PMID- 30538464
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20220330
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 13
DP  - 2018
TI  - Targeted delivery of reduced graphene oxide nanosheets using multifunctional 
      ultrasound nanobubbles for visualization and enhanced photothermal therapy.
PG  - 7859-7872
LID - 10.2147/IJN.S181268 [doi]
AB  - Ultrasound molecular imaging as a promising strategy, which involved the use of 
      molecularly targeted contrast agents, combined the advantages of 
      contrast-enhanced ultrasound with the photothermal effect of reduced graphene 
      oxide (rGO). METHODS AND RESULTS: The heparin sulfate proteoglycan glypican-3 
      (GPC3) is a potential molecular target for hepatocellular carcinoma (HCC). In 
      this study, we covalently linked biotinylated GPC3 antibody to PEGylated nano-rGO 
      to obtain GPC3-modified rGO-PEG (rGO-GPC3), and then combined rGO-GPC3 with 
      avidinylated nanobubbles (NBs) using biotin-avidin system to prepare NBs-GPC3-rGO 
      with photothermal effect and dispersibility, solubility in physiological 
      environment. The average size of NBs-GPC3-rGO complex was 700.4+/-52.9 nm due to 
      the polymerization of biotin-avidin system. Scanning electron microscope (SEM) 
      showed NBs-GPC3-rGO attached to human hepatocellular carcinoma HepG2 cell. The 
      ultrasound-targeted nanobubble destruction (UTND) technology make use of the 
      physical energy of ultrasound exposure for the improvement of rGO delivery. 
      Compared with other control groups, the highest nanobubble destruction efficiency 
      of NBs-GPC3-rGO was attributed to the dissection effect of rGO on UTND. This is a 
      positive feedback effect that leads to an increase in the concentration of rGO 
      around the HepG2 cell. So NBs-GPC3-rGO using UTND and near-infrared (NIR) 
      irradiation resulted in cell viability within 24 h, 48 h, 72 h lower than other 
      treatment groups. CONCLUSION: This work established NBs-GPC3-rGO as an ultrasonic 
      photothermal agent due to its suitable size, imaging capability, photothermal 
      efficiency for visual photothermal therapy in vitro.
FAU - Liu, Zhao
AU  - Liu Z
AD  - Department of Ultrasound, Harbin Medical University Cancer Hospital, Nangang 
      District, Harbin 150080, China, chengwen69@yahoo.com.
FAU - Zhang, Jia
AU  - Zhang J
AD  - Key Laboratory of Microsystems and Microstructure Manufacturing, Ministry of 
      Education, Harbin Institute of Technology, Harbin 150080, China.
FAU - Tian, Yuhang
AU  - Tian Y
AD  - Department of Ultrasound, Harbin Medical University Cancer Hospital, Nangang 
      District, Harbin 150080, China, chengwen69@yahoo.com.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Department of Ultrasound, Harbin Medical University Cancer Hospital, Nangang 
      District, Harbin 150080, China, chengwen69@yahoo.com.
FAU - Han, Xue
AU  - Han X
AD  - Department of Ultrasound, Harbin Medical University Cancer Hospital, Nangang 
      District, Harbin 150080, China, chengwen69@yahoo.com.
FAU - Wang, Qiucheng
AU  - Wang Q
AD  - Department of Ultrasound, Harbin Medical University Cancer Hospital, Nangang 
      District, Harbin 150080, China, chengwen69@yahoo.com.
FAU - Cheng, Wen
AU  - Cheng W
AD  - Department of Ultrasound, Harbin Medical University Cancer Hospital, Nangang 
      District, Harbin 150080, China, chengwen69@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20181122
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Glypicans)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
SB  - IM
EIN - Int J Nanomedicine. 2019 Apr 08;14:2449-2450. PMID: 31040667
MH  - Cell Survival
MH  - Glypicans/metabolism
MH  - Graphite/*chemistry
MH  - Hep G2 Cells
MH  - Humans
MH  - Hyperthermia, Induced/*methods
MH  - *Microbubbles
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Oxidation-Reduction
MH  - Phototherapy/*methods
MH  - *Ultrasonics
PMC - PMC6255282
OTO - NOTNLM
OT  - HepG2 cell
OT  - glypican-3
OT  - photothermal therapy
OT  - reduced graphene oxide
OT  - ultrasound-targeted nanobubble destruction
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2018/12/13 06:00
MHDA- 2019/01/15 06:00
PMCR- 2018/11/22
CRDT- 2018/12/13 06:00
PHST- 2018/12/13 06:00 [entrez]
PHST- 2018/12/13 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
PHST- 2018/11/22 00:00 [pmc-release]
AID - ijn-13-7859 [pii]
AID - 10.2147/IJN.S181268 [doi]
PST - epublish
SO  - Int J Nanomedicine. 2018 Nov 22;13:7859-7872. doi: 10.2147/IJN.S181268. 
      eCollection 2018.