PMID- 30546011
OWN - NLM
STAT- MEDLINE
DCOM- 20190122
LR  - 20200309
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 9
IP  - 1
DP  - 2018 Dec 13
TI  - Tissue engineering of human hair follicles using a biomimetic developmental 
      approach.
PG  - 5301
LID - 10.1038/s41467-018-07579-y [doi]
LID - 5301
AB  - Human skin constructs (HSCs) have the potential to provide an effective therapy 
      for patients with significant skin injuries and to enable human-relevant drug 
      screening for skin diseases; however, the incorporation of engineered skin 
      appendages, such as hair follicles (HFs), into HSCs remains a major challenge. 
      Here, we demonstrate a biomimetic approach for generation of human HFs within 
      HSCs by recapitulating the physiological 3D organization of cells in the HF 
      microenvironment using 3D-printed molds. Overexpression of Lef-1 in dermal 
      papilla cells (DPC) restores the intact DPC transcriptional signature and 
      significantly enhances the efficiency of HF differentiation in HSCs. Furthermore, 
      vascularization of hair-bearing HSCs prior to engraftment allows for efficient 
      human hair growth in immunodeficient mice. The ability to regenerate an entire HF 
      from cultured human cells will have a transformative impact on the medical 
      management of different types of alopecia, as well as chronic wounds, which 
      represent major unmet medical needs.
FAU - Abaci, Hasan Erbil
AU  - Abaci HE
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Coffman, Abigail
AU  - Coffman A
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Doucet, Yanne
AU  - Doucet Y
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Chen, James
AU  - Chen J
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA.
AD  - Department of Systems Biology, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Jackow, Joanna
AU  - Jackow J
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Wang, Etienne
AU  - Wang E
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Guo, Zongyou
AU  - Guo Z
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Shin, Jung U
AU  - Shin JU
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Jahoda, Colin A
AU  - Jahoda CA
AD  - Department of Biosciences, Durham University, Durham, UK.
FAU - Christiano, Angela M
AU  - Christiano AM
AD  - Department of Dermatology, Columbia University Medical Center, New York, NY, 
      10032, USA. amc65@cumc.columbia.edu.
AD  - Department of Genetics and Development, Columbia University Medical Center, New 
      York, NY, 10032, USA. amc65@cumc.columbia.edu.
LA  - eng
GR  - K01 AR072131/AR/NIAMS NIH HHS/United States
GR  - P30 AR069632/AR/NIAMS NIH HHS/United States
GR  - UH2 EB017103/EB/NIBIB NIH HHS/United States
GR  - UL1 TR001873/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20181213
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (LEF1 protein, human)
RN  - 0 (Lymphoid Enhancer-Binding Factor 1)
SB  - IM
MH  - Alopecia/pathology/*therapy
MH  - Animals
MH  - Biomimetics
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dermis/*cytology
MH  - Hair Follicle/cytology/*growth & development/*transplantation
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Lymphoid Enhancer-Binding Factor 1/biosynthesis
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Tissue Engineering/*methods
MH  - Transplantation, Heterologous
PMC - PMC6294003
COIS- A.M.C. and C.A.J. are founders of Rapunzel Bioscience Inc, focused on developing 
      regenerative therapies for skin and hair disorders. The remaining authors declare 
      no competing interests.
EDAT- 2018/12/14 06:00
MHDA- 2019/01/23 06:00
PMCR- 2018/12/13
CRDT- 2018/12/15 06:00
PHST- 2018/05/29 00:00 [received]
PHST- 2018/10/25 00:00 [accepted]
PHST- 2018/12/15 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/01/23 06:00 [medline]
PHST- 2018/12/13 00:00 [pmc-release]
AID - 10.1038/s41467-018-07579-y [pii]
AID - 7579 [pii]
AID - 10.1038/s41467-018-07579-y [doi]
PST - epublish
SO  - Nat Commun. 2018 Dec 13;9(1):5301. doi: 10.1038/s41467-018-07579-y.