PMID- 30532196
OWN - NLM
STAT- MEDLINE
DCOM- 20190506
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 12
DP  - 2018
TI  - Zoonotic multidrug-resistant microorganisms among small companion animals in 
      Germany.
PG  - e0208364
LID - 10.1371/journal.pone.0208364 [doi]
LID - e0208364
AB  - Antimicrobial multidrug-resistant microorganisms (MDRO) can be transmitted 
      between companion animals and their human owners. Aim of this study was to 
      determine the prevalence of extended spectrum beta-lactamase-producing 
      Enterobacteriaceae (ESBL-E) and Staphylococcus aureus including 
      methicillin-resistant S. aureus (MRSA) in different companion animal species. 
      Dogs (n = 192), cats (n = 74), and rabbits (n = 17), treated in a veterinary 
      practice and hospital or living in an animal shelter and private households, were 
      sampled. All facilities were located in a region characterized by a high density 
      of pig production. Nasal, buccal and perianal swabs were enriched and cultured on 
      solid chromogenic selective media. A subgroup of 20 animals (13 dogs, 3 cats, 4 
      rabbits) was analyzed for the presence of staphylococci other than S. aureus. 
      Amongst all animals (n = 283), twenty dogs (10.4%) and six cats (8.1%) carried S. 
      aureus. MRSA was found in five dogs (2.6%) and two cats (2.7%). Isolates were of 
      spa types t011, t034, t108 (all mecA-positive, ST398), and t843 (mecC-positive, 
      ST130), typical for livestock-associated (LA)-MRSA. Except for one dog, 
      MRSA-positive animals did not have direct contact to husbandry. ESBL-Escherichia 
      coli (blaCTX-M/blaTEM/blaSHV genes) were present in seven dogs (3.6%), one cat 
      (1.4%) possessed a cefotaxime-resistant Citrobacter freundii isolate 
      (blaTEM/blaCMY-2 genes). MDRO carriage was associated with animals from 
      veterinary medical settings (p<0.05). One dog and one rabbit carried 
      methicillin-resistant coagulase-negative staphylococci. The exclusive occurrence 
      of MRSA lineages typically described for livestock stresses the impact of MDRO 
      strain dissemination across species barriers in regional settings. Presence of 
      ESBL-E and LA-MRSA among pets and probable dissemination in clinical settings 
      support the necessity of a "One Health" approach to address the potential threats 
      due to MDRO-carrying companion animals.
FAU - Kaspar, Ursula
AU  - Kaspar U
AD  - Institute of Medical Microbiology, University Hospital Munster, Munster, Germany.
FAU - von Lutzau, Alexa
AU  - von Lutzau A
AD  - Institute of Medical Microbiology, University Hospital Munster, Munster, Germany.
FAU - Schlattmann, Andreas
AU  - Schlattmann A
AD  - Institute of Medical Microbiology, University Hospital Munster, Munster, Germany.
FAU - Roesler, Uwe
AU  - Roesler U
AD  - Institute for Animal Hygiene and Environmental Health, FU Berlin, Department of 
      Veterinary Medicine, Berlin, Germany.
FAU - Kock, Robin
AU  - Kock R
AD  - Institute of Medical Microbiology, University Hospital Munster, Munster, Germany.
FAU - Becker, Karsten
AU  - Becker K
AUID- ORCID: 0000-0002-6391-1341
AD  - Institute of Medical Microbiology, University Hospital Munster, Munster, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181207
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (beta-Lactams)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology/*therapeutic use
MH  - Cat Diseases/drug therapy/microbiology
MH  - Cats
MH  - Dog Diseases/drug therapy/microbiology
MH  - Dogs
MH  - Drug Resistance, Multiple, Bacterial
MH  - Enterobacteriaceae/drug effects
MH  - Germany
MH  - Methicillin-Resistant Staphylococcus aureus/drug effects
MH  - Microbial Sensitivity Tests
MH  - Rabbits
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Staphylococcus aureus/drug effects
MH  - beta-Lactams/pharmacology/therapeutic use
PMC - PMC6285998
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/12/12 06:00
MHDA- 2019/05/07 06:00
PMCR- 2018/12/07
CRDT- 2018/12/12 06:00
PHST- 2018/10/01 00:00 [received]
PHST- 2018/11/15 00:00 [accepted]
PHST- 2018/12/12 06:00 [entrez]
PHST- 2018/12/12 06:00 [pubmed]
PHST- 2019/05/07 06:00 [medline]
PHST- 2018/12/07 00:00 [pmc-release]
AID - PONE-D-18-27339 [pii]
AID - 10.1371/journal.pone.0208364 [doi]
PST - epublish
SO  - PLoS One. 2018 Dec 7;13(12):e0208364. doi: 10.1371/journal.pone.0208364. 
      eCollection 2018.