PMID- 30538428
OWN - NLM
STAT- MEDLINE
DCOM- 20190326
LR  - 20220330
IS  - 1177-8881 (Electronic)
IS  - 1177-8881 (Linking)
VI  - 12
DP  - 2018
TI  - Icariin promotes the migration of bone marrow stromal cells via the 
      SDF-1alpha/HIF-1alpha/CXCR4 pathway.
PG  - 4023-4031
LID - 10.2147/DDDT.S179989 [doi]
AB  - PURPOSE: In this study, a series of in vitro experiments were performed to 
      investigate the molecular mechanisms underlying cell migration promoted by 
      icariin (ICA) at low concentrations. MATERIALS AND METHODS: Bone marrow stromal 
      cells (BMSCs) were cultured with different concentrations of ICA to verify 
      whether it can enhance the efficiency of BMSCs migration. Western blot was 
      employed to measure the expression of hypoxia-inducible factor-1alpha (HIF-1alpha) and 
      C-X-C chemokine receptor type 4 (CXCR4) at different time points in BMSCs treated 
      with ICA. Subsequently, we evaluated the function of HIF-1alpha in the expression of 
      CXCR4 and the migration of cells by transfecting plasmid HIF-1alpha small interfering 
      RNA (siHIF-1alpha) into BMSCs model. RESULTS: Our data indicated that different 
      concentrations of ICA (10, 1, and 0.1 microM) further enhanced the chemotactic 
      capability of SDF-1alpha, and the most prominent cell migration stimulatory effect 
      was observed with 1 microM ICA. Furthermore, ICA significantly enhanced the protein 
      levels of CXCR4 and HIF-1alpha, and this effect was blocked by ICI 12,780 (estrogen 
      receptor antagonis). Moreover, transfection of BMSCs with siHIF-1alpha reduced CXCR4 
      expression, suggesting that HIF-1alpha can regulate the migration of cells by 
      influencing the expression of CXCR4. CONCLUSION: ICA promoted BMSCs migration via 
      the activation of HIF-1alpha and further regulated the expression of CXCR4, 
      suggesting that ICA might have beneficial effects in stem cell therapy.
FAU - Zhu, Haiyan
AU  - Zhu H
AD  - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of 
      Stomatology, Shandong University, Jinan, Shandong Province, China, 
      zhangj@sdu.edu.cn.
AD  - Department of Orthodontics, School of Stomatology, Shandong University, Jinan, 
      Shandong Province, China, zhangj@sdu.edu.cn.
AD  - Department of Stomatology, Weihai Municipal Hospital, Weihai, Shandong Province, 
      China.
FAU - Wang, Xuxia
AU  - Wang X
AD  - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of 
      Stomatology, Shandong University, Jinan, Shandong Province, China, 
      zhangj@sdu.edu.cn.
AD  - Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong 
      University, Jinan, Shandong Province, China.
FAU - Han, Yuanyuan
AU  - Han Y
AD  - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of 
      Stomatology, Shandong University, Jinan, Shandong Province, China, 
      zhangj@sdu.edu.cn.
AD  - Department of Orthodontics, School of Stomatology, Shandong University, Jinan, 
      Shandong Province, China, zhangj@sdu.edu.cn.
FAU - Zhang, Wenjuan
AU  - Zhang W
AD  - Department of Stomatology, Affiliated Hospital of Shandong University of 
      Traditional Chinese Medicine, Jinan, Shandong Province, China.
FAU - Xin, Wei
AU  - Xin W
AD  - Department of Stomatology, Weihai Municipal Hospital, Weihai, Shandong Province, 
      China.
FAU - Zheng, Xiaotao
AU  - Zheng X
AD  - Department of Stomatology, Weihai Municipal Hospital, Weihai, Shandong Province, 
      China.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of 
      Stomatology, Shandong University, Jinan, Shandong Province, China, 
      zhangj@sdu.edu.cn.
AD  - Department of Orthodontics, School of Stomatology, Shandong University, Jinan, 
      Shandong Province, China, zhangj@sdu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20181122
PL  - New Zealand
TA  - Drug Des Devel Ther
JT  - Drug design, development and therapy
JID - 101475745
RN  - 0 (CXCL12 protein, rat)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcr4 protein, rat)
RN  - 0 (Flavonoids)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Receptors, CXCR4)
RN  - VNM47R2QSQ (icariin)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects/metabolism
MH  - Cell Movement/*drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chemokine CXCL12/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Endoplasmic Reticulum/drug effects/metabolism
MH  - Flavonoids/*pharmacology
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, CXCR4/*metabolism
MH  - Stromal Cells/cytology/*drug effects/metabolism
MH  - Structure-Activity Relationship
PMC - PMC6254989
OTO - NOTNLM
OT  - CXCR4
OT  - HIF-1alpha
OT  - SDF-1alpha
OT  - cell migration
OT  - icariin
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2018/12/13 06:00
MHDA- 2019/03/27 06:00
PMCR- 2018/11/22
CRDT- 2018/12/13 06:00
PHST- 2018/12/13 06:00 [entrez]
PHST- 2018/12/13 06:00 [pubmed]
PHST- 2019/03/27 06:00 [medline]
PHST- 2018/11/22 00:00 [pmc-release]
AID - dddt-12-4023 [pii]
AID - 10.2147/DDDT.S179989 [doi]
PST - epublish
SO  - Drug Des Devel Ther. 2018 Nov 22;12:4023-4031. doi: 10.2147/DDDT.S179989. 
      eCollection 2018.