PMID- 30559101
OWN - NLM
STAT- MEDLINE
DCOM- 20190523
LR  - 20190523
IS  - 0253-9772 (Print)
IS  - 0253-9772 (Linking)
VI  - 40
IP  - 12
DP  - 2018 Dec 20
TI  - [Culturing of human umbilical cord mesenchymal stem cells via a 3D suspension 
      culture system using the microcarrier].
PG  - 1120-1128
LID - 10.16288/j.yczz.18-123 [doi]
AB  - With the rapid development of stem cell research, the demands for high quality 
      stem cells in cell differentiation studies, cell therapy and clinical 
      applications increase significantly. Human umbilical cord mesenchymal stem cells 
      (hUCMSCs) hold great potentials in these applications due to its wide 
      availability, self-renewal capacity, good pluripotency, and in particular, 
      self-immunomodulation ability. However, the conventional adherent culture system 
      remains challenging in mass production of high-quality stem cells. In this study, 
      we set up a 3D suspension culture system by using spinner flasks added with 
      microcarrier. By optimizing the seeding density and rotation speed, we achieved a 
      cell density as higher as 7x10 (8)cells/L per vessel. The cultured MSCs had good 
      viability, and the expression levels of the MSC markers were similar to those of 
      2D-cultured MSCs. After being transferred back into a 2D culture system, the MSCs 
      still maintained normal morphology and proliferation ability. These results 
      indicated that the 3D suspension culture system established in this study 
      provides a fundamental basis for mass production of high-quality MSCs for future 
      research and clinical applications.
FAU - Li, Xia
AU  - Li X
AD  - College of Life Science, Northeast Agricultural University, Harbin, 150030, 
      China.
AD  - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing 100190, China.
AD  - Institute of Zoology, Chinese Academy of Science, Beijing 100101, China.
FAU - Hua, Hui Juan
AU  - Hua HJ
AD  - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing 100190, China.
AD  - Institute of Zoology, Chinese Academy of Science, Beijing 100101, China.
FAU - Hao, Jie
AU  - Hao J
AD  - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing 100190, China.
AD  - Institute of Zoology, Chinese Academy of Science, Beijing 100101, China.
FAU - Wang, Liu
AU  - Wang L
AD  - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing 100190, China.
AD  - Institute of Zoology, Chinese Academy of Science, Beijing 100101, China.
FAU - Liu, Zhong Hua
AU  - Liu ZH
AD  - College of Life Science, Northeast Agricultural University, Harbin, 150030, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Yi Chuan
JT  - Yi chuan = Hereditas
JID - 9436478
SB  - IM
MH  - *Cell Culture Techniques
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Umbilical Cord/*cytology
EDAT- 2018/12/19 06:00
MHDA- 2019/05/24 06:00
CRDT- 2018/12/19 06:00
PHST- 2018/12/19 06:00 [entrez]
PHST- 2018/12/19 06:00 [pubmed]
PHST- 2019/05/24 06:00 [medline]
AID - 10.16288/j.yczz.18-123 [doi]
PST - ppublish
SO  - Yi Chuan. 2018 Dec 20;40(12):1120-1128. doi: 10.16288/j.yczz.18-123.