PMID- 30544527 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200929 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 8 IP - 12 DP - 2018 Dec 7 TI - Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions. LID - 10.3390/ani8120233 [doi] LID - 233 AB - Airborne transmission of porcine reproductive and respiratory syndrome virus (PRRSV) has been known for a long time. Most experiments were performed using PRRSV-2 strains and fairly little information is available on the airborne spread of PRRSV-1. The aim of this study was to assess three different air samplers for their ability to detect PRRSV-1 under experimental and field conditions. All three devices were able to detect PRRSV-1 by quantitative reverse trascription polymerase chain reaction (qRT-PCR) under experimental conditions. However, the detection of PRRSV-1 in a PRRSV-positive farm with active virus circulation was not successful. FAU - Stein, Heiko AU - Stein H AD - Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. heiko.stein@googlemail.com. FAU - Schulz, Jochen AU - Schulz J AD - Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany. jochen.schulz@tiho-hannover.de. FAU - Morgenstern, Rebecca AU - Morgenstern R AD - Boehringer Ingelheim RCV GmbH & Co KG, 1120 Vienna, Austria. rebeccalanghoff@hotmail.com. FAU - Voglmayr, Thomas AU - Voglmayr T AD - Traunkreis Vet Clinic, 4551 Ried im Traunkreis, Austria. thomas.voglmayr@vetclinic.at. FAU - Freymuller, Georg AU - Freymuller G AD - Traunkreis Vet Clinic, 4551 Ried im Traunkreis, Austria. georg.freymueller@vetclinic.at. FAU - Sinn, Leonie AU - Sinn L AD - Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. leonie.sinn@gmx.de. FAU - Rumenapf, Till AU - Rumenapf T AD - Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. till.ruemenapf@vetmeduni.ac.at. FAU - Hennig-Pauka, Isabel AU - Hennig-Pauka I AD - Field Station for Epidemiology, University of Veterinary Medicine Hannover, Foundation, 49456 Bakum, Germany. Isabel.hennig-pauka@tiho-hannover.de. FAU - Ladinig, Andrea AU - Ladinig A AD - Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. andrea.ladinig@vetmeduni.ac.at. LA - eng PT - Journal Article DEP - 20181207 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC6315328 OTO - NOTNLM OT - PRRSV OT - aerosol OT - air sampler OT - airborne COIS- The authors declare no conflict of interest. EDAT- 2018/12/14 06:00 MHDA- 2018/12/14 06:01 PMCR- 2018/12/07 CRDT- 2018/12/15 06:00 PHST- 2018/10/05 00:00 [received] PHST- 2018/11/22 00:00 [revised] PHST- 2018/12/05 00:00 [accepted] PHST- 2018/12/15 06:00 [entrez] PHST- 2018/12/14 06:00 [pubmed] PHST- 2018/12/14 06:01 [medline] PHST- 2018/12/07 00:00 [pmc-release] AID - ani8120233 [pii] AID - animals-08-00233 [pii] AID - 10.3390/ani8120233 [doi] PST - epublish SO - Animals (Basel). 2018 Dec 7;8(12):233. doi: 10.3390/ani8120233.