PMID- 30544702 OWN - NLM STAT- MEDLINE DCOM- 20190222 LR - 20200225 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 10 IP - 12 DP - 2018 Dec 10 TI - Bojungikgi-Tang, a Traditional Herbal Formula, Exerts Neuroprotective Effects and Ameliorates Memory Impairments in Alzheimer's Disease-Like Experimental Models. LID - 10.3390/nu10121952 [doi] LID - 1952 AB - Bojungikgi-tang (BJIGT; Bu Zhong Yi Qi Tang in China, Hochuekkito in Japan) is a traditional Oriental herbal formula comprised of eight medicinal herbs that has long been used for the treatment of digestive disorders. A recent clinical study from South Korea reported that BJIGT-gamibang administration may be effective in treating dementia. We aimed to establish scientific evidence for the anti-dementia effects of BJIGT using in vitro and in vivo experimental models. We measured amyloid- beta (Abeta) aggregation, beta-secretase (BACE), and antioxidant activity in a cell free system. Neuroprotective effects were assessed using CCK-8. Imprinting control region (ICR) mice were divided into the following six groups: Normal control, Abeta-injected, Abeta-injection + oral BJIGT gavage (200, 400, or 800 mg/kg/day), and Abeta-injection + oral morin administration (10 mg/kg/day). Subsequently, behavioral evaluations were conducted and brain samples were collected from all the animals and assessed. BJIGT enhanced inhibition of Abeta aggregation and BACE activity in vivo, as well as antioxidant activity in in vitro, cell-free systems. BJIGT also exerted neuroprotective effects in a hydroperoxide (H(2)O(2))-induced damaged HT22 hippocampal cell line model. In addition, BJIGT administration significantly ameliorated cognitive impairments in Abeta-injected mice, as assessed by the passive avoidance and Y-maze tests. Furthermore, BJIGT treatment suppressed Abeta aggregation and expression, as well as expression of Abeta, NeuN, and brain-derived neurotrophic factor (BDNF) in the hippocampi of Abeta-injected mice. Overall, our results demonstrate that, with further testing in clinical populations, BJIGT may have great utility for the treatment of dementia and especially Alzheimer's disease. FAU - Lim, Hye-Sun AU - Lim HS AUID- ORCID: 0000-0003-0727-3864 AD - Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. qp1015@kiom.re.kr. AD - Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Jeollanam-do 58245, Korea. qp1015@kiom.re.kr. FAU - Kim, Yu Jin AU - Kim YJ AUID- ORCID: 0000-0001-5152-9439 AD - Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. jinjin0228@kiom.re.kr. AD - College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. jinjin0228@kiom.re.kr. FAU - Sohn, Eunjin AU - Sohn E AD - Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. ssen4022@kiom.re.kr. FAU - Yoon, Jiyeon AU - Yoon J AD - Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. jyeon7139@kiom.re.kr. FAU - Kim, Bu-Yeo AU - Kim BY AD - Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. buykim@kiom.re.kr. FAU - Jeong, Soo-Jin AU - Jeong SJ AD - Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. sjijeong@kiom.re.kr. LA - eng PT - Journal Article DEP - 20181210 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Drugs, Chinese Herbal) RN - 0 (Neuroprotective Agents) RN - 0 (bu-zhong-yi-qi-tang) SB - IM MH - Alzheimer Disease/*metabolism MH - Animals MH - Behavior, Animal/drug effects MH - Cell Line MH - Disease Models, Animal MH - Drugs, Chinese Herbal/analysis/chemistry/*pharmacology MH - Humans MH - Male MH - Memory/*drug effects MH - Mice MH - Mice, Inbred ICR MH - Neuroprotective Agents/analysis/chemistry/*pharmacology PMC - PMC6316759 OTO - NOTNLM OT - Alzheimer's disease OT - Bojungikgi-tang OT - amyloid-beta OT - memory loss OT - neuroprotection COIS- The authors declare no conflict of interest. EDAT- 2018/12/14 06:00 MHDA- 2019/02/23 06:00 PMCR- 2018/12/01 CRDT- 2018/12/15 06:00 PHST- 2018/11/16 00:00 [received] PHST- 2018/12/07 00:00 [revised] PHST- 2018/12/07 00:00 [accepted] PHST- 2018/12/15 06:00 [entrez] PHST- 2018/12/14 06:00 [pubmed] PHST- 2019/02/23 06:00 [medline] PHST- 2018/12/01 00:00 [pmc-release] AID - nu10121952 [pii] AID - nutrients-10-01952 [pii] AID - 10.3390/nu10121952 [doi] PST - epublish SO - Nutrients. 2018 Dec 10;10(12):1952. doi: 10.3390/nu10121952.