PMID- 30550991 OWN - NLM STAT- MEDLINE DCOM- 20190523 LR - 20190523 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 86 DP - 2019 Mar TI - Effects of inosine 5'-monophosphate supplementation in high fishmeal and high soybean diets on growth, immune-related gene expression in gibel carp (Carassius auratus gibelio var. CAS Ⅲ), and its challenge against Aeromonas hydrophila infection. PG - 913-921 LID - S1050-4648(18)30815-5 [pii] LID - 10.1016/j.fsi.2018.12.016 [doi] AB - The present study was conducted to evaluate dietary inosine 5'-monophosphate (5'-IMP) on growth, immune genes expression and disease resistance against Aeromonas hydrophila in juvenile gibel carp (Carassius auratus gibelio var. CAS Ⅲ) (initial body weight: 7.48 g). Six diets were formulated containing exogenous 5'-IMP at three gradient levels (0, 0.1% and 0.2%) in the high dietary fishmeal group (15% fishmeal: D1, D2, D3) and in the high dietary soybean meal group (33% soybean meal: D4, D5, D6). Each diet was randomly allotted to triplicate tanks in a recirculating system. After the feeding trial, fish were exposed to Aeromonas hydrophila challenge. Hematological and immunological responses were analyzed before and after challenge. The results indicated that feeding rate in all 5'-IMP supplemented treatments (D2, D3, D5 and D6) and daily growth coefficient in D5 and D6 were reduced compared with those of respective control treatments (D1 and D4) without 5'-IMP addition (P < 0.05). The cumulative survival rates were numerically improved by dietary 5'-IMP supplementation (P > 0.05). Compared with the respective control treatment, in the high fishmeal group, plasma SOD and MPO were significantly elevated in D3 at the end of feeding trial (P < 0.05), plasma SOD and lysozyme were significantly increased in D3 after bacterial challenge (P < 0.05); in high soybean meal group, plasma lysozyme activity was significantly elevated in D5 post bacterial challenge (P < 0.05). Most of the expression of immune related genes (intelectin, major histocompatibility complex class II beta (MHC II beta), Complement 3 (C3), Complement component C7-1 (ccC7), lysozyme C, Interleukin 1beta (IL-1beta), Tumor necrosis factor alpha1 (TNF-alpha1), Transforming growth factor-beta (TGF-beta) and Interleukin 8 (IL-8)) in spleen, kidney and liver of the fish were significantly affected by supplementation of 5'-IMP at the end of feeding trial and post bacterial challenge. Additionally, adding 5'-IMP in high soybean meal diets exerted further effects of promoting immunity than counterparts in high fishmeal diets. Considering enhanced disease resistance, the immunopotentiation of 5'-IMP was manifested when the addition level was 0.1% in high soybean meal diets and 0.2% in high fishmeal diets. CI - Copyright (c) 2018 Elsevier Ltd. All rights reserved. FAU - Zhang, Peiyu AU - Zhang P AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China; University of Chinese Academy of Sciences, Beijing, PR China. FAU - Fu, Lele AU - Fu L AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China; University of Chinese Academy of Sciences, Beijing, PR China. FAU - Liu, Haokun AU - Liu H AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China. Electronic address: liuhaokun@ihb.ac.cn. FAU - Huda, Noor-Ul AU - Huda NU AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China; University of Chinese Academy of Sciences, Beijing, PR China. FAU - Zhu, Xiaoming AU - Zhu X AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China. FAU - Han, Dong AU - Han D AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, PR China. FAU - Jin, Junyan AU - Jin J AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China. FAU - Yang, Yunxia AU - Yang Y AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China. FAU - Kim, Yang-Su AU - Kim YS AD - CJ Cheiljedang, BIO Technical Marketing Team, CJ Cheiljedang Center, Seoul, 04560, South Korea. FAU - Xie, Shouqi AU - Xie S AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China. LA - eng PT - Journal Article DEP - 20181211 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Fish Proteins) RN - 131-99-7 (Inosine Monophosphate) SB - IM MH - Aeromonas hydrophila/physiology MH - Animal Feed/analysis MH - Animals MH - Diet/classification/*veterinary MH - Dietary Supplements/analysis MH - Disease Resistance/drug effects MH - Fish Diseases/immunology MH - Fish Proteins/genetics/immunology MH - Gene Expression Regulation/*immunology MH - Goldfish/*genetics/growth & development/*immunology MH - Gram-Negative Bacterial Infections/immunology/veterinary MH - Inosine Monophosphate/administration & dosage/*metabolism OTO - NOTNLM OT - 5'-IMP OT - Aeromonas hydrophila OT - Carassius auratus gibelio OT - Disease resistance OT - Immunity EDAT- 2018/12/15 06:00 MHDA- 2019/05/24 06:00 CRDT- 2018/12/15 06:00 PHST- 2018/09/13 00:00 [received] PHST- 2018/11/07 00:00 [revised] PHST- 2018/12/10 00:00 [accepted] PHST- 2018/12/15 06:00 [pubmed] PHST- 2019/05/24 06:00 [medline] PHST- 2018/12/15 06:00 [entrez] AID - S1050-4648(18)30815-5 [pii] AID - 10.1016/j.fsi.2018.12.016 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2019 Mar;86:913-921. doi: 10.1016/j.fsi.2018.12.016. Epub 2018 Dec 11.