PMID- 30568257
OWN - NLM
STAT- MEDLINE
DCOM- 20191023
LR  - 20200309
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Dec 19
TI  - Physiological and proteomic analysis on long-term drought resistance of cassava 
      (Manihot esculenta Crantz).
PG  - 17982
LID - 10.1038/s41598-018-35711-x [doi]
LID - 17982
AB  - Drought stress is one of the potent abiotic stress limiting cassava (Manihot 
      esculenta) yield globally, but studies addressing both physiological and 
      proteomic responses that how cassava crops can adjust their growth and metabolism 
      under drought conditions are lacking. Combining leaf physiological and proteomic 
      characteristics strongly allied with drought tolerance should results in enhanced 
      drought tolerance in cassava crop. Therefore, the aims of this study were to 
      explore the plant physiological and proteomic mechanisms involved in drought 
      adaptation in cassava. Xinxuan 048 (XX048) was exposed to well-watered control 
      (CK, relative soil water content (RSWC) as 80 +/- 5%), mild drought stress (LD, 
      RSWC as 65 +/- 5%), moderate drought stress (MD, RSWC as 50 +/- 5%) and severe 
      drought stress (SD, RSWC as 35 +/- 5%) from 30 days after planting. Under drought 
      stress conditions, cassava plant showed a substantial decline in plant height, 
      stem diameter, leaf number, leaf water content, the ratio of free water content 
      to bound water content of leaf (FW/BW), net photosynthetic rate (Pn), 
      intercellular CO(2) concentration (Ci), stomatal conductance (Gs) and 
      transpiration rate (Tr) compared with well watered plants. However, compared with 
      control, leaf water content, SPAD value, cell membrane permeability, 
      malondialdehyde (MDA), soluble sugar, protein proline content SOD and CAT 
      activity were at peak under drought stress. The proteomic analysis revealed that 
      among 3 339 identified proteins, drought stress increased and decreased abundance 
      of 262 and 296 proteins, respectively, compared with control condition. These 
      proteins were involved in carbohydrate energy metabolism, protein homeostasis, 
      transcription, cell structure, cell membrane transport, signal transduction, 
      stress and defense responses. These data not only provides a comprehensive 
      dataset on overall proteomic changes in cassava leaves under drought stress, but 
      also highlights the mechanisms by which euphorbiaceae plants can adapt to drought 
      conditions.
FAU - Shan, Zhongying
AU  - Shan Z
AUID- ORCID: 0000-0003-3775-2356
AD  - College of Agronomy, Guangxi University, Nanning, 530004, China.
FAU - Luo, Xinglu
AU  - Luo X
AD  - College of Agronomy, Guangxi University, Nanning, 530004, China. 
      luoxinglu@sina.com.
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-bioresources, Nanning, 530004, China. luoxinglu@sina.com.
FAU - Wei, Maogui
AU  - Wei M
AD  - College of Agronomy, Guangxi University, Nanning, 530004, China.
FAU - Huang, Tangwei
AU  - Huang T
AD  - College of Agronomy, Guangxi University, Nanning, 530004, China.
FAU - Khan, Aziz
AU  - Khan A
AD  - College of Agronomy, Guangxi University, Nanning, 530004, China.
FAU - Zhu, Yanmei
AU  - Zhu Y
AD  - College of Agronomy, Guangxi University, Nanning, 530004, China.
LA  - eng
GR  - 2010GXNSFD013025/Natural Science Foundation of Guangxi Province (Guangxi Natural 
      Science Foundation)/International
GR  - 2010GXNSFD013025/Natural Science Foundation of Guangxi Province (Guangxi Natural 
      Science Foundation)/International
GR  - 2010GXNSFD013025/Natural Science Foundation of Guangxi Province (Guangxi Natural 
      Science Foundation)/International
GR  - 2010GXNSFD013025/Natural Science Foundation of Guangxi Province (Guangxi Natural 
      Science Foundation)/International
GR  - U1033004-09/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
GR  - GKG1222014/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
GR  - U1033004-09/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
GR  - U1033004-09/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181219
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Plant Proteins)
RN  - 0 (Proteome)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - *Adaptation, Physiological
MH  - Cell Membrane Permeability
MH  - Computational Biology/methods
MH  - *Droughts
MH  - Gene Expression Regulation, Plant
MH  - Gene Ontology
MH  - Manihot/*physiology
MH  - Plant Proteins/genetics/metabolism
MH  - Protein Interaction Mapping
MH  - Protein Interaction Maps
MH  - *Proteome
MH  - *Proteomics/methods
MH  - Stress, Physiological
MH  - Water/metabolism
PMC - PMC6299285
COIS- The authors declare no competing interests.
EDAT- 2018/12/21 06:00
MHDA- 2019/10/24 06:00
PMCR- 2018/12/19
CRDT- 2018/12/21 06:00
PHST- 2018/04/24 00:00 [received]
PHST- 2018/11/09 00:00 [accepted]
PHST- 2018/12/21 06:00 [entrez]
PHST- 2018/12/21 06:00 [pubmed]
PHST- 2019/10/24 06:00 [medline]
PHST- 2018/12/19 00:00 [pmc-release]
AID - 10.1038/s41598-018-35711-x [pii]
AID - 35711 [pii]
AID - 10.1038/s41598-018-35711-x [doi]
PST - epublish
SO  - Sci Rep. 2018 Dec 19;8(1):17982. doi: 10.1038/s41598-018-35711-x.