PMID- 30543090
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1303-6165 (Electronic)
IS  - 1300-0144 (Linking)
VI  - 48
IP  - 6
DP  - 2018 Dec 12
TI  - Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the 
      identification of mycobacteria species.
PG  - 1351-1357
LID - 10.3906/sag-1801-198 [doi]
AB  - BACKGROUND/AIM: Matrix-assisted laser desorption/ionization time-of-flight 
      (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria 
      via the analysis of biomolecules. It is being increasingly used in routine 
      microbiology practice since it permits early, rapid, and cost-effective 
      identification of pathogens of clinical importance. In this study, we aimed to 
      evaluate the efficacy of phenotypic identification of mycobacteria by the 
      MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to 
      standard sequence analysis. MATERIALS AND METHODS: A total of 155 Mycobacterium 
      clinical and external quality control isolates, comprising nontuberculous 
      mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 
      60), were included in the study. RESULTS: Identification by MBT ML4.0 was 
      correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC 
      isolates were correctly differentiated from NTM isolates. CONCLUSION: Based on 
      our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.
CI  - This work is licensed under a Creative Commons Attribution 4.0 International 
      License.
FAU - Akyar, Isin
AU  - Akyar I
AUID- ORCID: 0000-0003-1115-0429
AD  - Department of Medical Microbiology, School of Medicine, Acibadem University, 
      Istanbul, Turkey
AD  - Acibadem Labmed Medical Laboratories, Istanbul, Turkey
FAU - Cavusoglu, Cengiz
AU  - Cavusoglu C
AUID- ORCID: 0000-0001-7152-5216
AD  - Department of Medical Microbiology, School of Medicine, Ege University, Izmir, 
      Turkey
FAU - Ayas, Meltem
AU  - Ayas M
AUID- ORCID: 0000-0003-1920-9261
AD  - Department of Medical Microbiology, School of Medicine, Acibadem University, 
      Istanbul, Turkey
FAU - Surucuoglu, Suheyla
AU  - Surucuoglu S
AUID- ORCID: 0000-0001-8545-3364
AD  - Department of Medical Microbiology, School of Medicine, Celal Bayar University, 
      Manisa, Turkey
FAU - Ilki, Arzu
AU  - Ilki A
AUID- ORCID: 0000-0003-3887-7003
AD  - Department of Medical Microbiology, School of Medicine, Marmara University, 
      Istanbul, Turkey
FAU - Kaya, Deniz Ece
AU  - Kaya DE
AUID- ORCID: 0000-0002-7037-2497
AD  - Department of Medical Microbiology, School of Medicine, Acibadem University, 
      Istanbul, Turkey
FAU - Besli, Yesim
AU  - Besli Y
AUID- ORCID: 0000-0003-4574-6036
AD  - Department of Medical Microbiology, School of Medicine, Acibadem University, 
      Istanbul, Turkey
LA  - eng
PT  - Journal Article
DEP - 20181212
PL  - Turkey
TA  - Turk J Med Sci
JT  - Turkish journal of medical sciences
JID - 9441758
OTO - NOTNLM
OT  - *MALDI-TOF MS
OT  - *identification
OT  - *Mycobacterium
OT  - *Mycobacterium tuberculosis complex
OT  - *nontuberculous mycobacteria
EDAT- 2018/12/14 06:00
MHDA- 2018/12/14 06:01
CRDT- 2018/12/14 06:00
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2018/12/14 06:01 [medline]
PHST- 2018/12/14 06:00 [entrez]
AID - 10.3906/sag-1801-198 [doi]
PST - epublish
SO  - Turk J Med Sci. 2018 Dec 12;48(6):1351-1357. doi: 10.3906/sag-1801-198.