PMID- 30546350
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 9
DP  - 2018
TI  - Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With H(2)S 
      Production: Escherichia coli K-12 and Desulfovibrio sp. Strain DF1 as Co-culture 
      Model.
PG  - 2792
LID - 10.3389/fmicb.2018.02792 [doi]
LID - 2792
AB  - Sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) is produced by plants and other 
      phototrophs and its biodegradation is a relevant component of the biogeochemical 
      carbon and sulfur cycles. SQ is known to be degraded by aerobic bacterial 
      consortia in two tiers via C(3)-organosulfonates as transient intermediates to 
      CO(2), water and sulfate. In this study, we present a first laboratory model for 
      anaerobic degradation of SQ by bacterial consortia in two tiers to acetate and 
      hydrogen sulfide (H(2)S). For the first tier, SQ-degrading Escherichia coli K-12 
      was used. It catalyzes the fermentation of SQ to 2,3-dihydroxypropane-1-sulfonate 
      (DHPS), succinate, acetate and formate, thus, a novel type of mixed-acid 
      fermentation. It employs the characterized SQ Embden-Meyerhof-Parnas pathway, as 
      confirmed by mutational and proteomic analyses. For the second tier, a 
      DHPS-degrading Desulfovibrio sp. isolate from anaerobic sewage sludge was used, 
      strain DF1. It catalyzes another novel fermentation, of the DHPS to acetate and 
      H(2)S. Its DHPS desulfonation pathway was identified by differential proteomics 
      and demonstrated by heterologously produced enzymes: DHPS is oxidized via 
      3-sulfolactaldehyde to 3-sulfolactate (SL) by two NAD(+)-dependent dehydrogenases 
      (DhpA, SlaB); the SL is cleaved by an SL sulfite-lyase known from aerobic 
      bacteria (SuyAB) to pyruvate and sulfite. The pyruvate is oxidized to acetate, 
      while the sulfite is used as electron acceptor in respiration and reduced to 
      H(2)S. In conclusion, anaerobic sulfidogenic SQ degradation was demonstrated as a 
      novel link in the biogeochemical sulfur cycle. SQ is also a constituent of the 
      green-vegetable diet of herbivores and omnivores and H(2)S production in the 
      intestinal microbiome has many recognized and potential contributions to human 
      health and disease. Hence, it is important to examine bacterial SQ degradation 
      also in the human intestinal microbiome, in relation to H(2)S production, dietary 
      conditions and human health.
FAU - Burrichter, Anna
AU  - Burrichter A
AD  - Department of Biology, University of Konstanz, Konstanz, Germany.
AD  - The Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, 
      Germany.
FAU - Denger, Karin
AU  - Denger K
AD  - Department of Biology, University of Konstanz, Konstanz, Germany.
FAU - Franchini, Paolo
AU  - Franchini P
AD  - Department of Biology, University of Konstanz, Konstanz, Germany.
FAU - Huhn, Thomas
AU  - Huhn T
AD  - The Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, 
      Germany.
AD  - Department of Chemistry, University of Konstanz, Konstanz, Germany.
FAU - Muller, Nicolai
AU  - Muller N
AD  - Department of Biology, University of Konstanz, Konstanz, Germany.
FAU - Spiteller, Dieter
AU  - Spiteller D
AD  - Department of Biology, University of Konstanz, Konstanz, Germany.
AD  - The Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, 
      Germany.
FAU - Schleheck, David
AU  - Schleheck D
AD  - Department of Biology, University of Konstanz, Konstanz, Germany.
AD  - The Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20181127
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6278857
OTO - NOTNLM
OT  - anaerobic bacterial metabolism
OT  - biogeochemical carbon and sulfur cycle
OT  - gut microbiome
OT  - human health and disease
OT  - organosulfonate respiration
OT  - plant sulfolipid
OT  - sulfidogenesis
OT  - sulfoquinovosyldiacylglycerol
EDAT- 2018/12/14 06:00
MHDA- 2018/12/14 06:01
PMCR- 2018/11/27
CRDT- 2018/12/15 06:00
PHST- 2018/08/24 00:00 [received]
PHST- 2018/10/30 00:00 [accepted]
PHST- 2018/12/15 06:00 [entrez]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2018/12/14 06:01 [medline]
PHST- 2018/11/27 00:00 [pmc-release]
AID - 10.3389/fmicb.2018.02792 [doi]
PST - epublish
SO  - Front Microbiol. 2018 Nov 27;9:2792. doi: 10.3389/fmicb.2018.02792. eCollection 
      2018.