PMID- 30564896 OWN - NLM STAT- MEDLINE DCOM- 20190305 LR - 20200225 IS - 1432-8798 (Electronic) IS - 0304-8608 (Print) IS - 0304-8608 (Linking) VI - 164 IP - 3 DP - 2019 Mar TI - Isolation and characterization of duck adenovirus 3 circulating in China. PG - 847-851 LID - 10.1007/s00705-018-4105-2 [doi] AB - Recently, infectious disease outbreaks characterized by swelling and hemorrhagic liver and kidneys occurred in Muscovy ducklings in China. Four viruses were isolated and identified as adenoviruses by transmission electron microscopy (TEM) and polymerase chain reaction (PCR). Sequence analysis identified the new isolates as duck adenovirus 3 (DAdV-3), species Duck aviadenovirus B. The pathogenicity of the new isolate DAdV-3 FJGT01 was investigated using challenge experiments. The gross lesions in the animal experiment were similar to the clinical lesions observed in the diseased ducks. TEM examination of liver sample showed that virions accumulated and arranged in crystal lattice formations in the nuclei of hepatocytes. The present study provides new information about the epidemiology and characteristics of duck adenovirus associated with Muscovy ducklings. FAU - Shi, Shaohua AU - Shi S AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. AD - Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, People's Republic of China. FAU - Liu, Rongchang AU - Liu R AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. AD - Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, People's Republic of China. FAU - Wan, Chunhe AU - Wan C AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. AD - Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, People's Republic of China. FAU - Cheng, Longfei AU - Cheng L AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. AD - Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, People's Republic of China. FAU - Chen, Zhen AU - Chen Z AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. AD - Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, People's Republic of China. FAU - Fu, Guanghua AU - Fu G AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. AD - Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, People's Republic of China. FAU - Chen, Hongmei AU - Chen H AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. AD - Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, People's Republic of China. FAU - Fu, Qiuling AU - Fu Q AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. AD - Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, People's Republic of China. FAU - Huang, Yu AU - Huang Y AD - Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, 247 Wusi Road, Fuzhou, Fujian, People's Republic of China. huangyu_815@163.com. AD - Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian, People's Republic of China. huangyu_815@163.com. LA - eng GR - 2017YFD0500802021/National Key Research and Development Program of China/ GR - 31472222/National Natural Science Foundation of China/ GR - CARS-42/Fund for Modern Agro-industry Technology Research System/ GR - 2017J01060/Natural Science Foundation of Fujian Province/ GR - 2016R1022-1/Basic Scientific Research Funds of Public Welfare Scientific Research Institutes of Fujian Province/ GR - 2016R1022-9/Basic Scientific Research Funds of Public Welfare Scientific Research Institutes of Fujian Province/ PT - Journal Article DEP - 20181218 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 SB - IM MH - Adenoviridae Infections/pathology/*veterinary/virology MH - Animals MH - Aviadenovirus/classification/genetics/*isolation & purification/pathogenicity MH - Ducks/*virology MH - Liver/pathology/virology MH - Phylogeny MH - Poultry Diseases/pathology/*virology MH - Virulence PMC - PMC6394704 COIS- CONFLICT OF INTEREST: All authors declare that they have no conflict of interest. ETHICAL APPROVAL: All animal experiments were approved by the animal welfare committee of Fujian Academy of Agricultural Sciences (no. 1306129FAAS). EDAT- 2018/12/20 06:00 MHDA- 2019/03/06 06:00 PMCR- 2018/12/18 CRDT- 2018/12/20 06:00 PHST- 2018/08/26 00:00 [received] PHST- 2018/11/05 00:00 [accepted] PHST- 2018/12/20 06:00 [pubmed] PHST- 2019/03/06 06:00 [medline] PHST- 2018/12/20 06:00 [entrez] PHST- 2018/12/18 00:00 [pmc-release] AID - 10.1007/s00705-018-4105-2 [pii] AID - 4105 [pii] AID - 10.1007/s00705-018-4105-2 [doi] PST - ppublish SO - Arch Virol. 2019 Mar;164(3):847-851. doi: 10.1007/s00705-018-4105-2. Epub 2018 Dec 18.