PMID- 30524427
OWN - NLM
STAT- MEDLINE
DCOM- 20191002
LR  - 20221207
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - Suppression of Intestinal Epithelial Cell Chemokine Production by Lactobacillus 
      rhamnosus R0011 and Lactobacillus helveticus R0389 Is Mediated by Secreted 
      Bioactive Molecules.
PG  - 2639
LID - 10.3389/fimmu.2018.02639 [doi]
LID - 2639
AB  - Host intestinal epithelial cells (IEC) present at the gastrointestinal interface 
      are exposed to pathogenic and non-pathogenic bacteria and their products. Certain 
      probiotic lactic acid bacteria (LAB) have been associated with a range of 
      host-immune modulatory activities including down-regulation of pro-inflammatory 
      gene expression and cytokine production by IEC, with growing evidence suggesting 
      that these bacteria secrete bioactive molecules with immunomodulatory activity. 
      The aim of this study was to determine whether two lactobacilli with 
      immunomodulatory activity [Lactobacillus rhamnosus R0011 (Lr) and Lactobacillus 
      helveticus R0389 (Lh)], produce soluble mediators able to influence IEC responses 
      to Pattern Recognition Receptor (PRR) ligands and pro-inflammatory cytokines 
      [Tumor Necrosis Factor alpha (TNFalpha), Interleukin-1beta (IL-1beta)], signals inducing IEC 
      chemokine production during infection. To this end, the effects of cell-free 
      supernatants (CFS) from Lr and Lh on IEC production of the pro-inflammatory 
      chemokines interleukin (IL)-8 and cytokine-induced neutrophil chemoattractant 1 
      (CINC-1) induced by a range of host- or pathogen-derived pro-inflammatory stimuli 
      were determined, and the impact on human HT-29 IEC and a primary IEC line (rat 
      IEC-6) was compared. The Lr-CFS and Lh-CFS did not significantly modulate basal 
      IL-8 production from HT-29 IECs or CINC-1 production from IEC-6 cells. However, 
      both Lr-CFS and Lh-CFS significantly down-regulated IL-8 production from HT-29 
      IECs challenged with varied PRR ligands. Lr-CFS and Lh-CFS had differential 
      effects on PRR-induced CINC-1 production by rat IEC-6 IECs, with no significant 
      down-regulation of CINC-1 observed from IEC-6 IECs cultured with Lh-CFS. Further 
      analysis of the Lr-CFS revealed down-regulation of IL-8 production induced by the 
      pro-inflammatory cytokines IL-1beta and TNFalpha Preliminary characterization of the 
      bioactive constituent(s) of the Lr-CFS indicates that it is resistant to 
      treatment with DNase, RNase, and an acidic protease, but is sensitive to 
      alterations in pH. Taken together, these results indicate that these lactobacilli 
      secrete bioactive molecules of low molecular weight that may modulate host innate 
      immune activity through interactions with IEC.
FAU - Jeffrey, Michael P
AU  - Jeffrey MP
AD  - Applied Bioscience Graduate Program, University of Ontario Institute of 
      Technology, Oshawa, ON, Canada.
FAU - Strap, Janice L
AU  - Strap JL
AD  - Applied Bioscience Graduate Program, University of Ontario Institute of 
      Technology, Oshawa, ON, Canada.
AD  - Faculty of Science, University of Ontario Institute of Technology, Oshawa, ON, 
      Canada.
FAU - Jones Taggart, Holly
AU  - Jones Taggart H
AD  - Applied Bioscience Graduate Program, University of Ontario Institute of 
      Technology, Oshawa, ON, Canada.
AD  - Faculty of Health Sciences, University of Ontario Institute of Technology, 
      Oshawa, ON, Canada.
FAU - Green-Johnson, Julia M
AU  - Green-Johnson JM
AD  - Applied Bioscience Graduate Program, University of Ontario Institute of 
      Technology, Oshawa, ON, Canada.
AD  - Faculty of Science, University of Ontario Institute of Technology, Oshawa, ON, 
      Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181114
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Chemokines)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Chemokines/*immunology
MH  - Epithelial Cells/*immunology/*microbiology
MH  - Humans
MH  - Intestinal Mucosa/*immunology/*microbiology
MH  - Lactobacillus helveticus/*immunology
MH  - Lacticaseibacillus rhamnosus/*immunology
MH  - Rats
PMC - PMC6262363
OTO - NOTNLM
OT  - CINC-1
OT  - IL-8
OT  - Lactobacillus helveticus R0389
OT  - Lactobacillus rhamnosus R0011
OT  - PRR
OT  - chemokine
OT  - secretome
EDAT- 2018/12/14 06:00
MHDA- 2019/10/03 06:00
PMCR- 2018/01/01
CRDT- 2018/12/08 06:00
PHST- 2018/07/27 00:00 [received]
PHST- 2018/10/26 00:00 [accepted]
PHST- 2018/12/08 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/10/03 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.02639 [doi]
PST - epublish
SO  - Front Immunol. 2018 Nov 14;9:2639. doi: 10.3389/fimmu.2018.02639. eCollection 
      2018.