PMID- 30535928
OWN - NLM
STAT- MEDLINE
DCOM- 20190708
LR  - 20200225
IS  - 1436-2236 (Electronic)
IS  - 1436-2228 (Linking)
VI  - 21
IP  - 2
DP  - 2019 Apr
TI  - Biocalcification by Piezotolerant Bacillus sp. NIOTVJ5 Isolated from Deep Sea 
      Sediment and its Influence on the Strength of Concrete Specimens.
PG  - 161-170
LID - 10.1007/s10126-018-9867-8 [doi]
AB  - Biocalcification or microbially induced carbonate precipitation (MICP) is gaining 
      attention from the research fraternity, primarily ascribed to their eco-friendly 
      applications. Bacterial strains have been isolated from various sources and their 
      ability to precipitate carbonate has been studied extensively. In spite of the 
      fact that the deep-sea environment is a potential source for bioprospecting, 
      meager reports exist on the isolation of biocalcifying bacterial strains from 
      deep-sea. In this study, a deep-sea sediment sample obtained from off-Barren 
      Island coast in the Andaman Sea was investigated for biocalcifying strains. Based 
      on the urease activity and the ability to produce calcite crystals, the strain 
      NIOTVJ5 was chosen for further investigations. The strain showed a similarity to 
      Bacillus thuringiensis through 16S rRNA sequencing and was shown to possess 
      positive urease, protease, amylase, catalase, and oxidase activities. The isolate 
      was found to be piezotolerant as it was able to survive at 100 bar pressure with 
      significant changes in the spore morphology. The strain was able to produce 
      strong monoxenic biofilms as well. Maximum urease activity was 554.03 U/mL and it 
      precipitated 1.80 g/L of carbonate crystals. Scanning electron microscopy coupled 
      with energy dispersive X-ray spectroscopy confirmed the presence of calcium 
      carbonate. The carbonate polymorph was identified as calcite using X-ray powder 
      diffraction. The impact of biocalcification by NIOTVJ5 on concrete specimens 
      indicated an increase of 30.91% in their compressive strength. This is the first 
      report of a biocalcifying strain from a deep-sea sediment around the Indian 
      subcontinent region. This study indicates the potential of the strain NIOTVJ5, 
      which can be employed for various biotechnological applications.
FAU - Rangamaran, Vijaya Raghavan
AU  - Rangamaran VR
AD  - Department of Biotechnology, School of Biosciences and Technology, VIT 
      University, Vellore, Tamil Nadu, India.
FAU - Shanmugam, Venkat Kumar
AU  - Shanmugam VK
AD  - Department of Biotechnology, School of Biosciences and Technology, VIT 
      University, Vellore, Tamil Nadu, India. venkatkumars@vit.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20181210
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacillus/enzymology/isolation & purification/*metabolism
MH  - Biofilms
MH  - Calcium Carbonate/chemistry/*metabolism
MH  - Compressive Strength
MH  - Construction Materials
MH  - Indian Ocean
MH  - Pressure
MH  - RNA, Ribosomal, 16S
MH  - Urease/metabolism
OTO - NOTNLM
OT  - Bacillus sp.
OT  - Biofilm
OT  - Calcite
OT  - Deep-sea sediment
OT  - MICP
OT  - Urease
EDAT- 2018/12/12 06:00
MHDA- 2019/07/10 06:00
CRDT- 2018/12/12 06:00
PHST- 2018/08/23 00:00 [received]
PHST- 2018/11/29 00:00 [accepted]
PHST- 2018/12/12 06:00 [pubmed]
PHST- 2019/07/10 06:00 [medline]
PHST- 2018/12/12 06:00 [entrez]
AID - 10.1007/s10126-018-9867-8 [pii]
AID - 10.1007/s10126-018-9867-8 [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2019 Apr;21(2):161-170. doi: 10.1007/s10126-018-9867-8. Epub 
      2018 Dec 10.