PMID- 30529208
OWN - NLM
STAT- MEDLINE
DCOM- 20190531
LR  - 20190531
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 126
DP  - 2019 Apr 1
TI  - Identification and characterization of a novel thermostable xylanase from camel 
      rumen metagenome.
PG  - 1295-1302
LID - S0141-8130(18)33763-2 [pii]
LID - 10.1016/j.ijbiomac.2018.12.041 [doi]
AB  - Metagenomics has emerged to isolate novel enzymes from the uncultured microbiota 
      in the environment. In this study, the metagenomic data obtained from camel rumen 
      was considered as the potential source of microbial xylanase enzymes with proper 
      activity in extreme conditions. The metagenomic data were assembled and contigs 
      were used for in-silico identification of candidate thermostable enzyme. A novel 
      thermostable xylanase enzyme, named PersiXyn1, with 1146 bp full-length gene 
      which encodes a 381 amino acid protein was identified. Using the DNA template 
      extracted from camel rumen metagenomic samples, the candidate enzyme genes were 
      cloned and expressed in proper E. coli strains. The phylogenetic analysis showed 
      the evolutionary position of PersiXyn1 among the known thermostable xylanases. 
      The results of the CD analysis and determining the secondary structure of the 
      enzyme, confirmed the presence of a high percentage of beta-sheets as an important 
      characteristic of thermophilic xylanases. The PersiXyn1 was active at a broad 
      range of pH (6-11) and temperature (25-90  degrees C). The optimum pH and temperature 
      were 8 and 40  degrees C respectively, and the enzyme maintained 80% of its maximum 
      activity in the pH 8 and temperature 40  degrees C for 1 h. The Scanning electron 
      microscope (SEM) micrograph of enzyme treated pulp clearly showed that the 
      effective use of enzymes in fiber separation may reduce the cost of carton paper 
      production. The novelty of this enzyme lies in the fact that it is highly active 
      and stable in a broad range of pH and temperature. This study highlights the 
      potential importance of camel microbiome for discovering novel thermostable 
      enzymes with applications in agriculture and industries.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Ariaeenejad, Shohreh
AU  - Ariaeenejad S
AD  - Department of Systems Biology, Agricultural Biotechnology Research Institute of 
      Iran (ABRII), Agricultural Research Education and Extension Organization (AREO), 
      Karaj, Iran. Electronic address: sh.ariaee@abrii.ac.ir.
FAU - Hosseini, Elnaz
AU  - Hosseini E
AD  - Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, 
      Iran.
FAU - Maleki, Morteza
AU  - Maleki M
AD  - Department of Systems Biology, Agricultural Biotechnology Research Institute of 
      Iran (ABRII), Agricultural Research Education and Extension Organization (AREO), 
      Karaj, Iran.
FAU - Kavousi, Kaveh
AU  - Kavousi K
AD  - Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, 
      Iran.
FAU - Moosavi-Movahedi, Ali A
AU  - Moosavi-Movahedi AA
AD  - Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, 
      Iran.
FAU - Salekdeh, Ghasem Hosseini
AU  - Salekdeh GH
AD  - Department of Systems Biology, Agricultural Biotechnology Research Institute of 
      Iran (ABRII), Agricultural Research Education and Extension Organization (AREO), 
      Karaj, Iran. Electronic address: h_salekdeh@abrii.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20181204
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - EC 3.2.1.8 (Endo-1,4-beta Xylanases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Camelus/*microbiology
MH  - Catalytic Domain
MH  - Circular Dichroism
MH  - Endo-1,4-beta Xylanases/*metabolism
MH  - Enzyme Stability
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - *Metagenome
MH  - Paper
MH  - Phylogeny
MH  - Protein Unfolding
MH  - Rumen/*enzymology
MH  - *Temperature
OTO - NOTNLM
OT  - Circular dichroism
OT  - Metagenome, protein expression
OT  - Novel xylanase
OT  - Thermal unfolding
EDAT- 2018/12/12 06:00
MHDA- 2019/06/01 06:00
CRDT- 2018/12/12 06:00
PHST- 2018/07/27 00:00 [received]
PHST- 2018/11/19 00:00 [revised]
PHST- 2018/12/02 00:00 [accepted]
PHST- 2018/12/12 06:00 [pubmed]
PHST- 2019/06/01 06:00 [medline]
PHST- 2018/12/12 06:00 [entrez]
AID - S0141-8130(18)33763-2 [pii]
AID - 10.1016/j.ijbiomac.2018.12.041 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 Apr 1;126:1295-1302. doi: 
      10.1016/j.ijbiomac.2018.12.041. Epub 2018 Dec 4.