PMID- 30542493
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 16
IP  - 6
DP  - 2018 Dec
TI  - Protective effect of avicularin on rheumatoid arthritis and its associated 
      mechanisms.
PG  - 5343-5349
LID - 10.3892/etm.2018.6872 [doi]
AB  - The present study aimed to investigate the effect of avicularin on rheumatoid 
      arthritis (RA) in vitro, and additionally explore the molecular mechanism. To 
      perform this investigation, an in vitro model of RA was established by treatment 
      of the human RA synovial MH7A cell line with tumor necrosis factor-alpha (TNF-alpha). 
      MH7A cells were then treated with various concentrations (10, 30, 100 and 300 microM) 
      of avicularin. Then, the levels of inflammatory factors [interleukin (IL)-1beta, 
      IL-6, IL-8, matrix metalloproteinase (MMP)-1 and MMP-13] were measured by ELISA. 
      Cell viability and apoptosis were detected using an MTT assay and flow cytometry, 
      respectively. In addition, the expression levels of genes and proteins were 
      determined reverse transcription quantitative polymerase chain reaction and 
      western blot analysis. The results of the present study indicated that avicularin 
      significantly decreased the levels of inflammatory factors (IL-1beta, IL-6, IL-8, 
      MMP-1 and MMP-13), previously increased by TNF-alpha, in a dose-dependent manner. 
      Concurrently, avicularin inhibited the mRNA and protein expression levels of iNOS 
      and COX-2 increased by TNF-alpha. It was also identified that TNF-alpha administration 
      significantly promoted MH7A cell viability and inhibited cell apoptosis, and 
      avicularin treatment dose-dependently inhibited MH7A cell viability and induced 
      cell apoptosis. In addition, these data suggested that avicularin prevented the 
      activation of the mitogen-activated protein kinase kinase (MEK)/nuclear factor 
      kappa light-chain-enhancer of activated B-cells (NF-kappaB) pathway activated by 
      TNF-alpha. Taken together, these results demonstrated that avicularin may inhibit the 
      inflammatory response, prevent cell viability and induce apoptosis in human RA 
      synovial cells through preventing the activation of the MEK/NF-kappaB pathway.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Rheumatology and Nephrology, Hangzhou Red Cross Hospital, Hangzhou, 
      Zhejiang 310003, P.R. China.
FAU - Zheng, Hongxia
AU  - Zheng H
AD  - Department of Rheumatology and Nephrology, Hangzhou Red Cross Hospital, Hangzhou, 
      Zhejiang 310003, P.R. China.
FAU - Zheng, Minwei
AU  - Zheng M
AD  - Department of Rheumatology and Nephrology, Hangzhou Red Cross Hospital, Hangzhou, 
      Zhejiang 310003, P.R. China.
FAU - Liu, Xiaoxian
AU  - Liu X
AD  - Department of Rheumatology and Nephrology, Hangzhou Red Cross Hospital, Hangzhou, 
      Zhejiang 310003, P.R. China.
FAU - Yu, Jianning
AU  - Yu J
AD  - Department of Rheumatology and Nephrology, Hangzhou Red Cross Hospital, Hangzhou, 
      Zhejiang 310003, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20181017
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6257287
OTO - NOTNLM
OT  - avicularin
OT  - inflammatory response
OT  - rheumatoid arthritis
OT  - synovial fibroblasts
EDAT- 2018/12/14 06:00
MHDA- 2018/12/14 06:01
PMCR- 2018/10/17
CRDT- 2018/12/14 06:00
PHST- 2018/05/17 00:00 [received]
PHST- 2018/08/31 00:00 [accepted]
PHST- 2018/12/14 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2018/12/14 06:01 [medline]
PHST- 2018/10/17 00:00 [pmc-release]
AID - ETM-0-0-6872 [pii]
AID - 10.3892/etm.2018.6872 [doi]
PST - ppublish
SO  - Exp Ther Med. 2018 Dec;16(6):5343-5349. doi: 10.3892/etm.2018.6872. Epub 2018 Oct 
      17.