PMID- 30539484
OWN - NLM
STAT- MEDLINE
DCOM- 20190611
LR  - 20190613
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1904
DP  - 2019
TI  - A Cell-Based Reporter Assay Measuring the Activation of Fc Gamma Receptors 
      Induced by Therapeutic Monoclonal Antibodies.
PG  - 423-429
LID - 10.1007/978-1-4939-8958-4_21 [doi]
AB  - Fc gamma receptors (FcgammaRs) are expressed on the surface of various immune cells, 
      and the interactions between FcgammaRs and the Fc region of immunoglobulin G are 
      involved in the activation of immune cells by antigen-bound antibodies. 
      Fc-mediated immune-cell activations are related to both the efficacy and the 
      safety of therapeutic monoclonal antibodies. It is indispensable to elucidate the 
      Fc-mediated functions in the development of therapeutic monoclonal antibodies. 
      Here, we describe a cell-based assay using FcgammaR-expressing reporter cell lines 
      that can be used to evaluate the human FcgammaR-activation properties of therapeutic 
      monoclonal antibodies by a rapid and simple procedure.
FAU - Aoyama, Michihiko
AU  - Aoyama M
AD  - Division of Biological Chemistry and Biologicals, National Institute of Health 
      Sciences, Kawasaki, Kanagawa, Japan.
FAU - Tada, Minoru
AU  - Tada M
AD  - Division of Biological Chemistry and Biologicals, National Institute of Health 
      Sciences, Kawasaki, Kanagawa, Japan. m-tada@nihs.go.jp.
FAU - Ishii-Watabe, Akiko
AU  - Ishii-Watabe A
AD  - Division of Biological Chemistry and Biologicals, National Institute of Health 
      Sciences, Kawasaki, Kanagawa, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Antibodies, Monoclonal/immunology/*pharmacology
MH  - *Biological Assay/methods
MH  - Cell Line
MH  - Gene Expression
MH  - Genes, Reporter
MH  - Humans
MH  - Immunoglobulin Fc Fragments/immunology
MH  - Immunoglobulin G/immunology/*pharmacology
MH  - Receptors, IgG/*agonists
OTO - NOTNLM
OT  - Antibody Fc region
OT  - Cell-based reporter assay
OT  - Effector function
OT  - Fc gamma receptor
OT  - Therapeutic monoclonal antibody
EDAT- 2018/12/13 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/12/13 06:00
PHST- 2018/12/13 06:00 [entrez]
PHST- 2018/12/13 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
AID - 10.1007/978-1-4939-8958-4_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1904:423-429. doi: 10.1007/978-1-4939-8958-4_21.