PMID- 30563928
OWN - NLM
STAT- MEDLINE
DCOM- 20190823
LR  - 20230130
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 39
IP  - 1
DP  - 2019 Jan 31
TI  - MicroRNA-625 inhibits cell invasion and epithelial-mesenchymal transition by 
      targeting SOX4 in laryngeal squamous cell carcinoma.
LID - BSR20181882 [pii]
LID - 10.1042/BSR20181882 [doi]
AB  - INTRODUCTION: Laryngeal squamous cell carcinoma (LSCC) is a highly aggressive 
      malignant cancer, but the molecular mechanisms underlying its development and 
      progression remain largely elusive. The purpose of the present study is to 
      investigate the expression profile and functional role of microRNA-625 (miR-625) 
      in LSCC. MATERIALS AND METHODS: LSCC tissues and adjacent normal tissues were 
      collected from 86 LSCC patients. The expression levels of miR-625 and SOX4 mRNA 
      in tissues and cells were detected by RT-qPCR analysis. The expression levels of 
      SOX4 and EMT-related proteins were detected by western blot analysis. In vitro 
      cell proliferation, migration, and invasion were detected by MTT assay, colony 
      formation assay, wound healing assay, and transwell invasion assay, respectively. 
      Dual-luciferase reporter assay was performed to verify the binding relationship 
      between miR-625 and the 3'-UTR of SOX4. RESULTS: The results demonstrated that 
      miR-625 is significantly down-regulated in clinical LSCC tissues, and its low 
      expression may be closely associated with unfavorable clinicopathological 
      characteristics of LSCC patients. Overexpression of miR-625 significantly 
      suppressed the proliferation, migration, invasion, and EMT of LSCC cells. 
      Furthermore, SOX4 was validated as a direct target of miR-625 in LSCC cells, and 
      rescue experiments suggested that restoration of SOX4 blocked the tumor 
      suppressive role of miR-625 in LSCC cells. CONCLUSIONS: Taken together, these 
      findings highlighted a critical role of miR-625 in the pathogenesis of LSCC, and 
      restoration of miR-625 could be considered as a potential therapeutic strategy 
      against this fatal disease.
CI  - (c) 2019 The Author(s).
FAU - Li, Yuan
AU  - Li Y
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, Hangzhou 310015, China.
FAU - Tao, Chenjuan
AU  - Tao C
AD  - Department of Neurology, Affiliated Hospital of Hangzhou Normal University, 
      Hangzhou 310015, China.
FAU - Dai, Lili
AU  - Dai L
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, Hangzhou 310015, China.
FAU - Cui, Caixia
AU  - Cui C
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, Hangzhou 310015, China.
FAU - Chen, Chaohui
AU  - Chen C
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, Hangzhou 310015, China.
FAU - Wu, Honglin
AU  - Wu H
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, Hangzhou 310015, China.
FAU - Wei, Qingyu
AU  - Wei Q
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, Hangzhou 310015, China.
FAU - Zhou, Xuehua
AU  - Zhou X
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of 
      Hangzhou Normal University, Hangzhou 310015, China xuehuazhou22@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20190122
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (MIRN625 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (SOX4 protein, human)
RN  - 0 (SOXC Transcription Factors)
RIN - Biosci Rep. 2023 Jan 31;43(1):. PMID: 36714958
MH  - Aged
MH  - Carcinoma, Squamous Cell/genetics/*pathology
MH  - Cell Line, Tumor
MH  - Down-Regulation
MH  - Epithelial-Mesenchymal Transition/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - SOXC Transcription Factors/*genetics/metabolism
PMC - PMC6340973
OTO - NOTNLM
OT  - SOX4
OT  - epithelial-mesenchymal transition
OT  - laryngeal squamous cell carcinoma
OT  - microRNA-625
COIS- The author declares that there are no competing interests associated with the 
      manuscript.
EDAT- 2018/12/20 06:00
MHDA- 2019/08/24 06:00
PMCR- 2019/01/22
CRDT- 2018/12/20 06:00
PHST- 2018/10/17 00:00 [received]
PHST- 2018/11/26 00:00 [revised]
PHST- 2018/11/27 00:00 [accepted]
PHST- 2018/12/20 06:00 [pubmed]
PHST- 2019/08/24 06:00 [medline]
PHST- 2018/12/20 06:00 [entrez]
PHST- 2019/01/22 00:00 [pmc-release]
AID - BSR20181882 [pii]
AID - 10.1042/BSR20181882 [doi]
PST - epublish
SO  - Biosci Rep. 2019 Jan 22;39(1):BSR20181882. doi: 10.1042/BSR20181882. Print 2019 
      Jan 31.