PMID- 30548426
OWN - NLM
STAT- MEDLINE
DCOM- 20190917
LR  - 20231004
IS  - 2051-817X (Electronic)
IS  - 2051-817X (Linking)
VI  - 6
IP  - 23
DP  - 2018 Dec
TI  - PI3K-AKT-FOXO1 pathway targeted by skeletal muscle microRNA to suppress 
      proteolytic gene expression in response to carbohydrate intake during aerobic 
      exercise.
PG  - e13931
LID - 10.14814/phy2.13931 [doi]
LID - e13931
AB  - Ingesting protein and carbohydrate together during aerobic exercise suppresses 
      the expression of specific skeletal muscle microRNA and promotes muscle 
      hypertrophy. Determining whether there are independent effects of carbohydrate 
      and protein on microRNA will allow for a clearer understanding of the mechanistic 
      role microRNA serve in regulating skeletal muscle protein synthetic and 
      proteolytic responses to nutrition and exercise. This study determined skeletal 
      muscle microRNA responses to aerobic exercise with or without carbohydrate, and 
      recovery whey protein (WP). Seventeen males were randomized to consume 
      carbohydrate (CHO; 145 g; n = 9) or non-nutritive control (CON; n = 8) beverages 
      during exercise. Muscle was collected before (BASE) and after 80 min of 
      steady-state exercise (1.7 +/- 0.3 V̇O(2)  L.min(-1) ) followed by a 2-mile time 
      trial (17.9 +/- 3.5 min; POST), and 3-h into recovery after consuming WP (25 g; 
      REC). RT-qPCR was used to determine microRNA and mRNA expression. Bioinformatics 
      analysis was conducted using the mirPath software. Western blotting was used to 
      assess protein signaling. The expression of six microRNA (miR-19b-3p, miR-99a-5p, 
      miR-100-5p, miR-222-3p, miR-324-3p, and miR-486-5p) were higher (P < 0.05) in CHO 
      compared to CON, all of which target the PI3K-AKT, ubiquitin proteasome, FOXO, 
      and mTORC1 pathways. p-AKT(T)(hr473) and p-FOXO1(Thr24) were higher (P < 0.05) in 
      POST CHO compared to CON. The expression of PTEN was lower (P < 0.05) in REC CHO 
      than CON, while MURF1 was lower (P < 0.05) POST CHO than CON. These findings 
      suggest the mechanism by which microRNA facilitate skeletal muscle adaptations in 
      response to exercise with carbohydrate and protein feeding is by inhibiting 
      markers of proteolysis.
CI  - (c) 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on 
      behalf of The Physiological Society and the American Physiological Society.
FAU - Margolis, Lee M
AU  - Margolis LM
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
AD  - Oak Ridge Institute of Science and Education, Oak Ridge, Tennessee.
FAU - Berryman, Claire E
AU  - Berryman CE
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
AD  - Oak Ridge Institute of Science and Education, Oak Ridge, Tennessee.
AD  - Department of Nutrition, Food, and Exercise Sciences, Florida State University, 
      Tallahassee, Florida.
FAU - Murphy, Nancy E
AU  - Murphy NE
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - Carrigan, Christopher T
AU  - Carrigan CT
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - Young, Andrew J
AU  - Young AJ
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
AD  - Oak Ridge Institute of Science and Education, Oak Ridge, Tennessee.
FAU - Carbone, John W
AU  - Carbone JW
AD  - Oak Ridge Institute of Science and Education, Oak Ridge, Tennessee.
AD  - School of Health Sciences, Eastern Michigan University, Ypsilanti, Michigan.
FAU - Pasiakos, Stefan M
AU  - Pasiakos SM
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - United States
TA  - Physiol Rep
JT  - Physiological reports
JID - 101607800
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Proteins)
RN  - 0 (FOXO1 protein, human)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (MicroRNAs)
RN  - 0 (Muscle Proteins)
RN  - 0 (Tripartite Motif Proteins)
RN  - EC 2.3.2.27 (TRIM63 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Dietary Carbohydrates/administration & dosage/*pharmacology
MH  - Dietary Proteins/administration & dosage/pharmacology
MH  - Forkhead Box Protein O1/genetics/metabolism
MH  - Humans
MH  - Male
MH  - MicroRNAs/genetics/*metabolism
MH  - Muscle Proteins/genetics/metabolism
MH  - Muscle, Skeletal/drug effects/*metabolism/physiology
MH  - PTEN Phosphohydrolase/genetics/metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Physical Conditioning, Human/*methods
MH  - *Proteolysis
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - *Signal Transduction
MH  - Tripartite Motif Proteins/genetics/metabolism
MH  - Ubiquitin-Protein Ligases/genetics/metabolism
MH  - Young Adult
PMC - PMC6289907
OTO - NOTNLM
OT  - miR-206
OT  - miR-486
OT  - muscle protein breakdown
OT  - muscle protein synthesis
OT  - myomiR
OT  - whey protein
EDAT- 2018/12/15 06:00
MHDA- 2019/09/19 06:00
PMCR- 2018/12/11
CRDT- 2018/12/15 06:00
PHST- 2018/11/05 00:00 [received]
PHST- 2018/11/06 00:00 [revised]
PHST- 2018/12/15 06:00 [entrez]
PHST- 2018/12/15 06:00 [pubmed]
PHST- 2019/09/19 06:00 [medline]
PHST- 2018/12/11 00:00 [pmc-release]
AID - PHY213931 [pii]
AID - 10.14814/phy2.13931 [doi]
PST - ppublish
SO  - Physiol Rep. 2018 Dec;6(23):e13931. doi: 10.14814/phy2.13931.