PMID- 30542361
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231004
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 9
DP  - 2018
TI  - Role for Arabidopsis PLC7 in Stomatal Movement, Seed Mucilage Attachment, and 
      Leaf Serration.
PG  - 1721
LID - 10.3389/fpls.2018.01721 [doi]
LID - 1721
AB  - Phospholipase C (PLC) has been suggested to play important roles in plant stress 
      and development. To increase our understanding of PLC signaling in plants, we 
      have started to analyze knock-out (KO), knock-down (KD) and overexpression 
      mutants of Arabidopsis thaliana, which contains nine PLCs. Earlier, we 
      characterized PLC2, PLC3 and PLC5. Here, the role of PLC7 is functionally 
      addressed. Promoter-GUS analyses revealed that PLC7 is specifically expressed in 
      the phloem of roots, leaves and flowers, and is also present in trichomes and 
      hydathodes. Two T-DNA insertion mutants were obtained, i.e., plc7-3 being a KO- 
      and plc7-4 a KD line. In contrast to earlier characterized phloem-expressed PLC 
      mutants, i.e., plc3 and plc5, no defects in primary- or lateral root development 
      were found for plc7 mutants. Like plc3 mutants, they were less sensitive to ABA 
      during stomatal closure. Double-knockout plc3 plc7 lines were lethal, but plc5 
      plc7 (plc5/7) double mutants were viable, and revealed several new phenotypes, 
      not observed earlier in the single mutants. These include a defect in seed 
      mucilage, enhanced leaf serration, and an increased tolerance to drought. 
      Overexpression of PLC7 enhanced drought tolerance too, similar to what was 
      earlier found for PLC3-and PLC5 overexpression. In vivo (32)P(i)-labeling of 
      seedlings and treatment with sorbitol to mimic drought stress, revealed stronger 
      PIP(2) responses in both drought-tolerant plc5/7 and PLC7-OE mutants. Together, 
      these results show novel functions for PLC in plant stress and development. 
      Potential molecular mechanisms are discussed.
FAU - van Wijk, Ringo
AU  - van Wijk R
AD  - Section Plant Physiology, University of Amsterdam, Amsterdam, Netherlands.
AD  - Section Plant Cell Biology, Swammerdam Institute for Life Sciences (SILS), 
      University of Amsterdam, Amsterdam, Netherlands.
FAU - Zhang, Qianqian
AU  - Zhang Q
AD  - Section Plant Physiology, University of Amsterdam, Amsterdam, Netherlands.
AD  - Section Plant Cell Biology, Swammerdam Institute for Life Sciences (SILS), 
      University of Amsterdam, Amsterdam, Netherlands.
FAU - Zarza, Xavier
AU  - Zarza X
AD  - Section Plant Physiology, University of Amsterdam, Amsterdam, Netherlands.
AD  - Section Plant Cell Biology, Swammerdam Institute for Life Sciences (SILS), 
      University of Amsterdam, Amsterdam, Netherlands.
FAU - Lamers, Mart
AU  - Lamers M
AD  - Section Plant Physiology, University of Amsterdam, Amsterdam, Netherlands.
FAU - Marquez, Francisca Reyes
AU  - Marquez FR
AD  - Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, 
      Netherlands.
FAU - Guardia, Aisha
AU  - Guardia A
AD  - Instituto de Investigaciones Biologicas (IIB-CONICET-UNMdP), Universidad Nacional 
      de Mar del Plata, Mar del Plata, Argentina.
FAU - Scuffi, Denise
AU  - Scuffi D
AD  - Instituto de Investigaciones Biologicas (IIB-CONICET-UNMdP), Universidad Nacional 
      de Mar del Plata, Mar del Plata, Argentina.
FAU - Garcia-Mata, Carlos
AU  - Garcia-Mata C
AD  - Instituto de Investigaciones Biologicas (IIB-CONICET-UNMdP), Universidad Nacional 
      de Mar del Plata, Mar del Plata, Argentina.
FAU - Ligterink, Wilco
AU  - Ligterink W
AD  - Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, 
      Netherlands.
FAU - Haring, Michel A
AU  - Haring MA
AD  - Section Plant Physiology, University of Amsterdam, Amsterdam, Netherlands.
FAU - Laxalt, Ana M
AU  - Laxalt AM
AD  - Instituto de Investigaciones Biologicas (IIB-CONICET-UNMdP), Universidad Nacional 
      de Mar del Plata, Mar del Plata, Argentina.
FAU - Munnik, Teun
AU  - Munnik T
AD  - Section Plant Physiology, University of Amsterdam, Amsterdam, Netherlands.
AD  - Section Plant Cell Biology, Swammerdam Institute for Life Sciences (SILS), 
      University of Amsterdam, Amsterdam, Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20181127
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC6278229
OTO - NOTNLM
OT  - ABA sensitivity
OT  - PLC
OT  - drought tolerance
OT  - leaf serration
OT  - seed mucilage
EDAT- 2018/12/14 06:00
MHDA- 2018/12/14 06:01
PMCR- 2018/01/01
CRDT- 2018/12/14 06:00
PHST- 2018/09/24 00:00 [received]
PHST- 2018/11/05 00:00 [accepted]
PHST- 2018/12/14 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2018/12/14 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2018.01721 [doi]
PST - epublish
SO  - Front Plant Sci. 2018 Nov 27;9:1721. doi: 10.3389/fpls.2018.01721. eCollection 
      2018.