PMID- 30536095
OWN - NLM
STAT- MEDLINE
DCOM- 20190611
LR  - 20190613
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1905
DP  - 2019
TI  - Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes 
      and Biliary Epithelial Cells.
PG  - 117-130
LID - 10.1007/978-1-4939-8961-4_11 [doi]
AB  - Bipotent liver progenitor cells (LPCs) are promising cell sources for cell 
      transplantation therapy in hepatic disorders as well as biliary dysfunctions. 
      Using a cocktail of small molecules, we recently reported a novel approach to 
      generate bipotent LPCs, named chemically induced liver progenitors (CLiPs), from 
      adult rat hepatocytes. In this chapter, we describe a detailed protocol for the 
      induction of rat CLiPs. We first describe the method to isolate primary rat 
      hepatocytes and then describe how to induce CLiPs from the hepatocytes. In 
      addition, we describe methods to induce the generated CLiPs to differentiate into 
      hepatocytes and biliary epithelial cells.
FAU - Katsuda, Takeshi
AU  - Katsuda T
AD  - Division of Molecular and Cellular Medicine, National Cancer Center Research 
      Institute, Tokyo, Japan.
FAU - Ochiya, Takahiro
AU  - Ochiya T
AD  - Division of Molecular and Cellular Medicine, National Cancer Center Research 
      Institute, Tokyo, Japan. tochiya@ncc.go.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 106956-32-5 (Oncostatin M)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Biliary Tract/cytology
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cellular Reprogramming
MH  - Dexamethasone/*pharmacology
MH  - Epithelial Cells/cytology
MH  - Female
MH  - Hepatocytes/*cytology/drug effects
MH  - Male
MH  - Oncostatin M/*pharmacology
MH  - Rats
MH  - Stem Cells/*cytology
OTO - NOTNLM
OT  - Biliary epithelial cell
OT  - Bipotentiality
OT  - CLiP
OT  - Hepatocyte
OT  - Liver progenitor cell
OT  - Reprogramming
EDAT- 2018/12/12 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/12/12 06:00
PHST- 2018/12/12 06:00 [entrez]
PHST- 2018/12/12 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
AID - 10.1007/978-1-4939-8961-4_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1905:117-130. doi: 10.1007/978-1-4939-8961-4_11.