PMID- 30553600
OWN - NLM
STAT- MEDLINE
DCOM- 20190621
LR  - 20210208
IS  - 1532-818X (Electronic)
IS  - 0196-0709 (Print)
IS  - 0196-0709 (Linking)
VI  - 40
IP  - 2
DP  - 2019 Mar-Apr
TI  - Molecular and immunologic analysis of laryngeal squamous cell carcinoma in 
      smokers and non-smokers.
PG  - 213-217
LID - S0196-0709(18)30882-2 [pii]
LID - 10.1016/j.amjoto.2018.11.009 [doi]
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is strongly associated with 
      tobacco use, but recent reports suggest an increasing incidence of LSCC in 
      patients without traditional risk factors, suggesting an alternative etiology of 
      tumorigenesis. The purpose of this study is to characterize this non-smoking 
      population and to compare immunohistochemical markers in tumor specimens from 
      non-smokers and smokers with LSCC. METHODS: A retrospective chart review of 
      patients with LSCC at Johns Hopkins Hospital (JHH) was performed. A tissue 
      microarray (TMA) was constructed with tumor specimen from non-smokers with stage 
      and age-matched smokers and stained for a variety of immunologic and molecular 
      targets. RESULTS: In the JHH cohort of 521 patients, 12% (n = 63) were 
      non-smokers. Non-smokers were more likely to be <45 years old at time of 
      diagnosis (OR 4.13, p = 0.001) and to have glottic tumors (OR 2.46, p = 0.003). 
      The TMA was comprised of tumors from 34 patients (14 non-smokers, 20 smokers). 
      Only 2 patients (6%) were human-papillomavirus (HPV) positive by high-risk RNA in 
      situ hybridization (ISH). There was no correlation between smoking status and p16 
      (p = 0.36), HPV-ISH positivity (p = 0.79), phosphatase and tensin homolog (PTEN, 
      p = 0.91), p53 (p = 0.14), or programmed death-ligand 1 (PD-L1, p = 0.27) 
      expression. CONCLUSIONS: Non-smokers with LSCC are more likely to be younger at 
      the time of diagnosis and have glottic tumors than smokers with LSCC. In TMA 
      analysis of stage and age-matched specimens from smoker and non-smokers with 
      LSCC, the pattern of expression for common molecular and immunologic markers is 
      similar. Further, HPV does not appear to be a major causative etiology of LSCC in 
      either smokers or non-smokers in our cohort of patients.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Malm, Ian-James
AU  - Malm IJ
AD  - Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins Hospital, 
      Baltimore, MD, USA. Electronic address: imalm1@jhmi.edu.
FAU - Rooper, Lisa M
AU  - Rooper LM
AD  - Department of Pathology, Johns Hopkins Hospital, Baltimore, MD, USA.
FAU - Bishop, Justin A
AU  - Bishop JA
AD  - Department of Pathology, Johns Hopkins Hospital, Baltimore, MD, USA.
FAU - Ozgursoy, Selmin Karatayli
AU  - Ozgursoy SK
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Ufuk University, Ankara, 
      Turkey.
FAU - Hillel, Alexander T
AU  - Hillel AT
AD  - Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins Hospital, 
      Baltimore, MD, USA.
FAU - Akst, Lee M
AU  - Akst LM
AD  - Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins Hospital, 
      Baltimore, MD, USA.
FAU - Best, Simon R
AU  - Best SR
AD  - Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins Hospital, 
      Baltimore, MD, USA.
LA  - eng
GR  - K23 DC014758/DC/NIDCD NIH HHS/United States
GR  - L40 DC013350/DC/NIDCD NIH HHS/United States
PT  - Journal Article
DEP - 20181122
PL  - United States
TA  - Am J Otolaryngol
JT  - American journal of otolaryngology
JID - 8000029
RN  - 0 (Antigens, CD)
RN  - 0 (B7-H1 Antigen)
RN  - 0 (CD274 protein, human)
RN  - 0 (CDKN2A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (PDCD1 protein, human)
RN  - 0 (Programmed Cell Death 1 Receptor)
RN  - 0 (RNA, Viral)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Age Factors
MH  - Aged
MH  - Antigens, CD
MH  - B7-H1 Antigen
MH  - Carcinoma, Squamous Cell/etiology/*genetics/*immunology/pathology
MH  - Cohort Studies
MH  - Cyclin-Dependent Kinase Inhibitor p16
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/etiology/*genetics/*immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - PTEN Phosphohydrolase
MH  - Papillomaviridae/genetics
MH  - Programmed Cell Death 1 Receptor
MH  - RNA, Viral
MH  - Retrospective Studies
MH  - *Smokers
MH  - Smoking/*adverse effects
PMC - PMC6485418
MID - NIHMS1516576
OTO - NOTNLM
OT  - HPV
OT  - Immunohistochemistry
OT  - Laryngeal cancer
OT  - Squamous cell carcinoma
COIS- No conflict of interest to disclose
EDAT- 2018/12/17 06:00
MHDA- 2019/06/22 06:00
PMCR- 2020/03/01
CRDT- 2018/12/17 06:00
PHST- 2018/10/22 00:00 [received]
PHST- 2018/11/05 00:00 [revised]
PHST- 2018/11/20 00:00 [accepted]
PHST- 2018/12/17 06:00 [pubmed]
PHST- 2019/06/22 06:00 [medline]
PHST- 2018/12/17 06:00 [entrez]
PHST- 2020/03/01 00:00 [pmc-release]
AID - S0196-0709(18)30882-2 [pii]
AID - 10.1016/j.amjoto.2018.11.009 [doi]
PST - ppublish
SO  - Am J Otolaryngol. 2019 Mar-Apr;40(2):213-217. doi: 10.1016/j.amjoto.2018.11.009. 
      Epub 2018 Nov 22.