PMID- 30547194
OWN - NLM
STAT- MEDLINE
DCOM- 20190718
LR  - 20200225
IS  - 1432-184X (Electronic)
IS  - 0095-3628 (Linking)
VI  - 78
IP  - 2
DP  - 2019 Aug
TI  - Dynamics of Biofilm Formation by Salmonella Typhimurium and Beef Processing Plant 
      Bacteria in Mono- and Dual-Species Cultures.
PG  - 375-387
LID - 10.1007/s00248-018-1304-z [doi]
AB  - This study aimed to determine the impact of bacteria from a beef plant conveyor 
      belt on the biofilm formation of Salmonella in dual-species cultures. Beef plant 
      isolates (50) including 18 Gram-negative aerobes (GNA), 8 Gram-positive aerobes 
      (GPA), 5 lactic acid bacteria (LAB), 9 Enterobacteriaceae (EB), and 10 generic 
      Escherichia coli (GEC) were included for developing biofilms in mono- and 
      co-culture with S. Typhimurium at 15  degrees C for 6 days. Five selected cultures in 
      planktonic form and in biofilms were tested for susceptibility to two commonly 
      used sanitizers (i.e. E-San and Perox-E Plus). In mono-cultures, >/= 80, 67, 61, 
      20, and 13% of GEC, EB, GNA, LAB, and GPA, respectively, developed measurable 
      biofilms after 2 days, while all co-culture pairings with S. Typhimurium achieved 
      some level of biofilm production. The predominant effect of EB and only effect of 
      GEC strains on the biofilm formation of S. Typhimurium was antagonistic, while 
      that of Gram-positive bacteria was synergistic, with the effect being more 
      prominent on day 6. The effect was highly variable for the GNA isolates. Six 
      aerobic isolates that formed moderate/strong biofilms by day 2 greatly boosted 
      the co-culture biofilm formation. Seven Gram-negative bacteria were antagonistic 
      against the biofilm formation of the co-cultures. Both sanitizers completely 
      inactivated the selected planktonic cultures, but were largely ineffective 
      against biofilms. In conclusion, all beef plant isolates assessed formed biofilms 
      when paired with S. Typhimurium. Aerobic biofilm formers may create a more 
      favorable condition for Salmonella biofilm formation, while some beef plant 
      isolates have potential as a biocontrol strategy for Salmonella biofilms.
FAU - Visvalingam, Jeyachchandran
AU  - Visvalingam J
AD  - Agriculture and Agri-Food Canada Lacombe Research and Development Centre, 6000 C 
      & E Trail, Lacombe, Alberta, T4L 1W1, Canada.
AD  - Kane Biotech Inc, 196 Innovation Drive, Winnipeg, Manitoba, R3T 2N2, Canada.
FAU - Zhang, Peipei
AU  - Zhang P
AD  - Agriculture and Agri-Food Canada Lacombe Research and Development Centre, 6000 C 
      & E Trail, Lacombe, Alberta, T4L 1W1, Canada.
FAU - Ells, Timothy C
AU  - Ells TC
AD  - Agriculture and Agri-Food Canada Kentville Research and Development Centre, 32 
      Main Street, Kentville, Nova Scotia, B4N 1J5, Canada.
FAU - Yang, Xianqin
AU  - Yang X
AD  - Agriculture and Agri-Food Canada Lacombe Research and Development Centre, 6000 C 
      & E Trail, Lacombe, Alberta, T4L 1W1, Canada. xianqin.yang@canada.ca.
LA  - eng
GR  - A-1603 and A-1637/Agriculture and Agri-Food Canada/
PT  - Journal Article
DEP - 20181213
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
SB  - IM
MH  - Animals
MH  - Bacteria/classification/genetics/*isolation & purification
MH  - Bacterial Physiological Phenomena
MH  - Biodiversity
MH  - *Biofilms
MH  - Cattle
MH  - Food Handling
MH  - Red Meat/analysis/*microbiology
MH  - Salmonella typhimurium/genetics/isolation & purification/*physiology
OTO - NOTNLM
OT  - Antagonism
OT  - Biofilm
OT  - Environmental microbiota
OT  - Interaction
OT  - Sanitization
EDAT- 2018/12/14 06:00
MHDA- 2019/07/19 06:00
CRDT- 2018/12/15 06:00
PHST- 2018/06/20 00:00 [received]
PHST- 2018/11/29 00:00 [accepted]
PHST- 2018/12/14 06:00 [pubmed]
PHST- 2019/07/19 06:00 [medline]
PHST- 2018/12/15 06:00 [entrez]
AID - 10.1007/s00248-018-1304-z [pii]
AID - 10.1007/s00248-018-1304-z [doi]
PST - ppublish
SO  - Microb Ecol. 2019 Aug;78(2):375-387. doi: 10.1007/s00248-018-1304-z. Epub 2018 
      Dec 13.