PMID- 30544620
OWN - NLM
STAT- MEDLINE
DCOM- 20190218
LR  - 20231213
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 23
IP  - 12
DP  - 2018 Dec 7
TI  - Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant 
      Products Using Soybean Lipoxygenase-Based Methods. An Overview.
LID - 10.3390/molecules23123244 [doi]
LID - 3244
AB  - In the last decades, increasing demand of antioxidant-rich foods and growing 
      interest in their putative role in prevention of degenerative diseases have 
      promoted development of methods for measuring Antioxidant Capacity (AC). 
      Nevertheless, most of these assays use radicals and experimental conditions far 
      from the physiological ones, and are able to estimate only one or a few 
      antioxidant mechanisms. On the other hand, the novel LOX/RNO and LOX(-)FL methods, 
      based on secondary reactions between the soybean lipoxygenase (LOX)-1 isoenzyme 
      and either 4-nitroso-N,N-dimethylaniline (RNO) or fluorescein (FL), may provide a 
      more comprehensive AC evaluation. In fact, they are able to detect simultaneously 
      many antioxidant functions (scavenging of some physiological radical species, 
      iron ion reducing and chelating activities, inhibition of the pro-oxidant 
      apoenzyme) and to highlight synergism among phytochemicals. They are applied to 
      dissect antioxidant properties of several natural plant products: food-grade 
      antioxidants, cereal and pseudocereal grains, grain-derived products, fruits. 
      Recently, LOX(-)FL has been used for ex vivo AC measurements of human blood samples 
      after short- and long-term intakes of some of these foods, and the effectiveness 
      in improving serum antioxidant status was evaluated using the novel 
      Antioxidant/Oxidant Balance (AOB) parameter, calculated as an AC/Peroxide Level 
      ratio. An overview of data is presented.
FAU - Soccio, Mario
AU  - Soccio M
AUID- ORCID: 0000-0003-3115-200X
AD  - Department of the Sciences of Agriculture, Food and Environment, University of 
      Foggia, Via Napoli 25, 71122 Foggia, Italy. mario.soccio@unifg.it.
FAU - Laus, Maura N
AU  - Laus MN
AD  - Department of the Sciences of Agriculture, Food and Environment, University of 
      Foggia, Via Napoli 25, 71122 Foggia, Italy. maura.laus@unifg.it.
FAU - Flagella, Zina
AU  - Flagella Z
AD  - Department of the Sciences of Agriculture, Food and Environment, University of 
      Foggia, Via Napoli 25, 71122 Foggia, Italy. zina.flagella@unifg.it.
FAU - Pastore, Donato
AU  - Pastore D
AD  - Department of the Sciences of Agriculture, Food and Environment, University of 
      Foggia, Via Napoli 25, 71122 Foggia, Italy.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20181207
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Antioxidants)
RN  - 0 (Biological Products)
RN  - EC 1.13.11.- (lipoxygenase L-1)
RN  - EC 1.13.11.12 (Lipoxygenase)
SB  - IM
MH  - Antioxidants/*pharmacology
MH  - Biological Products/*pharmacology
MH  - Biotechnology/*methods
MH  - Humans
MH  - Lipoxygenase/*metabolism
MH  - Glycine max/*enzymology
PMC - PMC6320953
OTO - NOTNLM
OT  - Antioxidant/Oxidant Balance
OT  - LOX/RNO method
OT  - LOX-FL method
OT  - ORAC
OT  - TEAC
OT  - antioxidant activity
OT  - blood antioxidant status
OT  - lipoxygenase
OT  - phytochemicals
COIS- The authors declare no conflicts of interest.
EDAT- 2018/12/14 06:00
MHDA- 2019/02/20 06:00
PMCR- 2018/12/07
CRDT- 2018/12/15 06:00
PHST- 2018/11/20 00:00 [received]
PHST- 2018/12/05 00:00 [revised]
PHST- 2018/12/06 00:00 [accepted]
PHST- 2018/12/15 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/02/20 06:00 [medline]
PHST- 2018/12/07 00:00 [pmc-release]
AID - molecules23123244 [pii]
AID - molecules-23-03244 [pii]
AID - 10.3390/molecules23123244 [doi]
PST - epublish
SO  - Molecules. 2018 Dec 7;23(12):3244. doi: 10.3390/molecules23123244.