PMID- 30541955
OWN - NLM
STAT- MEDLINE
DCOM- 20190129
LR  - 20211204
IS  - 0973-7138 (Electronic)
IS  - 0250-5991 (Linking)
VI  - 43
IP  - 5
DP  - 2018 Dec
TI  - Classical swine fever virus non-structural protein 4B binds tank-binding kinase 
      1.
PG  - 947-957
AB  - Classical swine fever (CSF) is a contagious disease with a high mortality rate 
      and is caused by classical swine fever virus (CSFV). CSFV non-structural protein 
      4B (NS4B) plays a crucial role in CSFV replication and pathogenicity. However, 
      precisely how NS4B exerts these functions remains unknown, especially as there 
      are no reports relating to potential cellular partners of CSFV NS4B. Here, a 
      yeast two-hybrid (Y2H) system was used to screen the cellular proteins 
      interacting with NS4B from a porcine alveolar macrophage (PAM) cDNA library. The 
      protein screen along with alignment using the NCBI database revealed 14 cellular 
      proteins that interact with NS4B: DDX39B, COX7C, FTH1, MAVS, NR2F6, RPLP1, PSMC4, 
      FGL2, MKRN1, RPL15, RPS3, RAB22A, TP53BP2 and TBK1. These proteins mostly relate 
      to oxidoreductase activity, signal transduction, localization, biological 
      regulation, catalytic activity, transport and metabolism by GO categories. 
      Tank-binding kinase 1 (TBK1) was chosen for further confirmation. The NS4B-TBK1 
      interaction was further confirmed by subcellular co-location, 
      co-immunoprecipitation and glutathione S-transferase pull-down assays. This study 
      offers a theoretical foundation for further understanding of the diversity of 
      NS4B functions in relation to viral infection and subsequent pathogenesis.
FAU - Lv, Huifang
AU  - Lv H
AD  - College of Veterinary Medicine, Northwest A and F University, No. 22 Xinong Road, 
      Yangling 712100, Shaanxi, China.
FAU - Dong, Wang
AU  - Dong W
FAU - Cao, Zhi
AU  - Cao Z
FAU - Lin, Jihui
AU  - Lin J
FAU - Ouyang, Yueling
AU  - Ouyang Y
FAU - Guo, Kangkang
AU  - Guo K
FAU - Li, Cunfa
AU  - Li C
FAU - Zhang, Yanming
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Biosci
JT  - Journal of biosciences
JID - 8100809
RN  - 0 (NS4B protein, flavivirus)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Classical Swine Fever Virus/*genetics/metabolism
MH  - *Gene Expression Regulation
MH  - Gene Library
MH  - Gene Ontology
MH  - *Host-Pathogen Interactions
MH  - Immunoprecipitation
MH  - Macrophages, Alveolar/metabolism/*virology
MH  - Molecular Sequence Annotation
MH  - Plasmids/chemistry/metabolism
MH  - Protein Binding
MH  - Protein Serine-Threonine Kinases/*genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics/metabolism
MH  - Signal Transduction
MH  - Swine
MH  - Two-Hybrid System Techniques
MH  - Viral Nonstructural Proteins/*genetics/metabolism
EDAT- 2018/12/14 06:00
MHDA- 2019/01/30 06:00
CRDT- 2018/12/14 06:00
PHST- 2018/12/14 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/01/30 06:00 [medline]
PST - ppublish
SO  - J Biosci. 2018 Dec;43(5):947-957.