PMID- 30565961
OWN - NLM
STAT- MEDLINE
DCOM- 20200310
LR  - 20200310
IS  - 1559-7016 (Electronic)
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 39
IP  - 3
DP  - 2019 Mar
TI  - A silicon nanomembrane platform for the visualization of immune cell trafficking 
      across the human blood-brain barrier under flow.
PG  - 395-410
LID - 10.1177/0271678X18820584 [doi]
AB  - Here we report on the development of a breakthrough microfluidic human in vitro 
      cerebrovascular barrier (CVB) model featuring stem cell-derived brain-like 
      endothelial cells (BLECs) and nanoporous silicon nitride (NPN) membranes 
      (microSiM-CVB). The nanoscale thinness of NPN membranes combined with their high 
      permeability and optical transparency makes them an ideal scaffold for the 
      assembly of an in vitro microfluidic model of the blood-brain barrier (BBB) 
      featuring cellular elements of the neurovascular unit (NVU). Dual-chamber devices 
      divided by NPN membranes yield tight barrier properties in BLECs and allow an 
      abluminal pericyte-co-culture to be replaced with pericyte-conditioned media. 
      With the benefit of physiological flow and superior imaging quality, the microSiM-CVB 
      platform captures each phase of the multi-step T-cell migration across the BBB in 
      live cell imaging. The small volume of <100 microL of the microSiM-CVB will enable 
      in vitro investigations of rare patient-derived immune cells with the human BBB. 
      The microSiM-CVB is a breakthrough in vitro human BBB model to enable live and 
      high-quality imaging of human immune cell interactions with the BBB under 
      physiological flow. We expect it to become a valuable new tool for the study of 
      cerebrovascular pathologies ranging from neuroinflammation to metastatic cancer.
FAU - Mossu, Adrien
AU  - Mossu A
AUID- ORCID: 0000-0001-5986-2762
AD  - 1 Theodor Kocher Institute, University of Bern, Bern, Switzerland.
FAU - Rosito, Maria
AU  - Rosito M
AD  - 1 Theodor Kocher Institute, University of Bern, Bern, Switzerland.
FAU - Khire, Tejas
AU  - Khire T
AD  - 2 Department of Biomedical Engineering, University of Rochester, Rochester, NY, 
      USA.
FAU - Li Chung, Hung
AU  - Li Chung H
AD  - 2 Department of Biomedical Engineering, University of Rochester, Rochester, NY, 
      USA.
FAU - Nishihara, Hideaki
AU  - Nishihara H
AD  - 1 Theodor Kocher Institute, University of Bern, Bern, Switzerland.
FAU - Gruber, Isabelle
AU  - Gruber I
AD  - 1 Theodor Kocher Institute, University of Bern, Bern, Switzerland.
FAU - Luke, Emma
AU  - Luke E
AD  - 2 Department of Biomedical Engineering, University of Rochester, Rochester, NY, 
      USA.
FAU - Dehouck, Lucie
AU  - Dehouck L
AD  - 3 Blood Brain Barrier Laboratory, University of Artois, Lens, France.
FAU - Sallusto, Federica
AU  - Sallusto F
AD  - 4 Institute for Research in Biomedicine, Universita della Svizzera Italiana, 
      Bellinzona, Switzerland.
AD  - 5 Institute for Microbiology, ETH Zurich, Zurich, Switzerland.
FAU - Gosselet, Fabien
AU  - Gosselet F
AD  - 3 Blood Brain Barrier Laboratory, University of Artois, Lens, France.
FAU - McGrath, James L
AU  - McGrath JL
AD  - 2 Department of Biomedical Engineering, University of Rochester, Rochester, NY, 
      USA.
FAU - Engelhardt, Britta
AU  - Engelhardt B
AD  - 1 Theodor Kocher Institute, University of Bern, Bern, Switzerland.
LA  - eng
GR  - R01 HL125265/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20181219
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 0 (Membranes, Artificial)
RN  - 0 (Silicon Compounds)
RN  - QHB8T06IDK (silicon nitride)
SB  - IM
MH  - Blood-Brain Barrier/*metabolism
MH  - *Cell Movement
MH  - Coculture Techniques
MH  - Endothelial Cells/cytology
MH  - Humans
MH  - Immune System/*cytology
MH  - *Membranes, Artificial
MH  - Microfluidics/instrumentation/*methods
MH  - *Models, Biological
MH  - Nanotechnology/methods
MH  - Pericytes/cytology
MH  - Permeability
MH  - Silicon Compounds
PMC - PMC6421249
OTO - NOTNLM
OT  - Blood-brain barrier
OT  - T-cell migration
OT  - microfluidics
OT  - nanoporous silicon nitride membrane
OT  - two-compartmental flow chamber
EDAT- 2018/12/20 06:00
MHDA- 2020/03/11 06:00
PMCR- 2020/03/01
CRDT- 2018/12/20 06:00
PHST- 2018/12/20 06:00 [pubmed]
PHST- 2020/03/11 06:00 [medline]
PHST- 2018/12/20 06:00 [entrez]
PHST- 2020/03/01 00:00 [pmc-release]
AID - 10.1177_0271678X18820584 [pii]
AID - 10.1177/0271678X18820584 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2019 Mar;39(3):395-410. doi: 10.1177/0271678X18820584. 
      Epub 2018 Dec 19.