PMID- 30536089
OWN - NLM
STAT- MEDLINE
DCOM- 20190611
LR  - 20190613
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1905
DP  - 2019
TI  - Genetic Lineage Tracing of Biliary Epithelial Cells.
PG  - 45-57
LID - 10.1007/978-1-4939-8961-4_5 [doi]
AB  - Lineage tracing of liver cells is a powerful tool to understand liver embryonic 
      development, healthy liver cell homeostasis, tissue repair, and regeneration. 
      Lineage tracing of biliary epithelial cells (BECs) in the adult liver has been 
      used to assess the contribution of the biliary epithelium to liver injury, 
      regeneration, and disease. These studies have shown the contribution of BECs to 
      the expansion of ductular reaction (DR) and liver progenitor cells (LPCs) and 
      eventually the generation of new hepatocytes. Few genetic lineage-tracing mouse 
      models have been proved to trace BECs. This chapter is focused on lineage tracing 
      of BECs in mouse models of liver injury and regeneration. First, we mention 
      different existing approaches to trace the biliary epithelium based on proteins 
      specifically expressed by BECs such as sex-determining region Y-box 9 (SOX9), 
      osteopontin (OPN), and cytokeratin-19 (KRT19). Second, we describe mouse models 
      that can be used to evaluate cell fate during liver injury and regeneration 
      (i.e., partial hepatectomy (PHx), acute liver injury models, and chronic liver 
      damage models such as 3,5-diethoxycarbonyl-1,4-dihydro-collidin (DDC) diet, 
      choline-deficient ethionine-supplemented (CDE) diet, or chronic carbon 
      tetrachloride (CCl(4)) administration). Third, we suggest possible readouts to 
      assess BECs fate based on immunofluorescence analysis.
FAU - Rubio-Tomas, Teresa
AU  - Rubio-Tomas T
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, 
      Spain.
FAU - Aguilar-Bravo, Beatriz
AU  - Aguilar-Bravo B
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, 
      Spain.
FAU - Sancho-Bru, Pau
AU  - Sancho-Bru P
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, 
      Spain. psancho@clinic.cat.
AD  - Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBERehd), Barcelona, Spain. psancho@clinic.cat.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers)
RN  - 0 (Keratin-19)
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (Sox9 protein, mouse)
RN  - 0 (Spp1 protein, mouse)
RN  - 106441-73-0 (Osteopontin)
SB  - IM
MH  - Animals
MH  - Bile Ducts/*cytology/metabolism
MH  - Biomarkers/*metabolism
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Disease Models, Animal
MH  - Epithelial Cells/cytology/metabolism
MH  - Keratin-19/metabolism
MH  - Liver/cytology/metabolism
MH  - *Liver Regeneration
MH  - Lung Injury/etiology/*metabolism
MH  - Mice
MH  - Osteopontin/metabolism
MH  - SOX9 Transcription Factor/metabolism
OTO - NOTNLM
OT  - Animal models
OT  - Biliary epithelial cells
OT  - Chronic liver injury
OT  - Ductular reaction
OT  - Lineage tracing
OT  - Liver progenitor cells
OT  - Liver regeneration
EDAT- 2018/12/12 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/12/12 06:00
PHST- 2018/12/12 06:00 [entrez]
PHST- 2018/12/12 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
AID - 10.1007/978-1-4939-8961-4_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1905:45-57. doi: 10.1007/978-1-4939-8961-4_5.