PMID- 30541650
OWN - NLM
STAT- MEDLINE
DCOM- 20190308
LR  - 20190308
IS  - 2095-4352 (Print)
VI  - 30
IP  - 11
DP  - 2018 Nov
TI  - [Epinephrine combined with acupuncture therapy can treat anaphylactic shock mice 
      through nuclear factor-kappaB signaling pathway: a rat experimental result].
PG  - 1083-1086
LID - 10.3760/cma.j.issn.2095-4352.2018.011.014 [doi]
AB  - OBJECTIVE: To explore the therapeutic effect of epinephrine combined with 
      acupuncture on anaphylactic shock and its mechanism. METHODS: Sixty male Kunming 
      mice were randomly divided into normal saline (NS) group, anaphylactic shock 
      model group, and integrated traditional Chinese and Western medicine treatment 
      group with 20 mice in each group. The anaphylactic shock model was reproduced by 
      egg albumin infusion: intraperitoneal injection of 0.25 mL egg albumin (0.01 
      mmol/L), repeated injection 1 week later, and intravenous injection of 0.5 mL egg 
      albumin through caudal vein on the 3rd week to induce anaphylactic shock. The 
      mice in the NS group were injected with NS. The mice in the treatment group were 
      immediately subcutaneously injected with 0.2 mug of 0.1% epinephrine, and 
      intraperitoneally injected with aminophylline 0.2 mg, combined with acupuncture 
      at Shuigou, Neiguan and Hegu points. Number of died mice in each group were 
      observed at 1, 6, and 12 hours after model reproduction. The mice were sacrificed 
      at 12 hours, the blood was harvested, and the serum tryptase, immunoglobulin E 
      (IgE), tumor necrosis factor-alpha (TNF-alpha), interleukins (IL-1 and IL-6) were 
      determined by enzyme linked immunosorbent assay (ELISA). The lung tissues were 
      harvested, and the protein expressions of p65, phosphorylation of p65 (p-p65), 
      and phosphorylation of nuclear factor-kappaB inhibitor alpha (p-IkappaBalpha) were determined by 
      Western Blot. RESULTS: No mice died in the NS control group at 12 hours. In the 
      treatment group, the mortality at 12 hours was significantly lower than that in 
      the model group (10% vs. 80%, P < 0.01). The levels of tryptase and IgE in the 
      model group were significantly higher than those in the NS control group 
      [tryptase (mug/L): 1.53+/-0.28 vs. 0.91+/-0.23, IgE (mug/L): 33.3+/-3.1 vs. 21.3+/-1.9, 
      both P < 0.01], both levels in the treatment group were significantly lower than 
      those in the model group [tryptase (mug/L): 1.31+/-0.26 vs. 1.53+/-0.28, IgE (mug/L): 
      25.6+/-2.2 vs. 33.3+/-3.1, both P < 0.05]. The levels of TNF-alpha, IL-1 and IL-6 in the 
      model group were significantly higher than those in the NS control group [TNF-alpha 
      (ng/L): 35.3+/-4.7 vs. 16.4+/-3.5, IL-1 (ng/L): 13.8+/-3.3 vs. 4.2+/-1.8, IL-6 (ng/L): 
      15.3+/-4.8 vs. 5.5+/-2.1, all P < 0.01]. The serum inflammatory factors of the 
      treatment group were significantly higher than those of the model group [TNF-alpha 
      (ng/L): 26.1+/-4.3 vs. 35.3+/-4.7, IL-1 (ng/L): 7.2+/-2.7 vs. 13.8+/-3.3, IL-6 (ng/L): 
      8.8+/-3.8 vs. 15.3+/-4.8, all P < 0.05]. It was shown by Western Blot results that 
      there was no significant difference in p65 protein expression of the lung tissue 
      among the three groups. In the NS control group, the expression of p-p65 protein 
      in the nucleus of the lung tissue was extremely low but was significantly 
      increased in the model group, and p-p65 protein in the treatment group was 
      significantly decreased as compared with that in the model group. The expression 
      tendency of p-IBalpha protein was consistent with that of p-p65. CONCLUSIONS: 
      Epinephrine combined with acupuncture plays a therapeutic role in mice with 
      anaphylactic shock by inhibiting the activation of NF-kappaB signaling pathway.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Department of Dermatology, Xinxiang Central Hospital, Xinxiang 453000, Henan, 
      China. Corresponding author: Gan Caibin, Email: gancaibinzx@163.com.
FAU - Gan, Caibin
AU  - Gan C
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhonghua wei zhong bing ji jiu yi xue
JID - 101604552
RN  - 0 (NF-kappa B)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - *Acupuncture Therapy
MH  - Anaphylaxis/*therapy
MH  - Animals
MH  - Combined Modality Therapy
MH  - Epinephrine/*therapeutic use
MH  - Male
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Rats
MH  - Signal Transduction/drug effects
EDAT- 2018/12/14 06:00
MHDA- 2019/03/09 06:00
CRDT- 2018/12/14 06:00
PHST- 2018/12/14 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/03/09 06:00 [medline]
AID - 10.3760/cma.j.issn.2095-4352.2018.011.014 [doi]
PST - ppublish
SO  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2018 Nov;30(11):1083-1086. doi: 
      10.3760/cma.j.issn.2095-4352.2018.011.014.