PMID- 30544747
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200225
IS  - 2073-4409 (Print)
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 7
IP  - 12
DP  - 2018 Dec 10
TI  - Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of 
      Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction 
      Molecule 1 (STIM1) Proteins.
LID - 10.3390/cells7120262 [doi]
LID - 262
AB  - The incidence of colorectal cancer (CRC) has significantly increased in recent 
      decades, and this disease has become an important health issue worldwide. 
      Currently, there is no useful prognostic or diagnostic biomarker for CRC. Heat 
      shock protein 27 (HSP27) is a chaperone that interacts with many proteins. HSP27 
      has been shown to be overexpressed in many cancers, including colon cancer, and 
      its overexpression is related to poor disease outcome. Although the importance of 
      HSP27 as a biomarker cannot be underrated, its detailed mechanisms in colon 
      cancer are still unclear. In vitro studies have indicated that silencing HSP27 
      reduces the proliferation, migration and invasion of colon cancer cells, and 
      xenograft models have shown that silencing HSP27 decreases tumor progression. 
      Tissue array results showed that colon cancer patients with high expression of 
      HSP27 exhibited poor prognosis. In addition, we found a reduction of calcium 
      influx through a decrease in STIM1 protein after HSP27 was abolished. The 
      formation of puncta was decreased in HSP27 knockdown (HSP27KD) cells after 
      thapsigargin (TG) treatment. Finally, we confirmed that the reduction of STIM1 
      after HSP27 silencing may be due to a loss of STIM1 stability instead of 
      transcription. HSP27 may interact with STIM1 but not Orai1, as shown by 
      immunoprecipitation assays. HSP27 and STIM1 were co-expressed in CRC specimens. 
      Our study showed that HSP27 is a key mediator in the progression and metastasis 
      of CRC by regulating the store-operated calcium entry. This novel pathway may 
      provide a new direction for development of therapeutic strategies for CRC.
FAU - Huang, Chien-Yu
AU  - Huang CY
AD  - Department of Surgery, College of Medicine, Taipei Medical University, Taipei 
      110, Taiwan. cyh@tmu.edu.tw.
AD  - Division of General Surgery, Department of Surgery, Shuang Ho Hospital, Taipei 
      Medical University, Taipei 110, Taiwan. cyh@tmu.edu.tw.
FAU - Wei, Po-Li
AU  - Wei PL
AD  - Department of Surgery, College of Medicine, Taipei Medical University, Taipei 
      110, Taiwan. poliwei@tmu.edu.tw.
AD  - Division of Colorectal Surgery, Department of Surgery, Wan Fang Hospital, Taipei 
      Medical University, Taipei 110, Taiwan. poliwei@tmu.edu.tw.
AD  - Cancer Research Center and Translational Laboratory, Department of Medical 
      Research, Taipei Medical University Hospital, Taipei Medical University, Taipei 
      110, Taiwan. poliwei@tmu.edu.tw.
AD  - Division of Colorectal Surgery, Department of Surgery, Taipei Medical University 
      Hospital, Taipei Medical University, Taipei 110, Taiwan. poliwei@tmu.edu.tw.
AD  - Graduate Institute of Cancer Biology and Drug Discovery, Taipei Medical 
      University, Taipei 110, Taiwan. poliwei@tmu.edu.tw.
FAU - Chen, Wei-Yu
AU  - Chen WY
AD  - Department of Pathology, School of Medicine, College of Medicine, Taipei Medical 
      University, Taipei 110, Taiwan. wychen.patho@gmail.com.
AD  - Department of Pathology, Wan Fang Hospital, Taipei Medical University, Taipei 
      110, Taiwan. wychen.patho@gmail.com.
FAU - Chang, Wei-Chiao
AU  - Chang WC
AD  - School of Pharmacy, Taipei Medical University, Taipei 110, Taiwan. 
      weichiao.chang@gmail.com.
AD  - Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, Taipei 
      Medical University, Taipei 110, Taiwan. weichiao.chang@gmail.com.
FAU - Chang, Yu-Jia
AU  - Chang YJ
AUID- ORCID: 0000-0003-3978-3244
AD  - Cancer Research Center and Translational Laboratory, Department of Medical 
      Research, Taipei Medical University Hospital, Taipei Medical University, Taipei 
      110, Taiwan. r5424012@tmu.edu.tw.
AD  - Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical 
      University, Taipei 110, Taiwan. r5424012@tmu.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20181210
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
PMC - PMC6315635
OTO - NOTNLM
OT  - CRC
OT  - ER
OT  - HSP27
OT  - SOCE
OT  - STIM1
COIS- The authors have no conflict of interest in this study.
EDAT- 2018/12/14 06:00
MHDA- 2018/12/14 06:01
PMCR- 2018/12/01
CRDT- 2018/12/15 06:00
PHST- 2018/11/13 00:00 [received]
PHST- 2018/12/02 00:00 [revised]
PHST- 2018/12/07 00:00 [accepted]
PHST- 2018/12/15 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2018/12/14 06:01 [medline]
PHST- 2018/12/01 00:00 [pmc-release]
AID - cells7120262 [pii]
AID - cells-07-00262 [pii]
AID - 10.3390/cells7120262 [doi]
PST - epublish
SO  - Cells. 2018 Dec 10;7(12):262. doi: 10.3390/cells7120262.