PMID- 30546025
OWN - NLM
STAT- MEDLINE
DCOM- 20191021
LR  - 20200309
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Dec 13
TI  - MicroRNAs derived from the insect virus HzNV-1 promote lytic infection by 
      suppressing histone methylation.
PG  - 17817
LID - 10.1038/s41598-018-35782-w [doi]
LID - 17817
AB  - Heliothis zea nudivirus-1 (HzNV-1) is an insect virus that can induce both lytic 
      and latent infections in various insect cell lines. During latent infection, 
      several microRNAs (miRNAs) are produced from persistency-associated gene 1 (pag1) 
      as the only detectable HzNV-1 transcript. Previous studies have shown that the 
      pag1 gene suppresses the immediate-early gene hhi1 and promotes host switching 
      into a latent infection via miRNAs derived from pag1. Although other functions of 
      the miRNAs derived from pag1 have not yet been elucidated, several studies have 
      suggested that miRNAs encoded from latency-associated genes can regulate 
      histone-associated enzymes. Because pag1 is a noncoding transcript, it 
      potentially regulates host chromatin structure through miRNAs upon infection. 
      Nevertheless, the exact mechanism by which pag1 alters viral infections remains 
      unknown. In this study, we found that the pag1-encoded miRNA miR-420 suppresses 
      expression of the histone modification-associated enzyme su(var)3-9. Therefore, 
      this miRNA causes histone modification to promote HzNV-1 infection. These results 
      suggest that HzNV-1 may directly influence epigenetic regulation in host cells 
      through interactions with pag1 miRNAs to promote lytic infection. This study 
      provides us with a better understanding of both the HzNV-1 infection pathway and 
      the relationship between viral miRNAs and epigenetic regulation.
FAU - Wu, Pei-Chi
AU  - Wu PC
AD  - Department of Entomology, National Taiwan University, Taipei, 106, Taiwan.
FAU - Lin, Yu-Hsien
AU  - Lin YH
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
FAU - Wu, Tsai-Chin
AU  - Wu TC
AD  - Department of Entomology, National Taiwan University, Taipei, 106, Taiwan.
FAU - Lee, Song-Tay
AU  - Lee ST
AD  - Department of Biotechnology, Southern Taiwan University of Technology, Tainan, 
      710, Taiwan.
FAU - Wu, Carol-P
AU  - Wu CP
AD  - Department of Entomology, National Taiwan University, Taipei, 106, Taiwan.
FAU - Chang, Yuan
AU  - Chang Y
AD  - Department of Entomology, National Taiwan University, Taipei, 106, Taiwan.
FAU - Wu, Yueh-Lung
AU  - Wu YL
AD  - Department of Entomology, National Taiwan University, Taipei, 106, Taiwan. 
      runwu@ntu.edu.tw.
LA  - eng
GR  - 107-2311-B-002-024-MY3/Ministry of Science and Technology, Taiwan (Ministry of 
      Science and Technology of Taiwan)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181213
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Histones)
RN  - 0 (Insect Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Pag1 protein, Heliothis zea virus 1)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
RN  - Helicoverpa armigera nucleopolyhedrovirus
SB  - IM
MH  - Animals
MH  - *Epigenesis, Genetic
MH  - *Gene Expression Regulation, Viral
MH  - Histones/*metabolism
MH  - Insect Proteins/*metabolism
MH  - Methylation
MH  - MicroRNAs/*biosynthesis
MH  - Nucleopolyhedroviruses/*physiology
MH  - RNA, Viral/*biosynthesis
MH  - Sf9 Cells
MH  - *Spodoptera/metabolism/virology
MH  - Viral Proteins/metabolism
PMC - PMC6292938
COIS- The authors declare no competing interests.
EDAT- 2018/12/14 06:00
MHDA- 2019/10/23 06:00
PMCR- 2018/12/13
CRDT- 2018/12/15 06:00
PHST- 2018/08/02 00:00 [received]
PHST- 2018/11/06 00:00 [accepted]
PHST- 2018/12/15 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/10/23 06:00 [medline]
PHST- 2018/12/13 00:00 [pmc-release]
AID - 10.1038/s41598-018-35782-w [pii]
AID - 35782 [pii]
AID - 10.1038/s41598-018-35782-w [doi]
PST - epublish
SO  - Sci Rep. 2018 Dec 13;8(1):17817. doi: 10.1038/s41598-018-35782-w.