PMID- 30534157
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2018
DP  - 2018
TI  - Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs.
PG  - 5912194
LID - 10.1155/2018/5912194 [doi]
LID - 5912194
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) are superior to other 
      sources of mesenchymal stem/stromal cells (MSCs), and they are used as a novel 
      tool for cell-based cancer therapy. However, the mechanism underlying 
      hUCMSC-induced cancer cell death is not clear. In the present study, we aimed to 
      evaluate the effect of secreted factors of hUCMSCs on the breast cancer cell line 
      MCF7 by exposing them to the conditioned medium (CM) of hUCMSCs. We evaluated the 
      morphological changes, cell viability, cell cycle, apoptosis, DNA fragmentation, 
      and interleukin-1beta (IL-1beta) secretion of CM-exposed MCF7 cells. The results showed 
      that the secreted factors of hUCMSCs could cause MCF7 cell death by inducing 
      pyroptosis. We also sequenced the total RNA, and the differentially expressed 
      genes (DEGs) were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) 
      pathway analysis. A total of 2597 (1822 upregulated and 775 downregulated) genes 
      were identified and 14 pathways were significantly enriched. The results showed 
      that the expression of the pyroptosis-related genes NLRP1 and CASP4 and the 
      inflammation-related pathways changed significantly in MCF7 cells exposed to the 
      CM. To the best of our knowledge, this study is the first to report that the 
      secreted factors of hUCMSCs can cause MCF7 cell pyroptosis. Furthermore, it is 
      the first to examine the global gene expression in MCF7 cells exposed to CM. 
      These results will provide valuable information for further studies on the 
      mechanism of MCF7 cell pyroptosis induced by the secreted factors of hUCMSCs. It 
      will also help understand the effect of hUCMSCs on cell-based breast cancer 
      therapy.
FAU - Jiao, Yang
AU  - Jiao Y
AD  - School of Medicine, Yunnan University, Kunming, 650091 Yunnan, China.
AD  - Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of 
      Molecular and Clinical medicine, Kunming Medical University, Kunming, 650500 
      Yunnan, China.
FAU - Zhao, Hongbo
AU  - Zhao H
AD  - Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of 
      Molecular and Clinical medicine, Kunming Medical University, Kunming, 650500 
      Yunnan, China.
FAU - Chen, Guodong
AU  - Chen G
AD  - School of Medicine, Yunnan University, Kunming, 650091 Yunnan, China.
FAU - Sang, Xiongbo
AU  - Sang X
AD  - School of Medicine, Yunnan University, Kunming, 650091 Yunnan, China.
FAU - Yang, Luhan
AU  - Yang L
AD  - School of Medicine, Yunnan University, Kunming, 650091 Yunnan, China.
FAU - Hou, Zongliu
AU  - Hou Z
AUID- ORCID: 0000-0003-3536-7550
AD  - Yan'an Hospital of Kunming City, Kunming, 650051 Yunnan, China.
FAU - Si, Wei
AU  - Si W
AUID- ORCID: 0000-0003-0861-0682
AD  - Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate 
      Translational Medicine, Kunming University of Science and Technology, Kunming, 
      650500 Yunnan, China.
FAU - Zheng, Bingrong
AU  - Zheng B
AUID- ORCID: 0000-0001-9620-286X
AD  - School of Medicine, Yunnan University, Kunming, 650091 Yunnan, China.
LA  - eng
PT  - Journal Article
DEP - 20181111
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC6252231
EDAT- 2018/12/12 06:00
MHDA- 2018/12/12 06:01
PMCR- 2018/11/11
CRDT- 2018/12/12 06:00
PHST- 2018/06/14 00:00 [received]
PHST- 2018/08/22 00:00 [revised]
PHST- 2018/09/16 00:00 [accepted]
PHST- 2018/12/12 06:00 [entrez]
PHST- 2018/12/12 06:00 [pubmed]
PHST- 2018/12/12 06:01 [medline]
PHST- 2018/11/11 00:00 [pmc-release]
AID - 10.1155/2018/5912194 [doi]
PST - epublish
SO  - Stem Cells Int. 2018 Nov 11;2018:5912194. doi: 10.1155/2018/5912194. eCollection 
      2018.