PMID- 30544157
OWN - NLM
STAT- MEDLINE
DCOM- 20190524
LR  - 20190524
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 51
IP  - 1
DP  - 2019 Jan 1
TI  - Short-term propamocarb exposure induces hepatic metabolism disorder associated 
      with gut microbiota dysbiosis in adult male zebrafish.
PG  - 88-96
LID - 10.1093/abbs/gmy153 [doi]
AB  - Propamocarb (PM) is a pesticide that is widely used to protect cucumbers and 
      other plants from downy mildew. Recently, some studies indicated that PM exposure 
      had potential toxic effects in animals. In this study, adult male zebrafish were 
      exposed to 100 and 1000 mug/l PM for 7 days to assess its effects on metabolism 
      and the gut microbiota. We observed a significant decrease in triglyceride (TG) 
      in the livers of zebrafish that were exposed to 1000 mug/l PM for 7 days. At the 
      same time, some genes related to glycolysis and lipid metabolism in the livers of 
      zebrafish, including hexokinase-1 (HK1), pyruvate kinase (PK), acyl-CoA oxidase 
      (Aco), peroxisome proliferator activated receptor alpha (Ppar-alpha), apolipoprotein 
      A-IV-like (Apo), Acetyl CoA carboxylase-1 (Acc1), diacylglycerol acyltransferase 
      (Dgat), and fatty acid synthase (Fas), were also decreased significantly after PM 
      exposure. Based on GC-MS metabolomics analysis, a total of 48 metabolites changed 
      significantly in the 1000 mug/l PM treatment group in comparison with the control 
      group. These altered metabolites were mainly associated with the glycolysis, 
      amino acid metabolism, and lipid metabolism pathways. Interestingly, we further 
      found that the 1000 mug/l PM treatment group also showed significant elevations in 
      Proteobacteria, Bacteroidetes, and Firmicutes at the phylum level. Sequencing of 
      the 16S rRNA gene in the V3-V4 region also showed a significant change in the 
      abundance and diversity of the gut microbiota in the 1000 mug/l PM treatment 
      group. Our results indicated that exposure to PM for a short time could induce 
      hepatic metabolic disorders and gut microbiota dysbiosis in adult male zebrafish.
FAU - Zhang, Rui
AU  - Zhang R
AD  - Department of Biotechnology, College of Biotechnology and Bioengineering, 
      Zhejiang University of Technology, Hangzhou, China.
FAU - Pan, Zihong
AU  - Pan Z
AD  - Department of Biotechnology, College of Biotechnology and Bioengineering, 
      Zhejiang University of Technology, Hangzhou, China.
FAU - Wang, Xiaoyu
AU  - Wang X
AD  - Department of Biotechnology, College of Biotechnology and Bioengineering, 
      Zhejiang University of Technology, Hangzhou, China.
FAU - Shen, Manlu
AU  - Shen M
AD  - Department of Biotechnology, College of Biotechnology and Bioengineering, 
      Zhejiang University of Technology, Hangzhou, China.
FAU - Zhou, Jiajie
AU  - Zhou J
AD  - Department of Biotechnology, College of Biotechnology and Bioengineering, 
      Zhejiang University of Technology, Hangzhou, China.
FAU - Fu, Zhengwei
AU  - Fu Z
AD  - Department of Biotechnology, College of Biotechnology and Bioengineering, 
      Zhejiang University of Technology, Hangzhou, China.
FAU - Jin, Yuanxiang
AU  - Jin Y
AD  - Department of Biotechnology, College of Biotechnology and Bioengineering, 
      Zhejiang University of Technology, Hangzhou, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Carbamates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 8HLL7N9UWO (propamocarb)
SB  - IM
MH  - Animals
MH  - Bacteria/classification/genetics
MH  - Carbamates/*toxicity
MH  - Dysbiosis/chemically induced/genetics/*physiopathology
MH  - Gastrointestinal Microbiome/*drug effects/genetics/physiology
MH  - Gene Expression/drug effects
MH  - Liver/*drug effects/metabolism/pathology
MH  - Male
MH  - Metabolic Diseases/chemically induced/genetics/*physiopathology
MH  - Metabolomics/methods
MH  - RNA, Ribosomal, 16S/genetics
MH  - Signal Transduction/drug effects/genetics
MH  - Time Factors
MH  - Toxicity Tests
MH  - Zebrafish
EDAT- 2018/12/14 06:00
MHDA- 2019/05/28 06:00
CRDT- 2018/12/14 06:00
PHST- 2018/08/22 00:00 [received]
PHST- 2018/11/03 00:00 [accepted]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/05/28 06:00 [medline]
PHST- 2018/12/14 06:00 [entrez]
AID - 5244087 [pii]
AID - 10.1093/abbs/gmy153 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2019 Jan 1;51(1):88-96. doi: 
      10.1093/abbs/gmy153.