PMID- 30566388
OWN - NLM
STAT- MEDLINE
DCOM- 20190405
LR  - 20190405
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Linking)
VI  - 191
IP  - 2
DP  - 2019 Feb
TI  - Dose-Dependent Increase of Nrf2 Target Gene Expression in Mice Exposed to 
      Ionizing Radiation.
PG  - 176-188
LID - 10.1667/RR15203.1 [doi]
AB  - Nuclear factor-erythroid-2-related factor 2 transcription factor (Nrf2) is 
      activated by reactive oxygen species (ROS) and binds to antioxidant response 
      elements in the promoter regions of its target genes involved in redox regulation 
      and antioxidative functions. In this study, we elucidated the relationship 
      between radiation dose and the expression response of Nrf2 target genes involved 
      in oxidative stress, such as heme oxygenase 1, ferritin heavy polypeptide 1 ( 
      Fth1), NADPH dehydrogenase quinone 1, glutamate-cysteine ligase catalytic 
      subunit, glutamate-cysteine ligase modifier subunit, glutathione reductase ( Gsr) 
      and thioredoxin reductase 1 genes, in peripheral blood from X-ray irradiated 
      mice. Whole-body radiation doses ranged from 0.5 to 3 Gy, and gene expressions 
      were analyzed using reverse transcription quantitative polymerase chain reaction. 
      A significant relationship was observed only for one gene: a statistically 
      significant positive correlation between radiation dose and Fth1 mRNA expression 
      was detected. However, Fth1 did not show any correlations with the biological 
      damages induced by radiation tested in this study. Furthermore, while Gsr 
      expression was significantly associated with spleen weight loss, splenic cell 
      number reduction and bone marrow cell death apoptosis, no significant correlation 
      was observed between Gsr expression and radiation dose. Together these results 
      indicate that Nrf2 target gene expression is closely related to radiation dose 
      and its level may reflect biological damages induced by ionizing radiation. These 
      findings suggest the possibility for application of these target genes as a 
      bio-dosimeter and/or damage marker in individuals exposed to ionizing radiation.
FAU - Miura, Shuta
AU  - Miura S
AD  - a Department of Radiology, Akita Kousei Medical Center, Akita 011-0948, Japan.
FAU - Yamaguchi, Masaru
AU  - Yamaguchi M
AD  - b Department of Radiation Science, Hirosaki University Graduate School of Health 
      Sciences, Aomori 036-8564, Japan.
FAU - Yoshino, Hironori
AU  - Yoshino H
AD  - b Department of Radiation Science, Hirosaki University Graduate School of Health 
      Sciences, Aomori 036-8564, Japan.
FAU - Nakai, Yuji
AU  - Nakai Y
AD  - c Institute for Food Sciences, Hirosaki University, Aomori 038-0012, Japan.
FAU - Kashiwakura, Ikuo
AU  - Kashiwakura I
AD  - b Department of Radiation Science, Hirosaki University Graduate School of Health 
      Sciences, Aomori 036-8564, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181219
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/radiation effects
MH  - Cell Death/radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Gene Expression Regulation/*radiation effects
MH  - Mice
MH  - NF-E2-Related Factor 2/*genetics
MH  - Organ Size/radiation effects
MH  - Oxidative Stress/genetics
MH  - *Radiation, Ionizing
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spleen/radiation effects
MH  - Whole-Body Irradiation
EDAT- 2018/12/20 06:00
MHDA- 2019/04/06 06:00
CRDT- 2018/12/20 06:00
PHST- 2018/12/20 06:00 [pubmed]
PHST- 2019/04/06 06:00 [medline]
PHST- 2018/12/20 06:00 [entrez]
AID - 10.1667/RR15203.1 [pii]
AID - 10.1667/RR15203.1 [doi]
PST - ppublish
SO  - Radiat Res. 2019 Feb;191(2):176-188. doi: 10.1667/RR15203.1. Epub 2018 Dec 19.