PMID- 30564760
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2405-6383 (Electronic)
IS  - 2405-6545 (Print)
IS  - 2405-6383 (Linking)
VI  - 4
IP  - 4
DP  - 2018 Dec
TI  - Development of an in vitro protein digestibility assay mimicking the chicken 
      digestive tract.
PG  - 401-409
LID - 10.1016/j.aninu.2018.04.007 [doi]
AB  - It is difficult to obtain in vivo digestion kinetics data of high protein 
      ingredients using chickens. Collecting kinetics data requires repeated sampling 
      of digesta from the small intestine during the digestion process, which is not 
      easily accomplished due to the anatomical structure of chicken digestive tract. 
      An in vitro technique is proposed for measuring the digestion kinetics of protein 
      sources fed to chickens. The method has a 30 min gastric and 3 h intestinal 
      phase. Five hundred milligram crude protein (CP) equivalent of each meal sample 
      (CP = % N x 6.25) was digested with pepsin (28,260 units) in 50 mL polyethylene 
      centrifuge tubes for 30 min in a shaking water bath (150 strokes/min; 30 mm 
      stroke length) at 41  degrees C. The 6.5 mL pancreatin was selected as the enzyme 
      concentration for the intestinal phase, during which time 500 muL aliquots were 
      collected at 0, 15, 30, 45, 60, 90, 120, 150, 180 and 240 min. Samples were 
      diluted 1:820 with HCl and sodium acetate buffer, and then mixed with ninhydrin 
      reagent (2:1) at 100 +/- 2  degrees C for 15 min and spectrometric readings taken at 
      568 nm. To validate the assay, 5 replications of soybean meal (SBM), corn gluten 
      meal (CGM), corn distillers dried grains with solubles (CDDGS), porcine meal 
      (PCM), fish meal (FM) and casein (CA) were digested. The digestion data were 
      modeled with PROC NLIN procedure, and the intra coefficient of variation (CV) 
      assessed using PROC MEANS of SAS 9.4. The digestion values at 180 min were SBM 
      95 +/- 4, FM 93 +/- 3, PCM 68 +/- 4, CGM 82 +/- 3 and CDDGS 70 +/- 2. Intra CV for SBM, 
      CGM, CDDGS, PCM and FM were 5%, 5%, 12%, 10% and 2%, respectively. The estimated 
      fractional digestion rates for SBM, CGM, CDDGS, FM and PCM were 0.023, 0.013, 
      0.009, 0.024 and 0.013, respectively. In conclusion, the proposed in vitro 
      technique estimated the rate and extent of the digestion of CP for the meals with 
      low intra CV.
FAU - Bryan, Dervan D S L
AU  - Bryan DDSL
AD  - Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, 
      S7N 5A8, Saskatchewan, Canada.
FAU - Abbott, Dawn A
AU  - Abbott DA
AD  - Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, 
      S7N 5A8, Saskatchewan, Canada.
FAU - Classen, Henry L
AU  - Classen HL
AD  - Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, 
      S7N 5A8, Saskatchewan, Canada.
LA  - eng
PT  - Journal Article
DEP - 20180507
PL  - China
TA  - Anim Nutr
JT  - Animal nutrition (Zhongguo xu mu shou yi xue hui)
JID - 101717655
PMC - PMC6286628
OTO - NOTNLM
OT  - Corn distillers dried grains with solubles
OT  - Corn gluten meal
OT  - Fish meal
OT  - Porcine meal
OT  - Protein digestion rate
OT  - Soybean meal
EDAT- 2018/12/20 06:00
MHDA- 2018/12/20 06:01
PMCR- 2018/05/07
CRDT- 2018/12/20 06:00
PHST- 2018/03/16 00:00 [received]
PHST- 2018/04/17 00:00 [revised]
PHST- 2018/04/18 00:00 [accepted]
PHST- 2018/12/20 06:00 [entrez]
PHST- 2018/12/20 06:00 [pubmed]
PHST- 2018/12/20 06:01 [medline]
PHST- 2018/05/07 00:00 [pmc-release]
AID - S2405-6545(18)30073-8 [pii]
AID - 10.1016/j.aninu.2018.04.007 [doi]
PST - ppublish
SO  - Anim Nutr. 2018 Dec;4(4):401-409. doi: 10.1016/j.aninu.2018.04.007. Epub 2018 May 
      7.