor and regulation of NLRP3 inflammasome function. PA 
      exerted anti-H. pylori and anti-gastritis effects and thus had the potential to 
      be a promising candidate for treatment of H. pylori-related diseases.
FAU - Lian, Da-Wei
AU  - Lian DW
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Xu, Yi-Fei
AU  - Xu YF
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Ren, Wen-Kang
AU  - Ren WK
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Fu, Li-Jun
AU  - Fu LJ
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Chen, Fang-Jun
AU  - Chen FJ
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Tang, Li-Yao
AU  - Tang LY
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Zhuang, Hui-Ling
AU  - Zhuang HL
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Cao, Hong-Ying
AU  - Cao HY
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Huang, Ping
AU  - Huang P
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
AD  - Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of 
      Mathematical Engineering for Chinese Medicine, Guangzhou University of Chinese 
      Medicine, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20181122
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC6262355
OTO - NOTNLM
OT  - Helicobacter pylori
OT  - gastric epithelial cell
OT  - gastritis
OT  - inflammasome
OT  - oxidative injury
OT  - patchouli alcohol
EDAT- 2018/12/14 06:00
MHDA- 2018/12/14 06:01
PMCR- 2018/11/22
CRDT- 2018/12/08 06:00
PHST- 2018/05/13 00:00 [received]
PHST- 2018/10/31 00:00 [accepted]
PHST- 2018/12/08 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2018/12/14 06:01 [medline]
PHST- 2018/11/22 00:00 [pmc-release]
AID - 10.3389/fphar.2018.01347 [doi]
PST - epublish
SO  - Front Pharmacol. 2018 Nov 22;9:1347. doi: 10.3389/fphar.2018.01347. eCollection 
      2018.