PMID- 30537041
OWN - NLM
STAT- MEDLINE
DCOM- 20200227
LR  - 20231213
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Linking)
VI  - 222
IP  - 2
DP  - 2019 Apr
TI  - A small cysteine-rich protein from two kingdoms of microbes is recognized as a 
      novel pathogen-associated molecular pattern.
PG  - 995-1011
LID - 10.1111/nph.15631 [doi]
AB  - Pathogen-associated molecular patterns (PAMPs) are conserved molecules that are 
      crucial for normal life cycle of microorganisms. However, the diversity of 
      microbial PAMPs is little known. During screening of cell-death-inducing factors 
      from the necrotrophic fungus Valsa mali, we identified a novel PAMP VmE02 that is 
      widely spread in oomycetes and fungi. Agrobacterium tumefaciens-mediated 
      transient expression or infiltration of recombinant protein produced by 
      Escherichia coli was performed to assay elicitor activity of the proteins tested. 
      Virus-induced gene silencing in Nicotiana benthamiana was used to determine the 
      components involved in VmE02-triggered cell death. The role of VmE02 in virulence 
      and conidiation of V. mali were characterized by gene deletion and 
      complementation. We found that VmE02, together with some of its homologues from 
      both oomycete and fungal species, exhibited cell-death-inducing activity in 
      N. benthamiana. VmE02-triggered cell death was shown to be dependent on 
      BRI1-ASSOCIATED KINASE-1, SUPPRESSOR OF BIR1-1, HSP90 and SGT1 in N. benthamiana. 
      Deletion of VmE02 in V. mali greatly attenuated pathogen conidiation but not 
      virulence, and treatment of N. benthamiana with VmE02 enhances plant resistance 
      to Sclerotinia sclerotiorum and Phytophthora capsici. We conclude that VmE02 is a 
      novel cross-kingdom PAMP produced by several fungi and oomycetes.
CI  - (c) 2018 The Authors. New Phytologist (c) 2018 New Phytologist Trust.
FAU - Nie, Jiajun
AU  - Nie J
AUID- ORCID: 0000-0002-5765-0931
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Yin, Zhiyuan
AU  - Yin Z
AUID- ORCID: 0000-0002-7189-895X
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Li, Zhengpeng
AU  - Li Z
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Wu, Yuxing
AU  - Wu Y
AUID- ORCID: 0000-0001-5118-9964
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Huang, Lili
AU  - Huang L
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
LA  - eng
SI  - GENBANK/AF234298
SI  - GENBANK/KUI69068.1
SI  - GENBANK/XP_008893679.1
SI  - GENBANK/XP_008909123
SI  - GENBANK/XP_008904024
SI  - GENBANK/XP_664276.1
SI  - GENBANK/APA10122.1
SI  - GENBANK/XP_001592044
SI  - GENBANK/XP_001555760
SI  - GENBANK/KNF06837.1
SI  - GENBANK/KNE93445.1
SI  - GENBANK/KNE89953.1
GR  - 31471732/National Natural Science Foundation of China/International
GR  - 31671982/National Natural Science Foundation of China/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190108
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
RN  - 0 (Fungal Proteins)
RN  - 0 (Pathogen-Associated Molecular Pattern Molecules)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Ascomycota/metabolism
MH  - Cell Death
MH  - Fungal Proteins/*metabolism
MH  - Gene Expression Regulation, Plant
MH  - Oomycetes/metabolism
MH  - Pathogen-Associated Molecular Pattern Molecules/*metabolism
MH  - Phylogeny
MH  - Plant Immunity
MH  - Plant Proteins/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Spores, Fungal/physiology
MH  - Nicotiana/cytology/genetics/immunology/microbiology
OTO - NOTNLM
OT  - Nicotiana benthamiana
OT  - Valsa mali
OT  - cell death
OT  - filamentous pathogens
OT  - innate immunity
OT  - pathogen-associated molecular pattern (PAMP)
EDAT- 2018/12/12 06:00
MHDA- 2020/02/28 06:00
CRDT- 2018/12/12 06:00
PHST- 2018/08/18 00:00 [received]
PHST- 2018/12/01 00:00 [accepted]
PHST- 2018/12/12 06:00 [pubmed]
PHST- 2020/02/28 06:00 [medline]
PHST- 2018/12/12 06:00 [entrez]
AID - 10.1111/nph.15631 [doi]
PST - ppublish
SO  - New Phytol. 2019 Apr;222(2):995-1011. doi: 10.1111/nph.15631. Epub 2019 Jan 8.